Diagnostics of the bacterial blight pathogen of bean Xanthomonas axonopodis pv. Phaseoli in plant and seed material of grain legumes using molecular genetics methods

2021 ◽  
Author(s):  
I. M. Ignatyeva ◽  
E. V. Karimova ◽  
S. I. Prikhodko
Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 448-455 ◽  
Author(s):  
Defu Wang ◽  
Yajun Wang ◽  
Maoqiang Fu ◽  
Shuyuan Mu ◽  
Bing Han ◽  
...  

Powdery mildew, one of devastating diseases of wheat worldwide, is caused by Erysiphe graminis f. sp. tritici, a fungal species with constant population changes, which often poses challenges in disease management with host resistance. Transgenic approaches that utilize broad-spectrum resistance may limit changes of pathogen populations and contribute to effective control of the disease. The harpin protein Hpa1, produced by the rice bacterial blight pathogen, can induce resistance to bacterial blight and blast in rice. The fragment comprising residues 10 through 42 of Hpa1, Hpa110-42, is reportedly three- to eightfold more effective than the full-length protein. This study evaluated the transgenic expression of the Hpa110-42 gene for resistance to powdery mildew in wheat caused by E. graminis f. sp. tritici. Nine Hpa110-42 transgenic wheat lines were generated. The genomic integration of Hpa110-42 was confirmed, and expression of the transgene was detected at different levels in the individual transgenic lines. Following inoculation with the E. graminis f. sp. tritici isolate Egt15 in the greenhouse, five transgenic lines had significantly higher levels of resistance to powdery mildew compared with nontransformed plants. Thus, transgenic expression of Hpa110-42 conferred resistance to one isolate of E. graminis f. sp. tritici in wheat in the greenhouse.


2010 ◽  
Vol 158 (4) ◽  
pp. 288-295 ◽  
Author(s):  
Sascha D. Braun ◽  
Janine Hofmann ◽  
Annette Wensing ◽  
Helge Weingart ◽  
Matthias S. Ullrich ◽  
...  

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.


1990 ◽  
Vol 40 (3) ◽  
pp. 329-338
Author(s):  
Tsugufumi OGAWA ◽  
Tsuyoshi YAMAMOTO ◽  
Gurdev S. KHUSH ◽  
Twng-Wah MEW

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