scholarly journals Direct imaging of fluorescence enhancement in the gap between two gold nanodisks

2021 ◽  
Vol 118 (16) ◽  
pp. 161105
Author(s):  
Hung-Ju Lin ◽  
Hengyang Xiang ◽  
Chenghao Xin ◽  
Zhelu Hu ◽  
Laurent Billot ◽  
...  
Keyword(s):  
Fluorescence Enhancement ◽  
Direct Imaging

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

The Difference in Sensing Properties Towards Anions between Two Pyridinium Amide-Based Receptors with a Subtle Change of H-Bond Position

2020 ◽  
Vol 17 (6) ◽  
pp. 472-478
Author(s):  
Wei-tao Gong ◽  
Wei-dong Qu ◽  
Guiling Ning
Keyword(s):  
Fluorescence Enhancement ◽  
Small Difference ◽  
Fluorescence Emission ◽  
Subtle Change ◽  
Sensing Properties ◽  
Naked Eye ◽  
Recognition Ability ◽  
The Difference ◽  
L1 And L2

Two pyridinium amide-based receptors L1 and L2 with a small difference of H-bond position of the amide have been synthesized and characterized. Interestingly, they exhibited a huge difference in sensing towards AcO- and H2PO4 -, respectively. Receptor L1 was found to be ‘naked-eye’ selective for AcO- anions, while receptor L2 showed clear fluorescence enhancement selective to H2PO4 - anion. The recognition ability has been established by fluorescence emission, UV-vis spectra, and 1HNMR titration.

scholarly journals Introduction

1995 ◽  
Vol 167 ◽  
pp. 3-7
Author(s):  
A. G. Davis Philip
Keyword(s):  
Large Field ◽  
Direct Imaging ◽  
Ir Detector ◽  
New Developments ◽  
Detector Arrays ◽  
Array Detectors ◽  
Poster Sessions ◽  
Array Technology ◽  
The Subject ◽  
Scientific Results

A short introduction to the subject of the meeting, IAU Symposium No. 167, New Developments in Array Technology and Applications is given. CCD and Array detectors have become the detectors of choice at optical observatories all over the world. Direct imaging, photometry and spectroscopy are all vastly improved as a result. Thirteen IAU Commissions joined in sponsoring this meeting which indicates the wide interest in this subject. In the five days of the symposium the following topics were discussed: New Developments in CCD Technology, New Developments in IR Detector Arrays, Direct Imaging with CCDs and Other Arrays, Spectroscopy with CCDs and Other Arrays and Large Field Imaging with Array Mosaics. A few papers concerning Astrometry with CCDs were given in the poster sessions. Scientific results were also presented in the poster sessions.

Semiautomated microfluorometric instrument and reagent system for monitoring disease-related immunosuppression.

1982 ◽  
Vol 28 (9) ◽  
pp. 1887-1893 ◽  
Author(s):  
D F Ranney ◽  
A J Quattrone
Keyword(s):  
Immune Response ◽  
Dna Content ◽  
Peripheral Blood ◽  
Fluorescence Enhancement ◽  
Photon Counting ◽  
Peripheral Blood Lymphocytes ◽  
Response Modulation ◽  
Blood Lymphocytes ◽  
Complex Test ◽  
Immune Response Modulation

Abstract Several common metabolites and drugs in the serum in of patients with inflammatory, infectious, autoimmune, immunodeficient, neoplastic, and toxicant-induced diseases can produce artifactual suppression of the [methyl-3H]-thymidine assay, which is widely used to evaluate lymphocyte responsiveness. We have developed a sensitive, semiautomated, fluorescence-enhancement assay in which true immunosuppressors are measured in the presence of absence of such interfering substances. Peripheral blood lymphocytes are activated with mitogens in standard microtiter culture trays. Changes in lymphocyte DNA content are quantified with a reagent formulation containing mithramycin, the fluorescence of which is enhanced on binding to DNA in the presence of MgCl2. We solubilize cells within the intact microtiter tray by using an automated, inverted "Array Sonicator," and measure fluorescence with an automated, photon-counting fluorometer. With this system, immune response modulation can be accurately assessed in the presence of patients' sera and other complex test substances (e.g., supernates from hybridomas, fermentation vats, viral preparations, and macrophage cultures.

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