Identification of the powdery mildew caused by Erysiphe cruciferarum on Brassica napus in Russia

2021 ◽  
Author(s):  
Elena Mikhaylova ◽  
Michael Shein ◽  
Valentin Alexeev ◽  
Anna Sukhareva ◽  
Maria Panfilova
Keyword(s):  
Brassica Napus ◽  
Powdery Mildew ◽  
Erysiphe Cruciferarum

scholarly journals First Report of Powdery Mildew Caused by Erysiphe cruciferarum on Brassica napus in China

Plant Disease ◽  
2015 ◽  
Vol 99 (11) ◽  
pp. 1651-1651 ◽  
Author(s):  
J. T. Alkooranee ◽  
S. Liu ◽  
T. R. Aledan ◽  
Y. Yin ◽  
M. Li
Keyword(s):  
Brassica Napus ◽  
Powdery Mildew ◽  
First Report ◽  
Erysiphe Cruciferarum

Cultivar resistance offers the first opportunity for effective management of the emerging powdery mildew (Erysiphe cruciferarum) threat to oilseed brassicas in Australia

2016 ◽  
Vol 67 (11) ◽  
pp. 1179 ◽  
Author(s):  
M. B. Uloth ◽  
M. P. You ◽  
M. J. Barbetti
Keyword(s):  
Brassica Napus ◽  
Powdery Mildew ◽  
Effective Management ◽  
Cultivar Resistance ◽  
Resistant Cultivars ◽  
Progress Curve ◽  
Erysiphe Cruciferarum ◽  
Effective Option ◽  
Oilseed Brassicas ◽  
The Impact

Powdery mildew of brassicas, caused by Erysiphe cruciferarum, is an emerging threat to oilseed Brassica production in Australia. Resistance to powdery mildew was assessed in 112 current and historic Australian Brassica napus canola cultivars and five cultivars of B. juncea mustard cultivars under controlled environmental conditions. Only 18% of leaf area was infested by the end of the test on the most resistant cultivars, compared with means of up to 70% for the most susceptible cultivars as well as severe stem and pod infection. For B. napus, cultivars with the greatest potential for reducing the impact of powdery mildew in the field were Trooper, Bravo TT, Summit, Tumby, Narendra and Hyola 650TT, all ranked in the 10% of cultivars with the lowest leaf infestation (Area Under The Disease Progress Curve (AUDPC) <537) and with <10% of stem area infested. For B. juncea, the level of leaf infestation was lowest for Sahara CL and Xceed X121 CL (AUDPC 303 and 380 respectively), but the high levels of stem infestation (42% and 28% respectively) in these cultivars may reduce their usefulness in the field. The most resistant cultivars identified can be immediately deployed into regions where powdery mildew is prevalent, providing the canola industry with an immediate and effective option for management of this increasingly troublesome disease.

scholarly journals Multiple Evolutionary Events Involved in Maintaining Homologs of Resistance to Powdery Mildew 8 in Brassica napus

2016 ◽  
Vol 7 ◽  
Author(s):  
Qin Li ◽  
Jing Li ◽  
Jin-Long Sun ◽  
Xian-Feng Ma ◽  
Ting-Ting Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Powdery Mildew

First Report of Powdery Mildew of Eschscholzia californica (California Poppy) caused by Erysiphe cruciferarum in North America

2006 ◽  
Vol 7 (1) ◽  
pp. 45 ◽  
Author(s):  
Dean A. Glawe
Keyword(s):  
North America ◽  
Powdery Mildew ◽  
Pacific Northwest ◽  
Ongoing Study ◽  
Annual Species ◽  
Powdery Mildew Fungus ◽  
First Report ◽  
California Poppy ◽  
The Pacific ◽  
Erysiphe Cruciferarum

California poppy is an annual species grown widely in the Pacific Northwest. Once established, populations are self-seeding and require little care. During an ongoing study of Erysiphales, a powdery mildew fungus was collected repeatedly on this species in Seattle, WA. The fungus was determined to be Erysiphe cruciferarum Opiz ex Junell, a species not reported previously on this host in North America. This report documents the occurrence of the disease and provides information on the morphology and identification of the causal agent. Accepted for publication 8 November 2006. Published 13 December 2006.

scholarly journals First report of Erysiphe cruciferarum causing powdery mildew of Alliaria petiolata in Maryland

Plant Disease ◽  
2021 ◽  
Author(s):  
Michael Robert Fulcher ◽  
Paul C Owen-Smith
Keyword(s):  
Powdery Mildew ◽  
Invasive Plant ◽  
Local Population ◽  
Alliaria Petiolata ◽  
Specific Information ◽  
Garlic Mustard ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
Erysiphe Cruciferarum ◽  
Disease Free

Alliaria petiolata (Bieb.) Cavara & Grande (garlic mustard) is a biennial crucifer native to Europe and invasive in North America, where it outcompetes native plants in deciduous forests. In July 2021, powdery mildew was observed on A. petiolata in Frederick County, Maryland. Signs of the disease included white, tomentose mycelium producing abundant conidia (Fig S1). A majority of plants were affected, and severity ranged from the presence of small, discrete infections to complete colonization of leaves, stems, and ripening seed pods. Conidia from field collected leaves were transferred to disease-free A. petiolata for maintenance in a growth chamber at 20°C and 80% RH with a 12 hr photoperiod. Fungal morphology was recorded 30 days after this transfer. Appressoria were irregularly lobed, and conidiophores were straight and composed of 2-3 cells. Cylindrical to oblong conidia were produced singly in pseudochains of 2-6 (x̄ = 3), measured 39-64 by 18-29 (x̄ = 52 by 24) μm, had a length to width ratio greater than two, and germinated at the ends. Fibrosin bodies were absent from conidia, and chasmothecia were not observed in the field or on inoculated plant material. Based on anamorphic characteristics, the pathogen was placed in the genus Erysiphe (Boesewinkel 1980). Species level identity was determined using DNA sequences. Conidia and mycelia were scraped from leaves and used for genomic DNA extraction with the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA). A portion of the internal transcribed spacer region of rDNA was amplified using the primers ITS5/ITS4 (White et al. 1990). Purified amplicons (PCR & DNA Cleanup Kit, New England BioLabs Inc., Ipswich, MA) were sequenced at Eurofins Genomics (Louisville, KY). The resulting sequence was compared to those in NCBI GenBank using the blastn algorithm (Altschul et al. 1990). The newly generated sequence (GenBank: OK157430) was identical (599/599 bp) to samples of E. cruciferarum from the United Kingdom (GenBank: KY660931.1, KY660879.1, KY660752.1). Because E. cruciferarum sensu lato is heterogeneous (Pastirčáková et al. 2016), additional sequence comparisons were made to the E. cruciferarum sensu stricto holotype (589/599 bp, GenBank: KU672364) and a vouchered E. cruciferarum s. lat. (596/599 bp, GenBank: LC009943). This supports identification of the pathogen as E. cruciferarum s. lat. and suggests the taxonomy of isolates from A. petiolata should be reassessed following any revision to E. cruciferarum. A modified Koch’s postulates procedure was followed to confirm pathogenicity. Leaves colonized by E. cruciferarum were briefly pressed against the leaves of three disease-free plants grown from seed in a greenhouse. After 14 days, inoculated plants showed signs of powdery mildew similar to those observed in the field, and a control treatment using pathogen-free leaves resulted in no disease. This inoculation experiment was performed twice, and the identity of the pathogen was reconfirmed based on morphology. This is the first report of powdery mildew on A. petiolata in Maryland. Erysiphe cruciferarum s. lat. is widely distributed on other hosts and has been found on A. petiolata throughout Europe and in Ohio and Indiana (Farr and Rossman 2021; Blossey et al. 2001; Enright and Cipollini 2007; Ciola and Cipollini 2011). This pathogen has been proposed as a biological control agent (Cipollini and Enright 2009; Cipollini et al. 2020), and the presence of disease in Maryland suggests the local population of A. petiolata is susceptible to E. cruciferarum and the environment there is favorable to disease development. References: Altschul, S. F., et al. 1990. J. Mol. Biol. 215:403. Blossey, B., et al. 2001. Nat. Areas J. 21:357. Boesewinkel, H. J. 1980. Bot. Rev. 46:167. Ciola, V., and Cipollini, D. 2011. Am. Midl. Nat. 166:40-52. Cipollini, D., and Enright, S. M. 2009. Invasive Plant Sci. Manag. 2:253. Cipollini, D., et al. 2020. Biol. Invasions. 22:1657-1668. Enright, S. M., and Cipollini, D. 2007. Am. J. Bot. 94:1813. Farr, D. F., and Rossman, A. Y. 2021. Fungal Databases, Syst. Mycol. Microbial. Lab., ARS, USDA. https://nt.ars-grin.gov/fungaldatabases/ Pastirčáková, K., et al. 2016. Mycol. Prog. 15:36 White, T. J., et al. 1990. Page 315 in: PCR Protocols. A Guide to Methods and Applications, Academic Press, San Diego. Keywords: Alliaria petiolata, Erysiphe cruciferarum, garlic mustard, powdery mildew, invasive plant, biocontrol Funding and Disclaimer: The author(s) declare no conflict of interest. This work was supported by USDA-ARS Appropriated Project Number 8044-22000-047-000-D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity lender, provider, and employer.

scholarly journals First Report of Powdery Mildew Caused by Erysiphe cruciferarum on Camelina sativa in Montana

Plant Disease ◽  
2021 ◽  
Author(s):  
Benzhong Fu ◽  
Qing Yan
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
The United States ◽  
Camelina Sativa ◽  
Its Sequences ◽  
Flowering Plant ◽  
Biofuel Crop ◽  
First Report ◽  
Erysiphe Cruciferarum ◽  
Query Cover

Camelina sativa (L.) Crantz, also known as false flax, is an annual flowering plant in the family Brassicaceae and originated in Europe and Asia. In recent years, it is cultivated as an important biofuel crop in Europe, Canada, and the northwest of the United States. In June of 2021, severe powdery mildew was observed on C. sativa ‘Suneson’ plants under greenhouse conditions (temperature 18.3°C/22.2°C, night/day) in Bozeman, Montana (45°40'N, 111°2'W). The disease incidence was 80.67% (150 pots, one plant per pot). White ectophytic powdery mildew including mycelia and conidia were observed on the upper leaves, usually developed from bottom tissues to top parts, also present on stems and siliques. Mycelia on leaves were amphigenous and in patches, often spreading to become effused. These typical symptoms were similar to a previous report of powdery mildew on Broccoli raab (Koike and Saenz 1997). Appressoria are lobed, and foot cells are cylindrical with size 18 to 26 × 7 to 10 μm. Conidia are cylindrical and produced singly, with a size of 35 to 50 × 12 to 21 μm and a length : width ratio greater than two (Koike and Saenz 1997). No chasmothecia were observed under the greenhouse conditions. The symptoms and fungal microscopic characters are typical of Pseudoidium anamorph of Erysiphe (Braun 1995). The specific measurements and characteristics are consistent with previous records of Erysiphe cruciferarum Opiz ex L. Junell (Braun and Cook 2012; Vellios et al. 2017). To identify the pathogen, the partial internal transcribed spacer (ITS) region of rDNA of sample CPD-1 was amplified using primers ITS1 and ITS4 (White et al. 1990). The amplicons were sequenced, and the resulting 559-bp sequence was deposited in GenBank (CPD-1, Accession number: OK160719). A GenBank BLAST search of the ITS sequences showed an exact match (100% query cover, E-value 0, and 100% identity 559/559 bp) with those of E. cruciferarum on hosts Brassica sp. (KY660929.1), B. juncea from Vietnam (KM260718.1) and China (KT957424.1). A phylogenetic tree was generated with the CPD-1 ITS sequence with several of ITS sequences of close species with different hosts obtained from the GenBank. Isolate CPD-1 was grouped with pathogens from Brassica hosts rather than the holotype strain (KU672364.1) from papaveraceous hosts. To fulfill Koch's postulates, pathogenicity was confirmed through inoculation by dusting conidia onto leaves of seven healthy, potted, 14-day-old C. sativa seedlings (cv. Suneson). Seven non-inoculated plants served as a control treatment. The plants were incubated in a greenhouse with a temperature of 18°C (night) to 22°C (day). The inoculated plants developed similar symptoms after 7 days, whereas the control plants remained symptomless. The fungus on the inoculated plants was morphologically identical to that was originally observed on the diseased plants. Though many Brassica spp. have been known to be infected by E. cruciferarum throughout the world, powdery mildew of C. sativa cultivar Crantz in natural conditions by E. cruciferarum has been reported only in the province of Domokos in Central Greece (Vellios et al. 2017). To our knowledge, this is the first report of powdery mildew caused by E. cruciferarum on C. sativa in Montana. Though the powdery mildew on C. sativa was observed in the greenhouse conditions in this work, it poses a potential threat to the production of this biofuel crop in the northwest of the United States.

Sign in / Sign up

Export Citation Format

Share Document