scholarly journals Detection of clustered DNA damage in neuronal cells induced by ionizing radiation with different physical characteristics

2021 ◽  
Author(s):  
Regina A. Kozhina ◽  
Vladimir N. Chausov ◽  
Anfisa S. Filatova ◽  
Tatiana S. Hramco ◽  
Elizaveta V. Ilyina ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Neuronal Cells ◽  
Physical Characteristics ◽  
Clustered Dna Damage

scholarly journals Direct observation of damage clustering in irradiated DNA with atomic force microscopy

2019 ◽  
Vol 48 (3) ◽  
pp. e18-e18 ◽  
Author(s):  
Xu Xu ◽  
Toshiaki Nakano ◽  
Masataka Tsuda ◽  
Ryota Kanamoto ◽  
Ryoichi Hirayama ◽  
...  
Keyword(s):  
Dna Damage ◽  
Atomic Force Microscopy ◽  
Ionizing Radiation ◽  
Ion Beams ◽  
Dna Lesions ◽  
X Rays ◽  
Damage Site ◽  
Force Microscopy ◽  
Atomic Force ◽  
Clustered Dna Damage

Abstract Ionizing radiation produces clustered DNA damage that contains two or more lesions in 10–20 bp. It is believed that the complexity of clustered damage (i.e., the number of lesions per damage site) is related to the biological severity of ionizing radiation. However, only simple clustered damage containing two vicinal lesions has been demonstrated experimentally. Here we developed a novel method to analyze the complexity of clustered DNA damage. Plasmid DNA was irradiated with densely and sparsely ionizing Fe-ion beams and X-rays, respectively. Then, the resulting DNA lesions were labeled with biotin/streptavidin and observed with atomic force microscopy. Fe-ion beams produced complex clustered damage containing 2–4 lesions. Furthermore, they generated two or three clustered damage sites in a single plasmid molecule that resulted from the hit of a single track of Fe-ion beams. Conversely, X-rays produced relatively simple clustered damage. The present results provide the first experimental evidence for complex cluster damage.

scholarly journals Ionizing Radiation and Complex DNA Damage: From Prediction to Detection Challenges and Biological Significance

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1789 ◽  
Author(s):  
Ifigeneia V. Mavragani ◽  
Zacharenia Nikitaki ◽  
Spyridon A. Kalospyros ◽  
Alexandros G. Georgakilas
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Biological Significance ◽  
Biological Responses ◽  
Literature Evidence ◽  
Rate Type ◽  
Clustered Dna Damage

Biological responses to ionizing radiation (IR) have been studied for many years, generally showing the dependence of these responses on the quality of radiation, i.e., the radiation particle type and energy, types of DNA damage, dose and dose rate, type of cells, etc. There is accumulating evidence on the pivotal role of complex (clustered) DNA damage towards the determination of the final biological or even clinical outcome after exposure to IR. In this review, we provide literature evidence about the significant role of damage clustering and advancements that have been made through the years in its detection and prediction using Monte Carlo (MC) simulations. We conclude that in the future, emphasis should be given to a better understanding of the mechanistic links between the induction of complex DNA damage, its processing, and systemic effects at the organism level, like genomic instability and immune responses.

scholarly journals The Yield, Processing, and Biological Consequences of Clustered DNA Damage Induced by Ionizing Radiation

2009 ◽  
Vol 50 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Naoya SHIKAZONO ◽  
Miho NOGUCHI ◽  
Kentaro FUJII ◽  
Ayumi URUSHIBARA ◽  
Akinari YOKOYA
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Clustered Dna Damage

scholarly journals A Small Compound KJ-28d Enhances the Sensitivity of Non-Small Cell Lung Cancer to Radio- and Chemotherapy

2019 ◽  
Vol 20 (23) ◽  
pp. 6026
Author(s):  
Hwani Ryu ◽  
Hyo Jeong Kim ◽  
Jie-Young Song ◽  
Sang-Gu Hwang ◽  
Jae-Sung Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Damage ◽  
Ionizing Radiation ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Therapeutic Agent ◽  
Apoptotic Cell ◽  
Combined Treatment ◽  
Small Cell ◽  
Small Cell Lung

We previously reported on a poly (ADP-ribose) polymerase (PARP) 1/2 inhibitor N-(3-(hydroxycarbamoyl)phenyl)carboxamide (designated KJ-28d), which increased the death of human ovarian cancer BRCA1-deficient SNU-251 cells. In the present study, we further investigated the antitumor activities of KJ-28d in BRCA-proficient non-small cell lung cancer (NSCLC) cells to expand the use of PARP inhibitors. KJ-28d significantly inhibited the growth of NSCLC cells in vitro and in vivo, and induced DNA damage and reactive oxygen species in A549 and H1299 cells. Combined treatment with KJ-28d and ionizing radiation led to increased DNA damage responses in A549 and H1299 cells compared to KJ-28d or ionizing radiation alone, resulting in apoptotic cell death. Moreover, the combination of KJ-28d plus a DNA-damaging therapeutic agent (carboplatin, cisplatin, paclitaxel, or doxorubicin) synergistically inhibited cell proliferation, compared to either drug alone. Taken together, the findings demonstrate the potential of KJ-28d as an effective anti-cancer therapeutic agent for BRCA-deficient and -proficient cancer cells. KJ-28d might have potential as an adjuvant when used in combination with radiotherapy or DNA-damaging agents, pending further investigations.

Characterization of DNA damage-induced cellular senescence by ionizing radiation in endothelial cells

2013 ◽  
Vol 90 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Kwang Seok Kim ◽  
Jung Eun Kim ◽  
Kyu Jin Choi ◽  
Sangwoo Bae ◽  
Dong Ho Kim
Keyword(s):  
Dna Damage ◽  
Endothelial Cells ◽  
Ionizing Radiation ◽  
Cellular Senescence

Interaction of poly(ADP-ribose) polymerase 1 with apurinic/apyrimidinic sites within clustered DNA damage

2011 ◽  
Vol 76 (1) ◽  
pp. 147-156 ◽  
Author(s):  
M. M. Kutuzov ◽  
E. S. Ilina ◽  
M. V. Sukhanova ◽  
I. A. Pyshnaya ◽  
D. V. Pyshnyi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Clustered Dna Damage
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