Application and composition of sawdust, grass straw, rice husk, and rice bran for white oyster mushroom (Pleurotus ostreatus Jacq.) growth media using hydrated lime sterilization

2021 ◽  
Author(s):  
A. Hendrika ◽  
A. F. Soffan ◽  
M. G. Althaf ◽  
S. P. Amanda ◽  
R. M. Fani ◽  
...  
Keyword(s):  
Rice Husk ◽  
Rice Bran ◽  
Pleurotus Ostreatus ◽  
Hydrated Lime ◽  
Oyster Mushroom ◽  
Growth Media

scholarly journals Maize Residue as a Viable Substrate for Farm Scale Cultivation of Oyster Mushroom(Pleurotus ostreatus)

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Abena O. Adjapong ◽  
Kwame D. Ansah ◽  
Faustina Angfaarabung ◽  
Henry O. Sintim
Keyword(s):  
Rice Bran ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Biological Efficiency ◽  
Growth Media ◽  
Organic Substrates ◽  
Mushroom Cultivation ◽  
Fruiting Bodies ◽  
Growing Media ◽  
Maize Cob

In the search for alternatives to sawdust as growing media in commercial mushroom cultivation, three organic substrates obtainable as crop residue, maize husk, maize cob, and maize stalk, with each being supplemented with rice bran, were evaluated as growth media for the oyster mushroom,Pleurotus ostreatus(Kummer). For the tested alternatives to sawdust, the harvested weight of fruiting bodies that sprouted on a kilogram maize husk media per crop (32.99 g) was the highest. Sawdust media supported significantly (P<0.001) heavier fruiting bodies (42.18) than the maize residues. The peak mushroom harvests for the various substrates were obtained between the first and seventh fruiting body flushes. The biological efficiency of the substrates, which measured usable nutrients indicated that maize stalk supplemented with rice bran, was 39% compared to that of the sawdust media (60%). The maize husk media and the maize cob media had biological efficiencies of 32% and 9.5%, respectively. These results indicate that two of the tested growing media (maize stalk or husk) produced mushrooms with yield characteristics that were comparable to the well-used sawdust in the cultivation of oyster mushrooms. The environmental and economic parameters involved in the use and carting of sawdust make these on-farm crop residues a viable alternative for mushroom cultivation in especially nonforest zones of Ghana.

scholarly journals Pola aktivitas enzim ligninolitik Pleurotus ostreatus pada limbah sludge pabrik kertas Activity pattern of ligninolytic enzyme of Pleurotus ostreatus in sludge waste of paper factory

2016 ◽  
Vol 76 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
. TRI-PANJI
Keyword(s):  
Rice Bran ◽  
Growth Phase ◽  
Fruiting Body ◽  
Activity Pattern ◽  
Vegetative Growth ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Oyster Mushroom ◽  
Sludge Waste

Summary Sludge is a solid waste abundantly available on paper factory that is economically unutilized and tends to pollute environment. This waste can be used as growth media for oyster mushroom (Pleurotus ostreatus) as edible mushroom and ligninolytic enzymes production as well. A research has been conducted to study the activity pattern of ligninolytic enzymes of oyster mushroom grown on the sludge waste of recycle paper factory. Six treatments were examinated consisted of three media combinations (sawdust, sludge, sludge mixed with sawdust), with and without supplementing with rice bran, lime, and gypsum, and two mushroom strains Bogor oyster mushroom (JTB) and China Taipei oyster mushroom (JTT). Monitoring of ligninolytic enzyme activity consisting of laccase, mangan peroxidase (Mn-P) and lignin peroxidase (Li-P),  was subsequently regularly started since inoculation, at vegetative phase (four and six weeks), primordial formation, phase of fruiting body formation, and two weeks after formation of fruiting body. Each treatment was repeated three times, so that 216 bag logs of oyster mushroom cultures were performed. The results showed that laccase, Mn-P, and Li-P activities could be observed on sludge or mixture of sludge+sawdust media inoculated with P. ostreatus. Generally, the highest activity of ligninolytic enzymes especially for laccase and MnP were observed at the first vegetative growth phase i.e. before emerging primordial of fruiting body (1.697 & 2.113 U/mL, 4.394 & 2.314 U/mL  respectively for JTB and JTT laccase and JTB & JTT Mn-P). The highest Li-P activity was affected by the kind of media and strain of inoculum. In sludge medium, the highest Li-P activity was observed in  vegetative growth phase (2.706 & 4.014 U/mL respectively for JTB and JTT) while in a mixture of sludge + sawdust the highest activity of that enzyme was observed in primordial phase of growth (2.509 & 1.9 U/mL respectively for JTB and JTT). Addition of supplement to the sludge increased ligninolytic activity, while laccase activity of sludge was suggested could be more enhanced by mixing the sludge with sawdust and enrich with rice bran, gypsum and lime. Ringkasan                                                Sludge merupakan limbah padat yang tersedia melimpah di pabrik kertas dan belum dimanfaatkan secara ekonomis sehingga berpotensi mencemari lingkungan. Limbah ini dapat dimanfaatkan sebagai medium tumbuh jamur konsumsi seperti jamur tiram (Pleurotus ostreatus) dan penghasil enzim ligninolitik. Penelitian dilakukan untuk mempelajari pola aktivitas enzim ligninolitik jamur tiram pada limbah sludge pabrik kertas selama fase vegetatif sampai setelah fase generatif. Enam perlakuan yang diuji berupa tiga kombinasi komposisi medium (serbuk gergaji, sludge, campuran sludge dan serbuk gergaji), dengan dan tanpa pengayaan, yaitu penambahan dedak, kapur, dan gipsum,  serta dua strain jamur tiram Bogor (JTB) dan jamur tiram China Taipei (JTT). Pengamatan aktivitas enzim ligninolitik meliputi lakase, mangan peroksidase (Mn-P) dan lignin peroksidase  (Li-P) dilakukan sejak saat inokulasi, pada fase vegetatif (empat dan enam minggu), pada saat pembentukan primordia, fase tubuh buah, dan dua minggu setelah pembentukan tubuh buah. Masing-masing perlakuan diulang tiga kali sehingga terdapat 216 bag log jamur tiram. Hasil penelitian menunjukkan bahwa aktivitas ligninolitik dijumpai pada medium sludge dan campuran sludge+serbuk gergaji yang diino-kulasi P. ostreatus. Aktivitas enzim ligninolitik tertinggi khususnya lakase dan MnP teramati pada fase pertumbuhan vegetatif pertama yaitu sebelum terbentuknya primordia (1,697 & 2,113 U/mL, 4,394 & 2,314 U/mL  masing-masing untuk lakase JTB dan JTT dan MnP  JTB & JTT). Aktivitas LiP tertinggi dipengaruhi oleh jenis medium dan strain inokulum. Pada medium sludge, aktivitas LiP tertinggi dijumpai pada fase vegetatif (2,706 & 4,014 U/ml masing-masing untuk JTB dan JTT) sedangkan pada medium campuran sludge+serbuk gergaji, aktivitas enzim  ter-tinggi dijumpai  pada fase primordia (2,509 & 1,9 U/ml berturut-turut untuk JTB dan JTT). Pengayaan sludge meningkatkan aktivitas ligninolitik, sedangkan aktivitas lakase pada sludge diduga dapat lebih ditingkatkan dengan menambahkan serbuk gergaji disertai pengayaan berupa gipsum, dedak, dan kapur.

scholarly journals KAJIAN PENGGUNAAN SERASAH DAUN KAKAO UNTUK SUBTITUSI SERBUK GERGAJI DAN DEDAK PADI SEBAGAI MEDIA TANAM JAMUR TIRAM PUTIH (Pleurotus ostreatus)

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 41
Author(s):  
Abd. Rahman Razak ◽  
Susanti Susanti ◽  
Nurhaeni Nurhaeni ◽  
Muh. Alwi
Keyword(s):  
Leaf Litter ◽  
Rice Bran ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Fruiting Bodies ◽  
Treatment Comparison ◽  
Treatment Standard ◽  
Nutrient Value ◽  
Growing Medium ◽  
Oyster Mushrooms

Has done research on the study of the use of cocoa leaf litter for substitution sawdust and rice bran as a growing medium white oyster mushroom (Pleurotus ostreatus). This study aims to determine the ability of the cocoa leaf litter in substitute sawdust and rice bran as oyster mushroom media and to determine the nutrient value of the conversion of media into fruiting bodies of oyster mushrooms. Treatment comparison of leaf litter cocoa (SDK), sawdust (SG) and rice bran (DP) there are seven various ie first treatment / standard (SDK: 0 kg, SG: 5 kg, DP: 3 kg), second (SDK: 1 , 5 kg, SG: 5 kg, DP: 1.5 kg), third (SDK: 3 kg, SG: 5 kg, DP: 0 kg), fourth (SDK: 2.5 kg, SG: 2.5 kg DP: 3 kg), fifth (SDK: 5 kg, SG: 3 kg, DP: 0 kg), sixth (SDK: 5 kg, SG: 1.5 kg, DP: 1.5 kg), and seventh (SDK: 5 kg, SG: 0 kg, DP: 3 kg). The results showed that both treatments are best treated with a total weight of 181.122 g fruit.Keywords: cocoa leaf litter, white oyster mushroom

scholarly journals Investigation on Reducing Chromium Quantity in Chromium Containing Wastes from Leather Industry Using Oyster Mushroom (Pleurotus ostreatus)

2020 ◽  
Vol 115 (1) ◽  
pp. 3-9
Author(s):  
Eser Eke Bayramoğlu ◽  
Anıl Özçelik ◽  
Mehmet Çetin ◽  
Erkan Eren
Keyword(s):  
Environmental Pollution ◽  
Growth Medium ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Growth Media ◽  
Leather Industry ◽  
Icp Ms ◽  
Blue Phase ◽  
Oyster Mushrooms

In the leather industry, the wastes after the wet blue phase, which are created by the shaving process, are one of the substances that cause environmental pollution. Most of the time, these wastes are buried and may, under some circumstanses, cause serious environmental pollution problems. In this study, the chromium in wet blue shaved waste is to be minimized by using oyster mushrooms (Pleurotus ostreatus). Wet blue shaved wastes were mixed with 0.5%, 1%, 1.5% and 2% doses into the growth medium. After the oyster mushroom growth, the consumption of chromium from the growth media and uptaken by the mushrooms were investigated with  Ege University Argefar Lab.’s House Method using a ICP-MS. 

scholarly journals Pola aktivitas enzim ligninolitik Pleurotus ostreatus pada limbah sludge pabrik kertas Activity pattern of ligninolytic enzyme of Pleurotus ostreatus in sludge waste of paper factory

2016 ◽  
Vol 76 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
. TRI-PANJI
Keyword(s):  
Rice Bran ◽  
Growth Phase ◽  
Fruiting Body ◽  
Activity Pattern ◽  
Vegetative Growth ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Oyster Mushroom ◽  
Sludge Waste

Summary Sludge is a solid waste abundantly available on paper factory that is economically unutilized and tends to pollute environment. This waste can be used as growth media for oyster mushroom (Pleurotus ostreatus) as edible mushroom and ligninolytic enzymes production as well. A research has been conducted to study the activity pattern of ligninolytic enzymes of oyster mushroom grown on the sludge waste of recycle paper factory. Six treatments were examinated consisted of three media combinations (sawdust, sludge, sludge mixed with sawdust), with and without supplementing with rice bran, lime, and gypsum, and two mushroom strains Bogor oyster mushroom (JTB) and China Taipei oyster mushroom (JTT). Monitoring of ligninolytic enzyme activity consisting of laccase, mangan peroxidase (Mn-P) and lignin peroxidase (Li-P),  was subsequently regularly started since inoculation, at vegetative phase (four and six weeks), primordial formation, phase of fruiting body formation, and two weeks after formation of fruiting body. Each treatment was repeated three times, so that 216 bag logs of oyster mushroom cultures were performed. The results showed that laccase, Mn-P, and Li-P activities could be observed on sludge or mixture of sludge+sawdust media inoculated with P. ostreatus. Generally, the highest activity of ligninolytic enzymes especially for laccase and MnP were observed at the first vegetative growth phase i.e. before emerging primordial of fruiting body (1.697 & 2.113 U/mL, 4.394 & 2.314 U/mL  respectively for JTB and JTT laccase and JTB & JTT Mn-P). The highest Li-P activity was affected by the kind of media and strain of inoculum. In sludge medium, the highest Li-P activity was observed in  vegetative growth phase (2.706 & 4.014 U/mL respectively for JTB and JTT) while in a mixture of sludge + sawdust the highest activity of that enzyme was observed in primordial phase of growth (2.509 & 1.9 U/mL respectively for JTB and JTT). Addition of supplement to the sludge increased ligninolytic activity, while laccase activity of sludge was suggested could be more enhanced by mixing the sludge with sawdust and enrich with rice bran, gypsum and lime. Ringkasan                                                Sludge merupakan limbah padat yang tersedia melimpah di pabrik kertas dan belum dimanfaatkan secara ekonomis sehingga berpotensi mencemari lingkungan. Limbah ini dapat dimanfaatkan sebagai medium tumbuh jamur konsumsi seperti jamur tiram (Pleurotus ostreatus) dan penghasil enzim ligninolitik. Penelitian dilakukan untuk mempelajari pola aktivitas enzim ligninolitik jamur tiram pada limbah sludge pabrik kertas selama fase vegetatif sampai setelah fase generatif. Enam perlakuan yang diuji berupa tiga kombinasi komposisi medium (serbuk gergaji, sludge, campuran sludge dan serbuk gergaji), dengan dan tanpa pengayaan, yaitu penambahan dedak, kapur, dan gipsum,  serta dua strain jamur tiram Bogor (JTB) dan jamur tiram China Taipei (JTT). Pengamatan aktivitas enzim ligninolitik meliputi lakase, mangan peroksidase (Mn-P) dan lignin peroksidase  (Li-P) dilakukan sejak saat inokulasi, pada fase vegetatif (empat dan enam minggu), pada saat pembentukan primordia, fase tubuh buah, dan dua minggu setelah pembentukan tubuh buah. Masing-masing perlakuan diulang tiga kali sehingga terdapat 216 bag log jamur tiram. Hasil penelitian menunjukkan bahwa aktivitas ligninolitik dijumpai pada medium sludge dan campuran sludge+serbuk gergaji yang diino-kulasi P. ostreatus. Aktivitas enzim ligninolitik tertinggi khususnya lakase dan MnP teramati pada fase pertumbuhan vegetatif pertama yaitu sebelum terbentuknya primordia (1,697 & 2,113 U/mL, 4,394 & 2,314 U/mL  masing-masing untuk lakase JTB dan JTT dan MnP  JTB & JTT). Aktivitas LiP tertinggi dipengaruhi oleh jenis medium dan strain inokulum. Pada medium sludge, aktivitas LiP tertinggi dijumpai pada fase vegetatif (2,706 & 4,014 U/ml masing-masing untuk JTB dan JTT) sedangkan pada medium campuran sludge+serbuk gergaji, aktivitas enzim  ter-tinggi dijumpai  pada fase primordia (2,509 & 1,9 U/ml berturut-turut untuk JTB dan JTT). Pengayaan sludge meningkatkan aktivitas ligninolitik, sedangkan aktivitas lakase pada sludge diduga dapat lebih ditingkatkan dengan menambahkan serbuk gergaji disertai pengayaan berupa gipsum, dedak, dan kapur.

scholarly journals Efek Penambahan Sekam Padi Pada Berbagai Media Tanam Terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleurotus ostreotus)

Jurnal Agrium ◽  
2017 ◽  
Vol 14 (2) ◽  
pp. 18
Author(s):  
A G Rosnina ◽  
Zurrahmi Wirda ◽  
Agus Aminullah
Keyword(s):  
Rice Straw ◽  
Rice Husk ◽  
Pleurotus Ostreatus ◽  
The Body ◽  
Growth And Yield ◽  
Oyster Mushroom ◽  
Rice Husks ◽  
Growing Media ◽  
Body Diameter ◽  
Banana Leaf

The purpose of this research is observe the effect of rice husk addition on various growing media on its growth and yield of white oyster mushroom (Pleurotus ostreatus). This research used Complete Random Design (CRD) Factorial consisting of 2 factors with 3 replications. The first factor is the various media planting (M). Media of sawdust sengon (M1); Rice straw; M2 and dried banana leaf (M3). The second factor is the supply of rice husks consisting of. Without the provision of rice husk (P0); Provision of 10% rice husk (P1); Provision of rice husk 20% (P2). The best planting medium was found in the treatment of sawdust wood media sengon (M1). Besides, the addition of rice husk concentration has significant effect on all observation parameters. The best result was found in 20% rice husk. The interaction between treatment of planting media and rice husk giving significant effect on first harvest, the body diameter of fruit of first harvest and body diameter of fruit of second harvest

scholarly journals Performance of oyster mushroom (Pleurotus ostreatus) grown on different fingermillet husk substrates

2021 ◽  
Vol 4 (1) ◽  
pp. 291-300
Author(s):  
Priyanka Sanjel ◽  
Ram Kumar Shrestha ◽  
Jiban Shrestha
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Pleurotus Ostreatus ◽  
Finger Millet ◽  
Growth And Yield ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Biological Efficiency ◽  
Oilseed Cake ◽  
Mushroom Yield

Substrate type is one of the major factors affecting the growth and yield of oyster mushroom (Pleurotus ostreatus). Five substrates; Finger millet husk, Fingermillet husk + Molasses, Fingermillet husk + Rice bran, Fingermillet husk + Wheat bran, Fingermillet husk +Mustard oilseed cake, were evaluated for growth and productivity of oyster mushroom. The experiment was conducted in a completely randomized design with each treatment replicated seven times. Additives were added on finger millet husk @5% of its dry weight. Standard cultivation practice was followed with steam sterilization and spawning was done in poly bags containing 2.5 kg of wet substrate. Data were collected on time taken for full spawn run, fresh mushroom yield, biological efficiency and cropping duration upto three flushes. Molasses took the least duration (16.86 days) for full spawn run which was found to be statistically at par with wheat bran(17.13 days) and rice bran(17.75 days) whereas control treatment took the longest duration (21.62 days). Rice bran produced the highest fresh mushroom yield (793.04g/bag) with highest biological efficiency (137.92%), which was statistically at par with control, molasses and wheat bran. Similarly, rice bran had least cropping duration (66.62 days) followed by control (67.88 days), molasses (69.14 days), wheat bran (70.12 days) and mustard oilseed cake (73.86 days). The lowest fresh yield (521.84 g/bag) with lowest biological efficiency (90.75%) and the longest cropping duration (73.86 days) was observed on mustard oilseed cake supplementation. This study revealed that molasses, rice bran and wheat bran accelerated spawn run whereas mustard oilseed cake supplementation produced lowest fresh mushroom yield with least biological efficiency and highest cropping duration showing that it was ineffective for increasing yield and productivity of oyster mushroom.

scholarly journals Formulation of Inoculum a white oyster Mushroom (Pleurotus ostreatus) on powder

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 288-296
Author(s):  
Khadijah ◽  
Umrah ◽  
Orryani Lambui
Keyword(s):  
Production Process ◽  
Incubation Period ◽  
Rice Bran ◽  
Incubation Time ◽  
Natural Science ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Viability Test ◽  
Mushroom Production ◽  
Randomized Design

The study about the formulation of  inoculum a white oyster mushroom (Pleurotus ostreatus) on flour. was conducted at Biotechnology laboratory of  Departement , Faculty of  Mathematic and Natural Science, Tadulako University. This study were aimed to oyster mushroom on production. on and to abridge the cycle of oyster mushroom production process of white.peplication used Compeletely Randomized  Design (CRD) consist of six treatments and tree by comparing between  (sawduts,rice,bran, and comflour) P1(50% : 27% : 23% , P2 (50% : 30% : 20%), P3 (50% : 33% : 17%), P4 (50% : 36% : 14% ), P5 (50% : 39% : 11%), P6 (50% : 42% : 8%). There are four parameters used in this study (1) .The growth of  white oyster mushroom inoculum (2) the  incubation  time. and, (3) coloni forming unit (CFU), (4) Viability test .The resulth  showed that the best growth mycelium was 1,3cm/days with incubation period was 26 days, the highest total of CFU in P5 was 12,7x 108 CFU/ml  and was in  P3 was 3,3 x 108 CFU/ml .The inoculum viability test of production media in P5, was faster in incubation period is 18 days.

scholarly journals Pine fruit as a media for planting white oyster mushroom (Pleurotus ostreatus)

2021 ◽  
Vol 891 (1) ◽  
pp. 012025
Author(s):  
I Taskirawati ◽  
A Askar ◽  
Baharuddin
Keyword(s):  
Pleurotus Ostreatus ◽  
Cold Water ◽  
Water Immersion ◽  
Hot Water ◽  
High Rate ◽  
Oyster Mushroom ◽  
Growth Media ◽  
Alternative Materials ◽  
Research Procedure ◽  
Oyster Mushrooms

Abstract Oyster mushroom is a wood fungus that can grow naturally on dead or weathered trees (saprophytes). The commonly used planting medium for oyster mushroom cultivation is sawn waste from various trees. However, considering the high rate of deforestation, ranging from illegal logging, forest burning, and conversion of forest land functions, it is necessary to make substitutions in terms of the leading media for the growth of oyster mushrooms. In this study, pine fruit is the primary medium for the growth of oyster mushrooms to add alternative materials for growing oyster mushrooms. This study aims to determine the potential of pine fruit as a growth medium for oyster mushroom (Pleurotus ostreatus) by the soaking method using two treatments, namely cold and hot water immersion. In cold water samples, the pine cone powder was soaked for five days, seven days, and nine days for hot water soaked for 1 hour, 2 hours, and 3 hours. The research procedure started preparing oyster mushroom growth media, inoculation, maintenance and growth, and harvesting. The results showed that oyster mushrooms could grow at media soaking in hot water for 2 hours (P2) and 3 hours (P3). Treatments P2 and P3 took 78 days and 105 days, respectively, to grow oyster mushrooms.

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