Annona muricata leaves ethanolic extract in water, n-hexane, and methanol fractions: Cytotoxicity assay, antioxidant activity, flavonoid content, polyphenol content, and acetogenin content

2021 ◽  
Author(s):  
Elsa Anisa Krisanti ◽  
Afidya Indina Harumanti ◽  
Kamarza Mulia
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Cytotoxicity Assay ◽  
Annona Muricata ◽  
Flavonoid Content ◽  
Polyphenol Content

scholarly journals Antioxidant Activity, Total Polyphenol, Flavonoid and Tannin Contents of Fermented Green Coffee Beans with Selected Yeasts

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 29 ◽  
Author(s):  
Mesfin Haile ◽  
Won Kang
Keyword(s):  
Antioxidant Activity ◽  
Green Coffee ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Fermentation Time ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
The Mean

We examined the antioxidant activity, total polyphenol content (TPC), total flavonoid content (TFC), total tannin content (TTC) and physical characteristics of green coffee beans fermented with selected yeasts. There was no significant (p > 0.05) interaction effect between yeast-fermented coffee extracts and duration of fermentation on antioxidant activity (oxygen radical absorbance capacity [ORAC] and superoxide dismutase-like [SOD-like] activity). However, the mean of the antioxidant activity (ORAC and SOD-like activity) significantly (p < 0.05) increased in the fermented coffee extracts compared to unfermented coffee. There were significant (p < 0.05) interaction effects between yeast-fermented coffee extracts and duration of fermentation (24 h and 48 h) on the TPC, TFC, TTC and pH of the fermented solution and on the colors of the ground-roasted coffee. The TPC showed a pattern of increase in samples Ferm-1 and Ferm-3 as fermentation time increased from 24 h to 48 h. However, a decreasing TPC trend was observed in Ferm-2 as the number of fermentation hours increased from 24 to 48. The fermented coffee beans had a significantly higher flavonoid content than the unfermented coffee beans, while fermentation significantly decreased the tannin content compared to that in unfermented coffee.

scholarly journals Evaluation of Phytochemical and Antioxidant Activity of Gomphrena celosioides Mart. Grown in Tien Giang Province, Vietnam

2019 ◽  
Vol 32 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Nguyen Van Thuy ◽  
Nguyen Minh Tien ◽  
Nguyen Ngoc Quy ◽  
Tri Duc Lam ◽  
Huynh Thi Kieu Linh ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ether Extract ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Polyphenol Compounds ◽  
Diethyl Ether Extract ◽  
Gomphrena Celosioides ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds

Gomphrena celosioides Mart. is well known for its medicinal values worldwide. In this study, three extracts, viz. diethyl ether extract (DEE), ethanolic extract (EE) and aqueous extract (AE), were successively obtained from the leaves and stem of Gomphrena celosioides to determine the polyphenol and flavonoid content in this plant. A wide variety of pharmacologically active compounds such as alkaloid, flavonoid, terpenoid, saponin, tannin and polyphenol compounds were present in Gomphrena celosioides. The results of quantitative determination showed that total polyphenol content of DEE, EE and AE reached 35.35 ± 1.47, 250.17 ± 2.95 and 133.92 ± 3.17 mgGAE/g, respectively. Moreover, total flavonoid content of the DEE, EE and AE was 23.21 ± 1.87, 50.74 ± 2.32 and 27.25 ± 1.34 mgQE/g, respectively. In comparison with DEE and AE, the ethanolic extract exhibited the highest DPPH (IC50 = 13.29 ± 0.10 μg/mL) and ABTS (IC50 = 6.3 ± 0.11 μg/mL).

scholarly journals Evaluation of phenolic, flavonoid contents and antioxidant activity in leaves, stem and roots of Solanum indicum Linn.

2020 ◽  
Vol 21 (1&2) ◽  
pp. 167-172
Author(s):  
Brij Mohan ◽  
Arun Kakkar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Flavonoid Content ◽  
Successive Extraction ◽  
Soxhlet Apparatus ◽  
Solanum Indicum

The present investigation deals with the evaluation of phenolic, flavonoid contents and antioxidant activity in leaves, stem and roots of Solanum indicum. Powdered samples of leaves, stem and roots were subjected to successive extraction with solvents of increasing polarities i.e. Ethanol, Water: Ethanol (Aqua- alcoholic) (20: 80) and Water (aqueous) using soxhlet apparatus. The results showed the maximum phenolic content (12.351±0.03%) in Aqua- alcoholic (80: 20) extract and maximum flavonoid content (38.658±0.05%) in ethanolic extract of leaves. For stem, both the maximum phenolic (11.750±0.02%) and flavonoid (2.040±0.03%) contents was found in Aqua- alcoholic (80: 20) extract. Similarly, the maximum phenolic (18.651±0.03%) and flavonoid (2.701±0.05%) contents was found in ethanolic extract of roots. The Aqua- alcoholic (80: 20) extracts for leaves and stem and ethanolic extract for roots was found to contain the maximum antioxidant activity.

scholarly journals Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Najma Annuria Fithri ◽  
Fitrya Fitrya ◽  
Tia Shabrina ◽  
Diva Yulanri
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Structural Stabilization ◽  
Dpph Method ◽  
Research Outcome ◽  
Phenolic And Flavonoid

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

scholarly journals FOURIER-TRANSFORM INFRARED SPECTROSCOPY STUDIES AND EVALUATION OF TOTAL PHENOLICS, FLAVONOIDS, AND ANTIOXIDANT ACTIVITY OF CLEOME GYNANDRA

2019 ◽  
pp. 214-218 ◽  
Author(s):  
RAMYA KUBER BANOTH ◽  
ASHWINI THATIKONDA
Keyword(s):  
Antioxidant Activity ◽  
Colorimetric Assay ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Cleome Gynandra ◽  
Dpph Radical Scavenging

Objective: The objective of this study was to evaluate the nature of chemical constituents, total phenolics, total flavonoids, and antioxidant activity of Cleome gynandra and their functional groups with the help of phytochemical, Fourier-transform infrared spectroscopy (FTIR) analysis, colorimetric assay, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methods: C. gynandra of the Cleomaceae family is an annual herb. The dried leaves were powdered and extracted using Soxhlet apparatus by different solvents. Preliminary phytochemical analysis was carried out to identify the phytoconstituents present in the extract of C. gynandra, FTIR spectrum was scanned at the range of 4000-400 cm−1. The extracts were subjected to the colorimetric assay in triplicate manner to quantitative determination of total phenolic and total flavonoid content. Gallic acid and rutin used as standards to determine the total phenolic content and total flavonoid content. Antioxidant activity was evaluated using DPPH radical scavenging method. Results: Phytochemical analysis of the ethanolic extract of C. gynandra revealed the presence of alkaloids, phenolics, saponins, steroids, flavonoids, cardiac glycosides, and tannins. FTIR spectrum showed intense bands at 3679.18, 3616.63, 3317.34, 2943.67, 1634.01, 1360.20, 1036.71, and 778.04 cm−1 corresponding to N-H2, O-H stretch, aliphatic C-H stretch, C=O, C-H benzene, C-O stretch, and C-Cl. The total phenolic content was found to be 8.39 ± 0.0952 mg gallic acid equivalent/g and 66.76 ± 0.0333 mg rutin equivalent/g. The DPPH radical scavenging activity of ethanolic extract was showed more scavenging activity compared to ethyl acetate and n-hexane fractions. Conclusion: The present research work creates a platform to screen many bioactive chemical constituents present in C. gynandra to treat various diseases.

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