Isolation and identification of some bacterial species from dialysis unit in Basrah

2022 ◽  
Author(s):  
Hanadi Abdulqader Jasim ◽  
Raghad Shreif Ibrahim ◽  
Basil Abdulzahra Abbas ◽  
Bashir Ali ◽  
Qasim Najim ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Isolation And Identification ◽  
Dialysis Unit

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

Isolation and Identification of Multidrug-Resistant Isolates of Uropathogenic Klebsiella spp in Dogs

2021 ◽  
Vol 3 (1) ◽  
pp. 6-12
Author(s):  
M Mustapha ◽  
P Goel
Keyword(s):  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Penicillin G ◽  
Isolation And Identification ◽  
Sensitivity Testing ◽  
Bacterial Organism ◽  
Tract Infections ◽  
Klebsiella Spp

The most widespread ailments in dogs are urinary tract infections (UTIs) caused by bacterial species. It is necessary to recognize the prevailing bacterial pathogens and their susceptibility to antimicrobial agents to effectively treat UTIs. The present study aimed to classify the bacterial organism that causes UTIs in dogs and their patterns of antimicrobial resistance. A total of 141 urine samples were collected from diseased dogs in Veterinary Clinical Complex LUVAS in Hisar, India. Culture, biochemical and sensitivity testing were performed for each of the urine samples based on standard method. Of the total 141 urine samples from dogs, 21 (14.9%) isolates were identified as Klebsiella spp. The isolates were found to be highly resistant to ampicillin (100%), penicillin G (100%), oxytetracycline (100%), enrofloxacin (85.7%), chloramphenicol (80.6%), ceftriaxone (76.2%) and cloxacillin (71.4%), while susceptibility was observed against gentamicin (100%), amikacin (100%) and neomycin (90.5%). In the current study, 19 out of 21 identified isolates were found to be multidrug-resistant. This study indicates that dogs in the study area are found to harbor highly resistant Klebsiella spp. Therefore, when deciding on the antibiotic regimen for UTIs cases, Vets should consider resistance profile of chosen antibacterial agents before usage in order to discourage dissemination of resistant organisms in the study area.

scholarly journals Isolation and identification of bacteria and parasites in glass eel (Anguilla spp.)

2021 ◽  
Vol 322 ◽  
pp. 02012
Author(s):  
Septyan Andriyanto ◽  
Hessy Novita ◽  
Tuti Sumiati ◽  
Taukhid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prevalence Rate ◽  
Bacterial Species ◽  
Bacterial Identification ◽  
Biochemical Method ◽  
Isolation And Identification ◽  
Glass Eel ◽  
Pathogenic Agent ◽  
Identification Of Bacteria ◽  
Glass Eels

The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogyrus spp. (2,70%) and Gyrodactylus spp. (2,70%). Bacterial and parasites identified in glass eels need further verification on the epizootiology characteristic of each pathogenic agent.

scholarly journals Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods

2017 ◽  
Vol 11 (1) ◽  
pp. 384-386 ◽  
Author(s):  
Mubarak Alfaresi ◽  
Bassam Mahboub
Keyword(s):  
Cystic Fibrosis ◽  
Bacterial Species ◽  
Transmembrane Conductance Regulator ◽  
Bacterial Identification ◽  
Molecular Method ◽  
Isolation And Identification ◽  
Pcr Product ◽  
Clinical Culture ◽  
Identification Of Bacteria ◽  
Cf Transmembrane Conductance Regulator

Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing. Results: All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning. Conclusion: This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.

Development of Isothermal LAMP Assay for Detection of Intimin Gene (eae) in E. coli Associated with Piglet Diarrhea

2020 ◽  
Author(s):  
Sanjeev Kumar ◽  
Jagan Mohanarao Gali ◽  
T.K. Dutta ◽  
P. Roychoudhury ◽  
P.K. Subudhi
Keyword(s):  
Bacterial Species ◽  
False Negative ◽  
Acute Diarrhoea ◽  
Lamp Assay ◽  
Dna Amplification ◽  
Analytical Sensitivity ◽  
Conventional Pcr ◽  
Isolation And Identification ◽  
Reliable Technique ◽  
E Coli

Background: Diarroeagenic Escherichia coli (DEC) including enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) is associated with acute diarrhoea in children and young animals. The virulence is associated with attaching and effacing lesions encoded by eaeA gene is considered as marker for EPEC and EHEC. Laboratory diagnosis of such infections is carried out by traditional bacteriological techniques and by conventional PCR assays. Those techniques often provide false negative result and at the same time are costly as well as difficult to perform in the field level. The loop-mediated isothermal amplification (LAMP) is a new generation DNA amplification assay is developed for detection of eaeA gene in E. coli isolated from diarrhoeic piglets.Methods: Samples were collected from diarrhoeic piglets for isolation and identification of E. coli. eaeA gene was detected by conventional PCR using specific primers in all the isolates. LAMP assay was standardized for detection of eaeA gene. Analytical sensitivity of LAMP was evaluated using 10 fold serially diluted E. coli genomic DNA. The specificity of the LAMP assay was determined by evaluating the cross reactivity with 19 other enteric and non-enteric bacterial species. Standardized LAMP was applied for detection of eaeA gene in the field isolates.Result: A total of 37 (24.67%) isolates were recorded as positive for eaeA gene by conventional PCR, while 49 (32.67%) isolates were recorded as positive for eaeA gene by LAMP assay. The LAMP assay was 10 times more sensitive than conventional PCR. LAMP assay was found to be more sensitive, specific, cost effective, user friendly and reliable technique over conventional PCR, which can be applied for screening of the clinical isolates for confirmation of EPEC and/or EHEC.

scholarly journals Isolation and Identification of the Causal Agent of Brown Stalk Rot, A New Disease of Maize in South Africa

Plant Disease ◽  
2007 ◽  
Vol 91 (6) ◽  
pp. 711-718 ◽  
Author(s):  
T. Goszczynska ◽  
W. J. Botha ◽  
S. N. Venter ◽  
T. A. Coutinho
Keyword(s):  
South Africa ◽  
Biochemical Characterization ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Rrna Gene ◽  
New Disease ◽  
Isolation And Identification ◽  
Stalk Rot ◽  
Pathogenicity Tests ◽  
Internal Browning

During 2004 to 2005, an unreported disease of maize (Zea mays) was observed on commercial fields in the Northwest and Mpumalanga Provinces of South Africa. Infected plants were stunted, with a vertical crack at the first internode. Inside the stem, a dark-brown, narrow lesion was present along the crack. Internal browning inside the stem extended upward, reaching the top internode in some plants. Seed cobs were underdeveloped. Diseased plants were scattered in the fields and 10 to 70% of the crop was affected. Gram-negative, facultatively anaerobic bacteria were consistently isolated from diseased tissues. Pathogenicity tests established that representative strains induced disease symptoms similar to those observed on maize plants in the field. Physiological and biochemical characterization using the API 20E and API 50CHE systems and 16S rRNA gene sequence analyses showed that the strains belonged to the genus Pantoea. The results of these tests also separated the strains into two groups. The first group, giving a positive reaction in the indole test, was similar to Pantoea ananatis. The second group of strains was indole negative and resembled P. agglomerans. The fluorescent amplified fragment length polymorphism (F-AFLP) genomic fingerprints generated by the indole-positive strains and P. ananatis reference strains were similar and clustered together in the dendrogram, confirming that the indole-positive bacteria causing brown stalk rot on maize were P. ananatis. The F-AFLP fingerprints produced by the indole-negative strains were distinctly different from those generated by P. ananatis, P. agglomerans, P. dispersa, P. citrea, P. stewartii subsp. stewartii, and P. stewartii subsp. indologenes. The results indicated that indole-negative bacteria causing brown stalk rot on maize might belong to a previously undescribed species of the genus Pantoea. This is the first report of a new disease on maize, brown stalk rot, caused by two bacterial species, P. ananatis and an undescribed Pantoea sp.

scholarly journals Qualitative Detection and Isolation of Bacteria from Surfaces of Canned Drinks Sold in Ugbor, Benin City

2018 ◽  
Vol 3 (2) ◽  
pp. 20-25
Author(s):  
Ogofure G. Abraham ◽  
Bello-Osagie O. Idowu ◽  
Aduba U. Barbara ◽  
Ighodaro E. Veadams ◽  
Emoghene O. Alexander
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Species ◽  
Qualitative Assessment ◽  
Public Health Importance ◽  
Isolation And Identification ◽  
Mannitol Salt Agar ◽  
Benin City ◽  
Diffusion Technique ◽  
Qualitative Detection ◽  
Mar Index

AbstractThe qualitative assessment of putative bacterial pathogens on the surfaces of canned drinks sold in Benin metropolis was evaluated in this study. Standard bacteriological culture-based techniques employing the use of selective and differential media (Oxoid) such asBacillus cereusagar, mannitol Salt agar,Pseudomonascetrimide agar, bile esculin agar and MacConkey agar were used for isolation and identification of bacteria from swabbed surfaces of canned drinks. Kirby-Bauer disc diffusion technique was used for antibacterial susceptibility testing. The multiple antibiotic resistance (MAR) index was deduced from the antibiogram characterization to evaluate the public health importance of the bacterial isolates. Refrigerated samples had 25% contamination while 75% were not contaminated and about 15.39% contamination was observed for non-refrigerated samples (stored in crates or cartons) compared to the counterpart 84.61%. The bacterial species includeStaphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillussp. andEnterococcussp. The bacteria were found to be sensitive to ciprofloxacin (92.5%) and gentamicin (90.1%) and least susceptible to cefixime (23.1%) and vancomycin (26.4%). They were found to be multi-resistant because they have an MAR index above the tolerable permissible limit (0.2) for common antibiotics usually used for their eradication. It is important to ensure that the surfaces of canned drinks must be rinsed with water before consumption.

scholarly journals Prevalence of Bacterial Vaginosis among Married Women in Kalar District, Iraqi Kurdistan Region

passer ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 194-199
Author(s):  
Saman Mohammed Mohammed- Amin
Keyword(s):  
Bacterial Vaginosis ◽  
Pathogenic Bacteria ◽  
Clinical Symptoms ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Married Women ◽  
P Value ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Isolation And Identification

Abstract Bacterial vaginosis (BV) is an inflammatory disease, caused by polymicrobial infection, including pathogenic bacteria which replace the vaginal normal flora and finally this replacement causes manifestations of several physiological and clinical symptoms among women within different ages. BV has become one of the main problems that make woman patients visit gynecological and obstetric consultant hospitals in most country. The present study is designed to determine the causative pathogen and the prevalence of bacterial vaginosis among married women patients in Kalar district. This cross-sectional study was performed from the beginning of March to the mid April-2021 among women who attended Obstetrics and Gynecological governmental hospital and out-patient clinics in Kalar City. Intra vaginal swabs have been collected in sterile Amies transport medium sticks and processed for isolation and identification of bacterial species depending on colony morphology, Gram’s stain and microbiological analysis protocols. Then socio-demographic and gynaecologic data were collected by questionnaire. Out of the 108 participant women who suffered from Gynecological diseases, 67(62.03%) of them exhibited bacterial vaginosis. From the 73 different isolated colonies, 18 bacterial species were identified; coagulase-negative staphylococci (CoNS) were the predominant cause of BV (32.84%), followed by E. coli (14.93%), Staphylococcus aureus (13.43%), Klebsiella pneumonia (8.96) and Micrococcus luteus (7.46%), while Proteus spp. and some uncommon bacteria display (1.49%) for each of them. The socio-demographic analysis between positive and negative woman patients revealed that the association between all studied risk factors and BV were statistically significant (P value < 0.05) except the age factor which was statistically non-significant meaning that the age was not associated with BV. In addition, the clinical symptom analysis showed that abnormal vaginal discharge, lower back pain, dysmenorrhea and strawberry were significantly associated with BV (P value < 0.05), while the rest of other factor did not exhibit statistically significant association.

scholarly journals Transmission of Novel Bacterial Pathogens through Pigs Transported from Myanmar to Mizoram

2021 ◽  
Author(s):  
C. Lalremruata ◽  
T.K. Dutta ◽  
P. Roychoudhury ◽  
Sanjeev Kumar ◽  
A. Sen ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Virulence Genes ◽  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Microbial Pathogens ◽  
Identification System ◽  
Bacterial Strains ◽  
Illegal Migration ◽  
Isolation And Identification ◽  
Specific Pcr

Background: Illegal migration of pigs/piglets from Myanmar to Mizoram is a common practice to meet the local demands. The migrated animals are suspected as potential carrier of various microbial pathogens. The present study was conducted on isolation, identification and molecular characterization of major bacterial pathogens (Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Haemophilus parasuis, Mycoplasma hyopneumoniae and Pasteurella multocida) in pigs illegally migrated from Myanmar to Mizoram. Methods: A total of 209 rectal swabs and 209 nasal swabs were collected from apparently healthy migrated pigs during October 2018 to April, 2019. All the samples were processed for PCR based detection of target bacterial species followed by isolation and identification by bacteriological techniques. The bacterial species were further confirmed by BD Phoenix automated bacterial identification system and selected virulence genes of the bacterial species were determined by specific PCR assay. Result: By species specific PCR, 110 samples were found to be positive for selected bacterial species, of which 20 (9.57%), 1 (0.478%), 86 (41.15%), 2 (0.956%) and 1 (0.478%) were A. pleuropneumoniae, B. bronchiseptica, H. parasuis, M. hyopneumoniae and P. multocida, respectively. A total of 52 bacterial strains were isolated and identified, of which 11, 1, 39 and 1 were A. pleuropneumoniae, B. bronchiseptica, H. parasuis, M. hyopneumoniae and P. multocida, respectively. Virulence genes were detected in A. pleuropneumoniae and H. parasuis isolates. Based upon the published literatures, this is the first ever report of isolation and identification of pathogenic A. pleuropneumoniae and H. parasuis in pigs in India.

scholarly journals Isolation and Identification of Phenol-Degrading Bacteria from Oil-Contaminated Sites

2021 ◽  
Vol 38 (1) ◽  
pp. 160-165
Author(s):  
Z.M. Usman ◽  
M.A. Said ◽  
F.A. Shehu ◽  
K. Abdussalam ◽  
T.M. Abdulrazak ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
System Analysis ◽  
Bacterial Species ◽  
Phenol Degradation ◽  
Contaminated Sites ◽  
Isolation And Identification ◽  
Selective Enrichment ◽  
Enrichment Technique ◽  
Degrading Bacteria ◽  
Cupriavidus Pauculus

This work is aimed at isolating and identifying phenol-degrading bacteria from oil-contaminated sites. Five soil samples from three auto-mechanic workshops within Katsina metropolis were collected. The samples were analyzed by selective enrichment technique, which resulted in the isolation of four bacterial species. The species were further subjected to the Vitek 2 compact microbiological system analysis. Cupriavidus pauculus, Pontoea spp, Proteus mirabilis 1 and Proteus mirabilis 2 were identified. Result from the present study showed that the bacteria could utilize phenol as their carbon source. Proteus mirabilis 1 and Proteus mirabilis 2 showed lower phenol degradation potential, under similar conditions. Cupriavidus pauculus and Pontoea sp. showed significant increases (p<0.05) in their optical densities. The optical density increment is strongly correlated with increase in colony forming units of the bacteria. This study further showed that the isolates could tolerate high phenol concentrations and may serve as strong putative isolates in bioremediation of phenol-contaminated sites.

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