scholarly journals Wide-field magneto-optical microscope to access quantitative magnetization dynamics with femtosecond temporal and sub-micrometer spatial resolution

2021 ◽  
Vol 130 (8) ◽  
pp. 083905
Author(s):  
F. Steinbach ◽  
D. Schick ◽  
C. von Korff Schmising ◽  
K. Yao ◽  
M. Borchert ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Optical Microscope ◽  
Wide Field ◽  
Magnetization Dynamics

A practical wide-field Raman imaging method with high spectral and spatial resolution

2018 ◽  
Vol 89 (8) ◽  
pp. 083103 ◽  
Author(s):  
Haibo Li ◽  
Wenhua Luo ◽  
Gan Li ◽  
Guangfeng Zhang ◽  
Pengcheng Zhang ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Raman Imaging ◽  
Imaging Method ◽  
Wide Field

scholarly journals Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination

2019 ◽  
Vol 5 (3) ◽  
pp. eaav5335 ◽  
Author(s):  
Yan Kuai ◽  
Junxue Chen ◽  
Xi Tang ◽  
Yifeng Xiang ◽  
Fengya Lu ◽  
...  
Keyword(s):  
Free Surface ◽  
Spatial Resolution ◽  
High Surface ◽  
Metal Films ◽  
Wide Field ◽  
Label Free ◽  
Dielectric Substrates ◽  
Surface Sensitivity ◽  
Order Of Magnitude ◽  
Culture Growth

Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology.

scholarly journals A wide field-of-view imaging DOAS instrument for two-dimensional trace gas mapping from aircraft

2015 ◽  
Vol 8 (12) ◽  
pp. 5113-5131 ◽  
Author(s):  
A. Schönhardt ◽  
P. Altube ◽  
K. Gerilowski ◽  
S. Krautwurst ◽  
J. Hartmann ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Point Sources ◽  
West Germany ◽  
Field Of View ◽  
Trace Gas ◽  
Optical Absorption Spectroscopy ◽  
Small Scale ◽  
Wide Field ◽  
Flight Altitude ◽  
Good Imaging

Abstract. The Airborne imaging differential optical absorption spectroscopy (DOAS) instrument for Measurements of Atmospheric Pollution (AirMAP) has been developed for the purpose of trace gas measurements and pollution mapping. The instrument has been characterized and successfully operated from aircraft. Nitrogen dioxide (NO2) columns were retrieved from the AirMAP observations. A major benefit of the push-broom imaging instrument is the spatially continuous, gap-free measurement sequence independent of flight altitude, a valuable characteristic for mapping purposes. This is made possible by the use of a charge coupled device (CCD) frame-transfer detector. A broad field of view across track of around 48° is achieved with wide-angle entrance optics. This leads to a swath width of about the same size as the flight altitude. The use of fibre coupled light intake optics with sorted light fibres allows flexible instrument positioning within the aircraft and retains the very good imaging capabilities. The measurements yield ground spatial resolutions below 100 m depending on flight altitude. The number of viewing directions is chosen from a maximum of 35 individual viewing directions (lines of sight, LOS) represented by 35 individual fibres. The selection is adapted to each situation by averaging according to signal-to-noise or spatial resolution requirements. Observations at 30 m spatial resolution are obtained when flying at 1000 m altitude and making use of all 35 viewing directions. This makes the instrument a suitable tool for mapping trace gas point sources and small-scale variability. The position and aircraft attitude are taken into account for accurate spatial mapping using the Attitude and Heading Reference System of the aircraft. A first demonstration mission using AirMAP was undertaken in June 2011. AirMAP was operated on the AWI Polar-5 aircraft in the framework of the AIRMETH-2011 campaign. During a flight above a medium-sized coal-fired power plant in north-west Germany, AirMAP clearly detected the emission plume downwind from the exhaust stack, with NO2 vertical columns around 2 × 1016 molecules cm−2 in the plume centre. NOx emissions estimated from the AirMAP observations are consistent with reports in the European Pollutant Release and Transfer Register. Strong spatial gradients and variability in NO2 amounts across and along flight direction are observed, and small-scale enhancements of NO2 above a motorway are detected.

scholarly journals Optical nanoscopy

2020 ◽  
Vol 43 (8) ◽  
pp. 385-455
Author(s):  
A. Diaspro ◽  
P. Bianchini
Keyword(s):  
Artificial Intelligence ◽  
Spatial Resolution ◽  
Scanning Probe Microscopy ◽  
Molecular Level ◽  
Super Resolution ◽  
Optical Microscope ◽  
Scanning Probe ◽  
Living Systems ◽  
Starting Point ◽  
Basic Concepts

Abstract This article deals with the developments of optical microscopy towards nanoscopy. Basic concepts of the methods implemented to obtain spatial super-resolution are described, along with concepts related to the study of biological systems at the molecular level. Fluorescence as a mechanism of contrast and spatial resolution will be the starting point to developing a multi-messenger optical microscope tunable down to the nanoscale in living systems. Moreover, the integration of optical nanoscopy with scanning probe microscopy and the charming possibility of using artificial intelligence approaches will be shortly outlined.

scholarly journals AFM and Fluorescence Microscopy of Single Cells with Simultaneous Mechanical Stimulation via Electrically Stretchable Substrates

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4131
Author(s):  
Natalia Becerra ◽  
Barbara Salis ◽  
Mariateresa Tedesco ◽  
Susana Moreno Flores ◽  
Pasquale Vena ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Spatial Resolution ◽  
Single Cells ◽  
Optical Microscope ◽  
Average Cell ◽  
Atomic Force ◽  
Cell Stretcher

We have developed a novel experimental set-up that simultaneously, (i) applies static and dynamic deformations to adherent cells in culture, (ii) allows the visualization of cells under fluorescence microscopy, and (iii) allows atomic force microscopy nanoindentation measurements of the mechanical properties of the cells. The cell stretcher device relies on a dielectric elastomer film that can be electro-actuated and acts as the cell culture substrate. The shape and position of the electrodes actuating the film can be controlled by design in order to obtain specific deformations across the cell culture chamber. By using optical markers we characterized the strain fields under different electrode configurations and applied potentials. The combined setup, which includes the cell stretcher device, an atomic force microscope, and an inverted optical microscope, can assess in situ and with sub-micron spatial resolution single cell topography and elasticity, as well as ion fluxes, during the application of static deformations. Proof of performance on fibroblasts shows a reproducible increase in the average cell elastic modulus as a response to applied uniaxial stretch of just 4%. Additionally, high resolution topography and elasticity maps on a single fibroblast can be acquired while the cell is deformed, providing evidence of long-term instrumental stability. This study provides a proof-of-concept of a novel platform that allows in situ and real time investigation of single cell mechano-transduction phenomena with sub-cellular spatial resolution.

High-speed UV imaging of elves and lightning from space: first simultaneous detections from the Mini-EUSO and ASIM instruments

2020 ◽  
Author(s):  
Matteo Battisti ◽  
Enrico Arnone ◽  
Mario Bertaina ◽  
Marco Casolino ◽  
Olivier Chanrion ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
High Speed ◽  
Multispectral Imaging ◽  
Gamma Ray ◽  
Single Photon ◽  
Uv Light ◽  
Photon Counting ◽  
Space Station ◽  
Wide Field ◽  
First Time

<p>The search for the physical mechanisms of lightning, transient luminous events and terrestrial gamma-ray flashes is receiving an extraordinary support by new space observations that have recently become available. Next to lightning detectors on geostationary satellites, new low orbit experiments are giving an unprecedented insight in the very source of these processes. Looking at the physics behind these new observations requires however to have a variety of different instruments covering the same event, and this is proving extremely challenging. Here, we present observations of UV emissions of elves and lightning taken for the first time simultaneously from the two instruments Mini-EUSO and ASIM operating on the international space station. Mini-EUSO was designed to perform observations of the UV-light night emission from Earth. It is a wide field of view telescope (44°x44° square FOV) installed for the first time on October 2019 inside the Zvezda Module of the ISS, looking nadir through a UV transparent window. Its optical system consists of two Fresnel lenses for light collection. The light is focused onto an array of 36 multi-anode photomultiplier tubes (MAPMT), for a total of 2304 pixels. Each pixel has a footprint on ground of ~5.5 km. The instrument is capable of single-photon counting on three different timescales: a 2.5 microsecond (D1) and a 320 microsecond (D2) timescale with a dedicated trigger system, and a 40.96ms timescale (D3) used to produce a continuous monitoring of the UV emission from the Earth. ASIM is an experiment dedicated to lightning and atmospheric processes. Its Modular Multispectral Imaging Array (MMIA) is made of an array of 3 high speed photometers probing different wavelength sampling at rates up to 100 kHz, and 2 Electron Multiplication Charge Coupled Devices (EM-CCDs) with a sub-km spatial resolution with an 80° FOV and recording up to 12 frames per second. Mini-EUSO detected several bright atmospheric events like lightning and elves, with a few km spatial resolution and different time resolutions, probing therefore different stages of the electromagnetic phenomena. Observations from Mini-EUSO were simultaneously captured by ASIM instruments, allowing for the first time to compare and complement the capabilities of the two instruments with a time inter-calibration based on unambiguous series of lightning detections.</p>

scholarly journals Simultaneous multiview capture and fusion improves spatial resolution in wide-field and light-sheet microscopy

Optica ◽  
2016 ◽  
Vol 3 (8) ◽  
pp. 897 ◽  
Author(s):  
Yicong Wu ◽  
Panagiotis Chandris ◽  
Peter W. Winter ◽  
Edward Y. Kim ◽  
Valentin Jaumouillé ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Light Sheet ◽  
Wide Field ◽  
Light Sheet Microscopy

scholarly journals Simultaneous imaging of magnetic field and temperature using a wide-field quantum diamond microscope

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yulei Chen ◽  
Zhonghao Li ◽  
Hao Guo ◽  
Dajin Wu ◽  
Jun Tang
Keyword(s):  
Magnetic Field ◽  
Spatial Resolution ◽  
Imaging Technique ◽  
Field Of View ◽  
Nitrogen Vacancy ◽  
Wide Field ◽  
Simultaneous Imaging ◽  
Quantum Sensing ◽  
Nitrogen Vacancy Centers

AbstractQuantum sensing based on nitrogen-vacancy centers in diamond has shown excellent properties. Combined with the imaging technique, it shows exciting practicability. Here, we demonstrate the simultaneously imaging technique of magnetic field and temperature using a wide-field quantum diamond microscope. We describe the operating principles of the diamond microscope and report its sensitivity (magnetic field ${\sim}1.8~\mu \mbox{T/Hz}^{1/2}$ ∼ 1.8 μ T/Hz 1 / 2 and temperature ${\sim}0.4~\mbox{K/Hz}^{1/2}$ ∼ 0.4 K/Hz 1 / 2 ), spatial resolution (1.3 μm), and field of view ($400 \times 300~\mu \mbox{m}^{2}$ 400 × 300 μ m 2 ). Finally, we use the microscope to obtain images of an integrated cell heater and a PCB, demonstrating its ability in the application of magnetic field and temperature simultaneously imaging at wide-field.

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