Peculiarities of the methylene blue monomeric form redox process in the field of low-frequency exposure

2020 ◽  
Author(s):  
N. A. Bogatov ◽  
A. S. Savina
Keyword(s):  
Methylene Blue ◽  
Low Frequency ◽  
Redox Process ◽  
Monomeric Form

scholarly journals Inhibition of oxidated reducing reactions in the field of low frequency impacts

2019 ◽  
Vol 487 (3) ◽  
pp. 275-278
Author(s):  
G. N. Fadeev ◽  
V. S. Boldyrev ◽  
N. A. Bogatov ◽  
A. L. Nikolaev
Keyword(s):  
Methylene Blue ◽  
Ascorbic Acid ◽  
Acoustic Field ◽  
Chemical Process ◽  
Low Frequency ◽  
Redox Process ◽  
Redox Processes ◽  
Active Components ◽  
Frequency Effects ◽  
Low Frequency Oscillations

The effect of low-frequency oscillations on a system consisting of two biochemically active components: the dye of methylene blue and ascorbic acid is investigated. Each of these components can be reversibly oxidized and reduced. The system is interesting in that it makes it possible to trace the influence of the low-frequency acoustic field on the mutual redox process and to reveal the features of the effect of such oscillations. It was found that, in this system, with the introduction of low-frequency effects, redox processes are not accelerated, but slowed down. There is an effect inhibition of the sound-chemical process in the field of low-frequency acoustic effects. The effect is quantified.

scholarly journals Acid-Basic Activation of Carbon-Containing Sorption Material and Its Application for Extraction of Methylene Blue Dye from Aquatic Media

2021 ◽  
pp. 029-038
Author(s):  
I. V. Starostina ◽  
D. O. Polovneva ◽  
Yu. L. Makridina ◽  
E. V. Loktionova
Keyword(s):  
Methylene Blue ◽  
Acid Treatment ◽  
Sodium Hydroxide Solution ◽  
Low Frequency ◽  
Sorption Isotherms ◽  
Frequency Region ◽  
Carboxyl Groups ◽  
Blue Dye ◽  
Total Acidity ◽  
Sorption Material

The results of the use of thermally modified kieselguhr sludge from the production of vegetable oils (TKSH500) of the initial and activated with a 30 % solution of nitric acid (TKSH500k) and 1M sodium hydroxide solution (TKSH500sh) for purification of model solutions from the dye methylene blue (MB) are presented. The maximum activating effect was achieved as a result of acid treatment, which leads to a 38 % increase in the total acidity of the sorption material in comparison with the initial one. It is shown that the sorption isotherms of the dye on the initial and activated samples of TKSH500 have an S-shaped form. In the region of low dye concentrations, a monolayer is formed; with an increase in the dye content, sorption becomes polymolecular. Analysis of the IR spectra of sorption materials after adsorption of the MB dye showed a shift to the low-frequency region of the positions of the bands in the regions corresponding to the vibrations of the O–H and C = O bonds, which indicates the ionization of hydroxyl and carboxyl groups present in the sorption materials, and the formation of a bond between them and the MB molecule.

Paper spray mass spectrometry applied in the monitoring of a chemical system in dynamic chemical equilibrium: the redox process of methylene blue

2016 ◽  
Vol 30 (9) ◽  
pp. 1176-1180 ◽  
Author(s):  
Camila Cristina Almeida de Paula ◽  
Alberto Valadares ◽  
Marina Jurisch ◽  
Evandro Piccin ◽  
Rodinei Augusti
Keyword(s):  
Mass Spectrometry ◽  
Methylene Blue ◽  
Chemical Equilibrium ◽  
Redox Process ◽  
Chemical System ◽  
Paper Spray

scholarly journals Significant Alteration in the Stoichiometry of Factor VIII Inhibitor Interactions in the Presence of 0.25 M CaCl2

1977 ◽  
Author(s):  
C. B. Harbury ◽  
P. L. Perry ◽  
M. E. Janszen
Keyword(s):  
Factor Viii ◽  
Low Frequency ◽  
Factor Viii Inhibitor ◽  
Significant Activity ◽  
Monomeric Form ◽  
Antibody Affinity ◽  
Donor Pool ◽  
Incubation Experiments ◽  
Viii Inhibitor ◽  
Antigen Antibody

The major aim of this study was to assess whether the hemophilic VIII antibody (Ab) interacted more readily with “monomeric” factor VIII than with undissociated VIII. Conditions were chosen so that multiplicity of neutralization would occur with low frequency, favoring one Ab molecule per VIII molecule. Remaining VIII (R8) was kept at 65% to 85% of original VIII activity. Ab units were calculated by units of VIII neutralized x 1/dilution of antisera. As R8 fell below 65%, the apparent potency of Ab units decreased. A 25-donor pool of lyophilized plasma was made up with H2O or 0.25 M CaCl2. Thus, within and between experiments, unknown but constant concentrations of VIII molecules and VIII antigens were present. Unknown but constant concentrations and types of Ab molecules from each of three inhibitor plasmas were used. Samples were incubated 2 hours @ 22° C. Controls remained stable for the duration. The two-stage factor VIII assay of Pool et al allowed sufficient dilution of CaCl2 for accurate assay.For 15 independent experiments, = 53.4 ± 12 Ab u/ml and = 25.8 ± 10 Ab u/mlFor 31 independent experiments, = 437 ± 78 Ab u/ml and = 174 ± 50 Ab u/mlFor 12 independent experiments, = 80 ± 9 Ab u/ml and = 32 ± 9 Ab u/mlThus, there was 2.07, 2.51, and 2.50 times more VIII neutralized in the 0.25 M CaCl2 vs. H2O incubation. Experiments were also run at 37° C, slightly increasing Ab u/ml for H2O; but the H2O control often lost significant activity. Possible explanations include: 1) the antibody is bivalent when VIII is monomeric; 2) Previously-hidden Vlll-neutralizing Antigen sites are revealed in the VIII monomeric form; or 3) antigen/antibody affinity is altered.

A Study on the Fine Structure of the Lateral Line Organ of the Sea Eel

1973 ◽  
Vol 31 ◽  
pp. 648-649
Author(s):  
K. Hama
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Lateral Line ◽  
Low Frequency ◽  
Sensory Cells ◽  
Apical Surface ◽  
Voltage Electron ◽  
Sensory Epithelia ◽  
Low Frequency Vibration ◽  
Transmission Electron

The lateral line organs of the sea eel consist of canal and pit organs which are different in function. The former is a low frequency vibration detector whereas the latter functions as an ion receptor as well as a mechano receptor.The fine structure of the sensory epithelia of both organs were studied by means of ordinary transmission electron microscope, high voltage electron microscope and of surface scanning electron microscope.The sensory cells of the canal organ are polarized in front-caudal direction and those of the pit organ are polarized in dorso-ventral direction. The sensory epithelia of both organs have thinner surface coats compared to the surrounding ordinary epithelial cells, which have very thick fuzzy coatings on the apical surface.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Lectin Binding to Human Breast Tumor Cells

1976 ◽  
Vol 34 ◽  
pp. 34-35
Author(s):  
Robert E. Nordquist ◽  
J. Hill Anglin ◽  
Michael P. Lerner
Keyword(s):  
Carcinoma Cell ◽  
Ricinus Communis ◽  
Lectin Binding ◽  
Low Frequency ◽  
Breast Carcinoma Cell Line ◽  
Human Breast ◽  
Human Breast Tumor ◽  
Duct Carcinoma ◽  
Specific Antigens ◽  
Ricin Agglutinin

A human breast carcinoma cell line (BOT-2) was derived from an infiltrating duct carcinoma (1). These cells were shown to have antigens that selectively bound antibodies from breast cancer patient sera (2). Furthermore, these tumor specific antigens could be removed from the living cells by low frequency sonication and have been partially characterized (3). These proteins have been shown to be around 100,000 MW and contain approximately 6% hexose and hexosamines. However, only the hexosamines appear to be available for lectin binding. This study was designed to use Concanavalin A (Con A) and Ricinus Communis (Ricin) agglutinin for the topagraphical localization of D-mannopyranosyl or glucopyranosyl and D-galactopyranosyl or DN- acetyl glactopyranosyl configurations on BOT-2 cell surfaces.

Practical High Resolution Microscopy

1968 ◽  
Vol 26 ◽  
pp. 302-303
Author(s):  
P. A. Marsh ◽  
T. Mullens ◽  
D. Price
Keyword(s):  
High Resolution ◽  
Magnetic Fields ◽  
Low Frequency ◽  
Resolving Power ◽  
Careful Attention ◽  
Electron Microscopes ◽  
The Relationship ◽  
High Resolution Microscopy ◽  
New Facilities

It is possible to exceed the guaranteed resolution on most electron microscopes by careful attention to microscope parameters essential for high resolution work. While our experience is related to a Philips EM-200, we hope that some of these comments will apply to all electron microscopes.The first considerations are vibration and magnetic fields. These are usually measured at the pre-installation survey and must be within specifications. It has been our experience, however, that these factors can be greatly influenced by the new facilities and therefore must be rechecked after the installation is completed. The relationship between the resolving power of an EM-200 and the maximum tolerable low frequency interference fields in milli-Oerstedt is 10 Å - 1.9, 8 Å - 1.4, 6 Å - 0.8.

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