scholarly journals Indole acetic acid (IAA) hormone production by endophytic bacteria isolate from Cinchona plant (Cinchona ledgerina Moens.) root

2021 ◽  
Author(s):  
Fauzi Akhbar Anugrah ◽  
Rizal Fanany ◽  
Satrio Anggoro Putra ◽  
Rahmi Masita ◽  
Desi Yulia Safitri
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Hormone Production

scholarly journals Bakteri Endofit Tanaman Jeruk Nipis (Citrus aurantifolia) Penghasil Asam Indol Asetat (AIA)

2020 ◽  
Vol 13 (2) ◽  
pp. 179-191
Author(s):  
Oktira Roka Aji ◽  
Iva Dita Lestari
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Biochemical Analysis ◽  
Crop Productivity ◽  
Bacterial Identification ◽  
Test Results ◽  
Bacterial Isolates ◽  
Characteristic Analysis ◽  
Citrus Aurantifolia

AbstrakBakteri endofit hidup dalam suatu tanaman tanpa menyebabkan gangguan bagi tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman, yaitu dengan memproduksi fitohormon seperti asam absisat, asam indol asetat, dan sitokinin. Penelitian ini bertujuan untuk mengisolasi, menyeleksi, dan mengidentifikasi bakteri endofit yang terdapat pada daun, batang, dan akar tanaman jeruk nipis (Citrus aurantifolia). Isolat bakteri endofit diseleksi berdasarkan kemampuannya dalam menghasilkan asam indol asetat (AIA). Isolat bakteri endofit ditumbuhkan pada media nutrient broth (NB) yang ditambah dengan L-triptofan. Konsentrasi AIA dihitung dengan penambahan reagen salkowski dan diukur menggunakan spektrofotometer pada panjang gelombang 530 nm. Identifikasi bakteri endofit dilakukan dengan analisis uji biokimia. Isolat bakteri endofit yang berhasil diisolasi sebanyak 12 isolat, yaitu 4 isolat dari daun, 4 isolat dari batang, dan 4 isolat dari akar. Hasil pengamatan pada uji AIA menunjukkan bahwa semua isolat bakteri endofit dapat menghasilkan hormon AIA. Isolat yang menghasilkan konsentrasi hormon AIA tertinggi adalah isolat B2 (6,51 ppm). Isolat bakteri yang berhasil diidentifikasi berasal dari genus Enterobacter, Bacillus, Pseudomonas, dan Staphylococcus. Bakteri endofit yang dapat menghasilkan AIA berpotensi dikembangkan sebagai biofertilizer untuk meningkatkan produktivitas tanaman. Abstract Endophytic bacteria live inside plants without causing disruption to plants and play an important role in stimulating plant growth. This study aims to isolate endophytic bacteria from lime plant (Citrus aurantifolia) and characterize its ability to produce indole acetic acid (IAA). Bacterial isolates were grown on media supplemented with L-tryptophan as IAA precursor. The bacterial supernatant was mixed with salkowski reagents and then measured using a spectrophotometer at 530 nm. Bacterial identification was carried out using biochemical characteristic analysis. A total of 12 endophytic bacterial isolates were successfully isolated from leaves, stem and roots of plants. Quantitative test results showed that all isolates can produce IAA. The highest concentration of IAA was produced by B2 (6.51 ppm). Biochemical analysis indicated that the isolates were from the genus Enterobacter, Bacillus, Pseudomonas and Staphylococcus. Endhophytic bacteria that can produce IAA have the potential to be developed as biofertilizers to increase crop productivity.

scholarly journals Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid)

2017 ◽  
Vol 19 (1) ◽  
pp. 76
Author(s):  
Ahmad Hanafi ◽  
Susiana Purwantisari ◽  
Budi Raharjo
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Oryza Sativa L ◽  
Block Design ◽  
Outcome Data ◽  
Bacterial Isolates ◽  
Treatment Combination ◽  
Measured Variable ◽  
Tryptophan Concentration

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH

Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

2020 ◽  
Vol 33 (4) ◽  
pp. 690-702
Author(s):  
Chellappan Soundar Raju ◽  
Abubakker Aslam ◽  
Devarajan Thangadurai ◽  
Jeyabalan Sangeetha ◽  
Krishnan Kathiravan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Direct Somatic Embryogenesis

scholarly journals Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solanum lycopersicum

2016 ◽  
Vol 21 ◽  
pp. 58-64 ◽  
Author(s):  
Abdul Latif Khan ◽  
Boshra Ahmed Halo ◽  
Ali Elyassi ◽  
Sajid Ali ◽  
Khadija Al-Hosni ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Solanum Lycopersicum ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Acc Deaminase

scholarly journals Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan

2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Agustina Monalisa Tangapo
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Sweet Potato ◽  
Growth Regulators ◽  
Endophytic Bacteria ◽  
Ipomoea Batatas ◽  
Indole Acetic Acid ◽  
Iaa Production ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan(Potential of endophytic bacteria of sweet potato (Ipomoea batatas L.) in producing Indole Acetic Acid (IAA) with the addition of L-tryptophan) Agustina Monalisa TangapoProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi:[email protected] (Article History: Received 5-01-2019; Revised 15-01-2020; Accepted 05-02-2020) ABSTRAKAsosiasi bakteri-tanaman, dapat mempengaruhi produktivitas tanaman secara langsung dan tidak langsung. Secara langsung, salah satunya yaitu bakteri dapat memproduksi dan menyekresikan zat pengatur tumbuh indole-3-acetic acid (IAA, auksin). Penelitian ini bertujuan untuk menguji kemampuan bakteri endofit ubi jalar dalam menghasilkan IAA. Metode analisis IAA dilakukan dengan metode kolorimetri. Analisis produksi IAA dilakukan dengan penambahan dan tanpa penambahan L-triptofan. Hasil penelitian menunjukkan bahwa tanpa penambahan L-triptofan, diperoleh sejumlah 19 jenis yang menghasilkan IAA dengan kisaran konsentrasi 0,29-7,21 mg/L. Dengan penambahan L-triptofan, jumlah jenis positif dan konsentrasi IAA yang dihasilkan meningkat signifikan. Jumlah jenis positif 20 jenis (91%) dan konsentrasi IAA yang dihasilkan mencapai kisaran 0,96-115,63 mg/L.Kata kunci: bakteri endofit; IAA; ubi jalar; L-triptofan ABSTRACTPlant-bacteria associations, can promote plant growth by both direct and indirect mechanisms. One of direct mechanisms is that bacteria can produce and secrete indole-3-acetic acid (IAA, auxin) growth regulators. This study aims to examine the ability of sweet potato endophytic bacteria to produce IAA. The detection of IAA production was conducted by colorimetric technique. IAA production analysis was carried out with addition and without addition of L-tryptophan. Without the addition of L-tryptophan, a total of 19 species produced IAA with a concentration range of 0.29-7.21 mg/L. With the addition of L-tryptophan, the number of positive species and the concentration of IAA produced increased significantly. The number of positive species was 20 species (91%) and the concentration of IAA produced reached a range of 0.96-115.63 mg/L.Keywords: endophytes bacterial; IAA; sweet potato; L-tryptophan

scholarly journals The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea

2017 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
LINA HERLINA ◽  
KRISPINUS KEDATI PUKAN ◽  
DEWI MUSTIKANINGTYAS
Keyword(s):  
Acetic Acid ◽  
Arachis Hypogaea ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Lateral Roots ◽  
Morphological Characteristics ◽  
Bioactive Metabolites ◽  
Biological Compounds ◽  
Three Stages

Herlina L, Pukan KK, Mustikaningtyas D. 2017. The endophytic bacteria producing IAA (Indole Acetic Acid) in Arachis hypogaea. Cell Bio Dev 1: 31-35. Endophytic bacteria are bacteria living in plant tissue and forming colony without harms the host. Every cormophyte, plants that have a stem and root, may contain some endophytic bacteria which can produce biological compounds or secondary metabolites. The objective of the study was to obtain endophytic bacteria isolates from peanut plants (Arachis hypogaea) at three locations, and to test in vitro the ability of endophytic bacteria isolates for producing IAA, and also to analyze IAA on the growth and development of mungbean plants. The study was carried out in three stages; the first was the isolation of endophytic bacteria from the leaves, stems, and roots; the second was the test of in vitro endophytic bacteria isolates to know the ability of IAA production. IAA assay was measured by using a spectrophotometer with a wavelength of 535 nm, and the third was the introduction of IAA-producing endophytic bacteria in mungbean. The parameters observed were the length of sprouts and the number of lateral roots. The results showed that 16 isolates were selected based on IAA-producing ability. The isolates could produce different IAA with different morphological characteristics. After the fourth day of incubation, the highest and the lowest of IAA amount were 69.68 (mg L-1) and 8.50 (mg L-1) respectively. Isolates that produce high IAA levels are applied to mungbeans, it affects the number of lateral roots but it does not have effect on the length of the sprouts. DM and K1K1 isolates have the effect of increasing lateral root formation and are expected to be potential sources of bioactive metabolites.

IDENTIFICATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH N2FIXATION AND INDOLE ACETIC ACID SYNTHESIS AS GROWTH PROMOTERS INCURCUMA ALISMATIFOLIAGAGNEP.

2013 ◽  
Vol 36 (9) ◽  
pp. 1424-1438 ◽  
Author(s):  
Apiraya Thepsukhon ◽  
Somporn Choonluchanon ◽  
Shikeyuki Tajima ◽  
Mika Nomura ◽  
Soraya Ruamrungsri
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Acid Synthesis ◽  
Growth Promoters
Sign in / Sign up

Export Citation Format

Share Document