Bidomain modeling of electrical and mechanical properties of cardiac tissue

2021 ◽  
Vol 2 (4) ◽  
pp. 041301
Author(s):  
Bradley J. Roth
Keyword(s):  
Mechanical Properties ◽  
Cardiac Tissue

scholarly journals Investigation of microstructure evolution and mechanical properties in cardiac tissue

2020 ◽  
Vol 23 (sup1) ◽  
pp. S297-S299
Author(s):  
N. Tueni ◽  
J. Vizet ◽  
M. Genet ◽  
A. Pierangelo ◽  
J. M. Allain
Keyword(s):  
Mechanical Properties ◽  
Microstructure Evolution ◽  
Cardiac Tissue

scholarly journals Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

2019 ◽  
Vol 30 (7) ◽  
pp. 1908612 ◽  
Author(s):  
Kaveh Roshanbinfar ◽  
Lena Vogt ◽  
Florian Ruther ◽  
Judith A. Roether ◽  
Aldo R. Boccaccini ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

Effects of BDM, [Ca2+]o, and temperature on the dynamic stiffness of quiescent cardiac trabeculae from rat

2005 ◽  
Vol 288 (4) ◽  
pp. H1662-H1667 ◽  
Author(s):  
R. S. Kirton ◽  
A. J. Taberner ◽  
P. M. F. Nielsen ◽  
A. A. Young ◽  
D. S. Loiselle
Keyword(s):  
Mechanical Properties ◽  
Cardiac Tissue ◽  
Dynamic Stiffness ◽  
Low Frequency ◽  
Muscle Length ◽  
Small Degree ◽  
Experimental Conditions ◽  
Cross Bridge ◽  
Intracellular Ca ◽  
Increasing Temperature

Studies of the passive mechanical properties of cardiac tissue have traditionally been conducted at subphysiological temperatures and various concentrations of extracellular Ca2+ ([Ca2+]o). More recently, the negative inotropic agent 2,3-butanedione monoxime (BDM) has been used. However, there remains a lack of data regarding the influence of temperature, Ca2+, and BDM on the passive mechanical properties of cardiac tissue. We have used the dynamic stiffness technique, a sensitive measurement of cross-bridge activity, in which minute (∼0.2% of muscle length) sinusoidal perturbations are applied at various frequencies (0.2–100 Hz) to quiescent, viable right ventricular rat trabeculae at two temperatures (20°C and 26°C) and at two [Ca2+]o (0.5 and 1.25 mM) in the presence and absence of BDM (20 mM). The stiffness spectra (amplitude and phase) were sensitive to temperature and [Ca2+]o in the absence of BDM but insensitive in the presence of BDM. From the index of cross-bridge cycling (the ratio of high- to low-frequency stiffness amplitude), we infer that BDM inhibits a small degree of spontaneous sarcomere activity, thereby allowing the true passive properties of trabeculae to be determined. In the absence of BDM, the extent of spontaneous sarcomere activity decreases with increasing temperature. We caution that the measured mechanical properties of passive cardiac tissue are critically dependent on the experimental conditions under which they are measured. Experiments must be performed at sufficiently high temperatures (>25°C) to ensure a low resting concentration of intracellular Ca2+ or in the presence of an inhibitor of cross-bridge cycling.

Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering

2007 ◽  
Vol 3 (4) ◽  
pp. 457-462 ◽  
Author(s):  
L HIDALGOBASTIDA ◽  
J BARRY ◽  
N EVERITT ◽  
F ROSE ◽  
L BUTTERY ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Adhesion ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering

scholarly journals Development of Custom Wall-Less Cardiovascular Flow Phantoms with Tissue-Mimicking Gel

2021 ◽  
Author(s):  
Megan E. Laughlin ◽  
Sam E. Stephens ◽  
Jamie A. Hestekin ◽  
Morten O. Jensen
Keyword(s):  
Mechanical Properties ◽  
Continuous Flow ◽  
Cardiac Tissue ◽  
Flow Imaging ◽  
Straight Channel ◽  
Young’S Moduli ◽  
Flow Phantom ◽  
Cardiovascular Flow ◽  
3D Printed

Abstract Purpose Flow phantoms are used in experimental settings to aid in the simulation of blood flow. Custom geometries are available, but current phantom materials present issues with degradability and/or mimicking the mechanical properties of human tissue. In this study, a method of fabricating custom wall-less flow phantoms from a tissue-mimicking gel using 3D printed inserts is developed. Methods A 3D blood vessel geometry example of a bifurcated artery model was 3D printed in polyvinyl alcohol, embedded in tissue-mimicking gel, and subsequently dissolved to create a phantom. Uniaxial compression testing was performed to determine the Young’s moduli of the five gel types. Angle-independent, ultrasound-based imaging modalities, Vector Flow Imaging (VFI) and Blood Speckle Imaging (BSI), were utilized for flow visualization of a straight channel phantom. Results A wall-less phantom of the bifurcated artery was fabricated with minimal bubbles and continuous flow demonstrated. Additionally, flow was visualized through a straight channel phantom by VFI and BSI. The available gel types are suitable for mimicking a variety of tissue types, including cardiac tissue and blood vessels. Conclusion Custom, tissue-mimicking flow phantoms can be fabricated using the developed methodology and have potential for use in a variety of applications, including ultrasound-based imaging methods. This is the first reported use of BSI with an in vitro flow phantom.

scholarly journals Nanomechanical Properties of Engineered Cardiomyocytes Under Electrical Stimulation

2021 ◽  
Author(s):  
Lia Pailino ◽  
Lihua Lou ◽  
Alberto Sesena Rubfiaro ◽  
Jin He ◽  
Arvind Agarwal
Keyword(s):  
Mechanical Properties ◽  
Electrical Stimulation ◽  
Elastic Modulus ◽  
Cardiac Tissue ◽  
Control Mode ◽  
Adult Cardiomyocytes ◽  
Fetal Cardiomyocytes ◽  
Induced Pluripotent ◽  
Fluid Cell

Engineered cardiomyocytes made of human-induced pluripotent stem cells (iPSC) present phenotypical characteristics similar to human fetal cardiomyocytes. There are different factors that are essential for engineered cardiomyocytes to be functional, one of them being that their mechanical properties must mimic those of adult cardiomyocytes. Techniques, such as electrical stimulation, have been used to improve the extracellular matrix's alignment and organization and improve the intracellular environment. Therefore, electrical stimulation could potentially be used to enhance the mechanical properties of engineered cardiac tissue. The goal of this study is to establish the effects of electrical stimulation on the elastic modulus of engineered cardiac tissue. Nanoindentation tests were performed on engineered cardiomyocyte constructs under seven days of electrical stimulation and engineered cardiomyocyte constructs without electrical stimulation. The tests were conducted using BioSoft™ In-Situ Indenter through displacement control mode with a 50 µm conospherical diamond fluid cell probe. The Hertzian fit model was used to analyze the data and obtain the elastic modulus for each construct. This study demonstrated that electrically stimulated cardiomyocytes (6.98 ± 0.04 kPa) present higher elastic modulus than cardiomyocytes without electrical stimulation (4.96 ± 0.29 kPa) at day 7 of maturation. These results confirm that electrical stimulation improves the maturation of cardiomyocytes. Through this study, an efficient nanoindentation method is demonstrated for engineered cardiomyocyte tissues, capable of capturing the nanomechanical differences between electrically stimulated and non-electrically stimulated cardiomyocytes.

Abstract 136: Gel Stiffness-tuned, Nanostructure-templated Alignment and Maturation of Cardiomyocytes For Cardiac Tissue Engineering

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Eduard Sleep ◽  
Jason R Mantei ◽  
Mark T McClendon ◽  
Samuel I Stupp
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Survival ◽  
Cardiac Tissue ◽  
Gel Properties ◽  
Electrophysiological Properties ◽  
Functional Maturation ◽  
And Function ◽  
First Time ◽  
Over Time

Objective: To investigate how the supramolecular structure and mechanical properties of hydrogels made of chemically-defined peptide amphiphiles (PAs) could influence i) the alignment of cardiomyocytes seeded in them and ii) the functional maturation of the cell construct. Methods: We generated a series of PAs with different peptide sequences that allowed us to make PA gels with stiffness values ranging three orders of magnitude. The nature of the gelation process provides these gels with same-direction oriented nanofibers along an elongated PA gel. We seeded these PA gels with either HL-1 cardiomyocytes or iPSC-derived cardiomyocytes and cultured them for up to two weeks. We then measured cell survival, proliferation and alignment over time and also the electrophysiological properties of the cell construct as a whole. Results: We found that cardiomyocytes responded to the alignment of the nanofibers in the gel by aligning to them and that this process can be tuned by changing the stiffness of the PA gel. This in turn influenced the investigated electrophysiological parameters of the cell construct suggesting a functional maturation induced by the PA gel properties. Conclusions: We believe this study shows for the first time that the nanostructural and mechanical properties of hydrogels can be exploited to influence the maturation of cardiomyocytes/cell construct. This can have important implications for strategies that aim to use ESC- or iPSC-derived cardiomyocytes in tissue engineering, as there is a need for their proper maturation and function.

Sign in / Sign up

Export Citation Format

Share Document