The phenotypic and quantitative detection of the pyocyanin stain in multiple antibiotic resistance Pseudomonas aeruginosa isolated from different clinical infections

2022 ◽  
Author(s):  
Ahmed Yas Saeed ◽  
Sabah Ahmed Naif Aljubori ◽  
Mahmood Khlaf Saleh ◽  
Mostafa Qahtan Mostafa ◽  
Nedaa Wasmi Shehab
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Quantitative Detection ◽  
Multiple Antibiotic Resistance

scholarly journals Negative Regulation of the Pseudomonas aeruginosa Outer Membrane Porin OprD Selective for Imipenem and Basic Amino Acids

1999 ◽  
Vol 43 (5) ◽  
pp. 1085-1090 ◽  
Author(s):  
Martina M. Ochs ◽  
Matthew P. McCusker ◽  
Manjeet Bains ◽  
Robert E. W. Hancock
Keyword(s):  
Amino Acids ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Clinical Effectiveness ◽  
Multiple Antibiotic Resistance ◽  
Outer Membranes ◽  
Basic Amino Acids ◽  
Outer Membrane Porin ◽  
Strong Homology ◽  
Imipenem Resistance

ABSTRACT Pseudomonas aeruginosa OprD is a specific porin which facilitates the uptake of basic amino acids and imipenem, a carbapenem antibiotic. Resistance to imipenem due to the loss of OprD is an important mechanism for the loss of clinical effectiveness. To investigate the negative regulatory mechanisms influencingoprD expression, a gene upstream of the coregulatedmexEF-oprN efflux operon, designated mexT, was cloned. The predicted 304-amino-acid mature MexT protein showed strong homology to LysR-type regulators. When overexpressed it induced the expression of the mexEF-oprN efflux operon while decreasing the level of expression of OprD. The use of anoprD::xylE transcriptional fusion indicated that it acted by repressing the transcription ofoprD. Salicylate, a weak aromatic acid known to reduce porin expression and induce low levels of multiple antibiotic resistance in Escherichia coli, was able to induce imipenem resistance and reduce the expression of OprD but not multiple antibiotic resistance or OprN expression in P. aeruginosa. This was also demonstrated to occur at the level of transcription. Acetyl salicylate and benzoate, but not catechol, were also able to reduce the levels of OprD in the P. aeruginosa outer membranes. These OprD-suppressing compounds increased imipenem resistance even in a mexT-overexpressing andnfxC mutant backgrounds, suggesting that such resistance is independent of the MexT repressor and that oprD is influenced by more than a single mechanism of repression.

scholarly journals Immunization and Immunotherapy Approaches against Pseudomonas aeruginosa and Burkholderia cepacia Complex Infections

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 670
Author(s):  
Sílvia A. Sousa ◽  
António M. M. Seixas ◽  
Joana M. M. Marques ◽  
Jorge H. Leitão
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Trials ◽  
Antibiotic Resistance ◽  
Burkholderia Cepacia ◽  
Passive Immunization ◽  
Burkholderia Cepacia Complex ◽  
Rational Approach ◽  
Multiple Antibiotic Resistance ◽  
Vaccination Strategies ◽  
Human Infections

Human infections caused by the opportunist pathogens Burkholderia cepacia complex and Pseudomonas aeruginosa are of particular concern due to their severity, their multiple antibiotic resistance, and the limited eradication efficiency of the current available treatments. New therapeutic options have been pursued, being vaccination strategies to prevent or limit these infections as a rational approach to tackle these infections. In this review, immunization and immunotherapy approaches currently available and under study against these bacterial pathogens is reviewed. Ongoing active and passive immunization clinical trials against P. aeruginosa infections is also reviewed. Novel identified bacterial targets and their possible exploitation for the development of immunization and immunotherapy strategies against P. aeruginosa and B. cepacia complex and infections are also presented and discussed.

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