scholarly journals Morphological and biochemical characteristic of endosymbiont cellulolytic bacteria from gut of Hypothenemus hampei Ferr. and its enzyme activity

2020 ◽  
Author(s):  
Azizah ◽  
Purwatiningsih ◽  
Hidayat Teguh Wiyono ◽  
Kahar Muzakhar
Keyword(s):  
Enzyme Activity ◽  
Biochemical Characteristic ◽  
Hypothenemus Hampei ◽  
Cellulolytic Bacteria

Enzyme Activity of Cellulolytic Bacteria from Biological Education and Research Forest Floor Andalas University

2021 ◽  
Vol 24 (11) ◽  
pp. 1138-1143
Author(s):  
Fuji Astuti Feb ◽  
Chairul . ◽  
Anthoni Agustien ◽  
Riri Kurnia Ila ◽  
Elsa Rohmah
Keyword(s):  
Enzyme Activity ◽  
Forest Floor ◽  
Cellulolytic Bacteria ◽  
Biological Education

scholarly journals Produksi Enzim Selulase Kasar dari Isolat Bakteri B2S8 menggunakan Substrat Brangkasan Jagung dengan Perlakuan Konsentrasi Inokulum dan Komposisi Media yang berbeda

2020 ◽  
Vol 8 (2) ◽  
pp. 267
Author(s):  
Nursatria Purba ◽  
Ida Bagus Wayan Gunam ◽  
I Made Mahaputra Wijaya
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Corn Stover ◽  
Bacterial Isolate ◽  
Specific Activity ◽  
Block Design ◽  
Cellulolytic Bacteria ◽  
Endoglucanase Activity ◽  
Cellulase Enzyme ◽  
The Media

The purpose of this study was determined the media and concentration of cellulolytic bacterial isolates to produce high cellulase enzyme activity. Production of crude cellulase enzyme in media and concentration of different bacterial isolate used a factorial Randomized Block Design (RBD) which consist of two factors. The first factor was the media production of different cellulase enzyme consisting of 3 levels, namely media 1, 2 and 3. The second factor was the concentration of bacterial isolate consisting of 5 levels namely 1, 2, 3, 4 and 5%. This study used a B2S8 cellulolytic bacterial isolate that has the highest value of cellulase enzyme activity and the highest degradation rate of cellulose in previous studied and determined the ability of exoglucanase enzyme activity, endoglucanase enzyme and dissolved protein content produced from cellulolytic bacterial isolate. This study used Carboxymethyl Cellulose (CMC) for enzyme activity test and 1% corn stover as a substrate on the media to produce crude cellulase enzyme. The result showed that the highest cellulase enzyme activity in the third media and 5% cellulolytic bacterial inoculum concentration resulted in endoglucanase activity of 0.0332 IU/mL, exoglucanases enzyme activity of 0.0060 IU/mL, dissolved protein content in the amount of 0.5670 mg/mL, the specific endoglucanase activity of 0.0807 IU/mg and the specific activity of exoglucanase of 0.0123 IU/mg. Keywords: Cellulolytic bacteria, Cellulase enzymes, Enzyme activity, Corn stover

scholarly journals Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

2016 ◽  
Vol 8 (3) ◽  
pp. 386
Author(s):  
Wijanarka Wijanarka ◽  
Endang Kusdiyantini ◽  
Sarjana Parman
Keyword(s):  
Enzyme Activity ◽  
Digestive Tract ◽  
Generation Time ◽  
Cellulase Activity ◽  
Biology Education ◽  
Activity Measurement ◽  
Bacterial Isolates ◽  
Achatina Fulica ◽  
Cellulolytic Bacteria ◽  
Activity Measurements

<p>On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (<em>Achatina fulica</em>). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate (µ) 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).</p><p><strong>How to Cite</strong></p><p>Wijanarka, W., Kusdiyantini, E. &amp; Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (<em>Achatina fulica</em>) and Test the Ability of Cellulase Activity. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 386-392. </p>

Performances of Dominant Thermophilic Cellulolytic Bacteria for the Bio-Drying

2013 ◽  
Vol 777 ◽  
pp. 178-181
Author(s):  
Yu Lan Tang ◽  
Jia Long Zhang ◽  
Li Yan Wang ◽  
Jin Yu ◽  
Jun Liu ◽  
...  
Keyword(s):  
Weight Loss ◽  
Enzyme Activity ◽  
Water Content ◽  
Filter Paper ◽  
Cellulolytic Bacterium ◽  
Cellulolytic Bacteria ◽  
Dewatered Sludge ◽  
Drying Conditions

Six dominant thermophilic cellulolytic bacteria were separated from undigested dewatered sludge. By analysis of their water content, value of hydrolysis up, rate of weight loss of filter paper and CMC-enzyme activity, a dominant thermophilic cellulolytic bacterium named XW5was separated and inoculated in the dewatered sludge under simulating bio-drying conditions. The results showed that water content of the dewatered sludge which was inoculated dominant bacterium was decreased from an initial 70.05% to 62.49%.

Effects of fibrolytic enzymes and isobutyrate on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in pre- and post-weaning dairy calves

2019 ◽  
Vol 59 (3) ◽  
pp. 471 ◽  
Author(s):  
C. Wang ◽  
Q. Liu ◽  
G. Guo ◽  
W. J. Huo ◽  
Y. X. Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Volatile Fatty Acids ◽  
Dairy Calves ◽  
Ruminal Fermentation ◽  
Cellulolytic Bacteria ◽  
Fibrolytic Enzymes ◽  
Ruminal Ph ◽  
Bull Calves ◽  
Microbial Enzyme ◽  
Microbial Enzyme Activity

The objective of the present study was to evaluate the effects of fibrolytic enzymes (FE, containing 160 units of cellulase and 4000 units of xylanase) or isobutyrate (IB) supplementation on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in dairy calves. Forty-eight Holstein bull calves of 15 days of age and of 44.9 ± 0.28 kg of BW were randomly assigned to four groups in a 2 × 2 factorial arrangement. Two levels of FE (0 g (FE–) or 1.83 g per calf per day (FE+)) and IB (0 g (IB–) or 6 g per calf per day (IB+)) were added. Calves were weaned at 60-day-old and four calves were selected from each treatment at random and slaughtered at 45 and 90 days of age. There was no IB × FE interaction effect. Ruminal pH decreased with IB or FE supplementation for post-weaned calves, whereas concentrations of total volatile fatty acids and acetate increased with IB or FE supplementation for pre- and post-weaned calves. Acetate to propionate ratio increased with IB supplementation, but was unaffected by FE supplementation. Ammonia-N concentration decreased with IB or FE supplementation for pre- and post-weaned calves. For post-weaned calves, activities of CMCase increased with IB or FE supplementation, and activities of cellobiase, xylanase, pectinase, β-amylase and protease increased with IB supplementation. Populations of B. fibrisolvens and F. succinogenes for pre- and post-weaned calves and R. flavefaciens for post-weaned calves increased with IB or FE supplementation. It is suggested that ruminal fermentation and growth performance of calves was improved with IB and FE supplementation, and the combination of IB and FE has the potential to stimulate the growth of pre- and post-weaned dairy calves.

scholarly journals Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province

DEPIK ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 243-250
Author(s):  
Irma Dewiyanti ◽  
Darmawi Darmawi ◽  
Zainal Abidin Muchlisin ◽  
Teuku Zahrial Helmi ◽  
Iko Imelda Arisa ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Methyl Cellulose ◽  
Cellulase Activity ◽  
Mangrove Ecosystem ◽  
Microbiology Laboratory ◽  
Cellulolytic Bacteria ◽  
Cellulase Enzymes ◽  
Cellulase Enzyme ◽  
Aceh Province

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian //  TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

scholarly journals Cellulolytic Bacteria Screened from Qinling (China) for Biomass Degradation and Cellulases First Cloned from Bacillus methylotrophicus

2019 ◽  
Author(s):  
Lingling Ma ◽  
Yingying Lu ◽  
Hong Yan ◽  
Xin Wang ◽  
Yanglei Yi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Carbon Source ◽  
Wheat Straw ◽  
Corn Stover ◽  
Sugar Content ◽  
Qinling Mountains ◽  
Cellulolytic Bacteria ◽  
Biomass Degradation ◽  
Bacillus Methylotrophicus

Abstract Background Cellulosic biomass degradation still needed more studies while bioenergy is becoming mainly energy in future and more evaluate bacteria isolation laid a foundation of further study. Qinling Mountains have unique biodiversity, acting as promising source of cellulose-degrading bacteria exhibiting noteworthy properties. The aim of this work was to find potential cellulolytic bacteria in depredating multiform carbon source cellulose substrate. Results In this study, 55 potential cellulolytic bacteria screened out and were identified. Based on the results of the investigation of cellulase activities and reducing sugar content via different carbon substrate effect, Bacillus methylotrophicus 1EJ7, Bacillus subtilis 1AJ3 and Bacillus subtilis 3BJ4 were further taken to hydrolyze wheat straw, corn stover and switchgrass, suggesting that B. methylotrophicus 1EJ7 was the most preponderant bacterium, obtaining highest sugar content (95mg/100mL) in switchgrass, wheat straw and corn stover. Scanning electron microscopy (SEM) and X-ray diffraction results of wheat straw surface and crystallinity indicated the hydrolyzation. By ascertaining the target sequence of cellulase for the cloning and expression in an economical and convenient manner, the genes of β-glucosidase (243 aa) and endoglucanase (499 aa) of B. methylotrophicus 1EJ7. Recombinant β-glucosidase from GH16 family and enzyme activity was 1670.15±18.94 U/mL. Endoglucanase consist of GH5 family catalytic domain and a carbohydrate-binding module belongs to CBM3 family and enzyme activity was 0.130±0.002 U/mL. Conclusions Screened, identified the cellulolytic bacteria from rotten wood of Qinling Mountains and explored their ability in degrading different carbon source cellulose substrate, including purified and natural carbon sources. Bacillus were the predominant species among the isolated strains, and Bacillus methylotrophicus 1EJ7 performant well on cellulose degradation. In the meantime, the β-glucosidase and endoglucanase were successfully cloned and expressed from Bacillus methylotrophicus for the first time. The strain and the recombinant enzyme have potential application in industrial production.

scholarly journals Cellulase enzyme activity of the bacteria isolated from mangrove ecosystem in Aceh Besar and Banda Aceh

2022 ◽  
Vol 951 (1) ◽  
pp. 012113
Author(s):  
I Dewiyanti ◽  
D Darmawi ◽  
Z A Muchlisin ◽  
T Z Helmi ◽  
I I Arisa ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Congo Red ◽  
Cellulase Activity ◽  
Mangrove Ecosystem ◽  
Microbiology Laboratory ◽  
Cellulolytic Bacteria ◽  
Clear Zone ◽  
Cellulase Enzyme ◽  
Mangrove Soil ◽  
Quarantine Station

Abstract Cellulolytic bacteria is one of the beneficial bacteria that can found in mangrove ecosystem. The purposes of study were to analyse the cellulolytic index, and to analyse the cellulase activity of bacteria isolated from soil mangrove. Qualitatively, assessment of cellulase activity were carried out at the Microbiology laboratory of Fish Quarantine Station, Quality Control and Safety of Fishery Products (SKIPM) Aceh, while quantitatively was observed in microbiology laboratory, Biology Department, IPB. Assessment of qualitative cellulase activity is performed by growing the selected pure isolate on 1% CMC medium then spilled 1% congo red to test its cellulolytic potential. Cellulolytic potential was determined by clear zone performed around the colony after congo red flooded. The quantitative cellulase enzyme activity test carried out by DNS method tested on one selective isolate. There were 21 from 39 isolates showed a clear zone isolated from mangrove soil. The cellulolytic index (CI) obtained ranged from 0.07 to 0.80 classified as low cellulolytic index criteria. The cellulolytic index was higher in bacteria isolated from mangrove rehabilitated than mangrove unrehabilitated. The highest cellulase activity and specific cellulase activity of BTMD32 was at 48 hours with the value were 0.0012 U/ml, 0.077 U/mg, respectively. The result concluded that the bacteria cellulolytic isolated from mangrove soil had low cellulolytic index, low cellulase activity, and low specific cellulase activity.

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