Engineering control circuits for molecular robots using synthetic biology

Ting-Yen Wei; Warren C. Ruder

doi:10.1063/5.0020429

Sensing and Sensibility: Programming Smarter Chimeric Antigen Receptor T Cells

Blood ◽

10.1182/blood-2019-121120 ◽

2019 ◽

Vol 134 (Supplement_1) ◽

pp. SCI-24-SCI-24

Author(s):

Wendell Lim

Keyword(s):

T Cells ◽

Tumor Microenvironment ◽

Synthetic Biology ◽

Cell Design ◽

Strong Response ◽

Modular Control ◽

Car T Cells ◽

Car T ◽

Control Circuits ◽

Recognition And Response

CAR T cells have shown remarkable outcomes when targeting specific hematologic cancers. But targeting solid cancers has proven difficult: it is difficult to identify unique antigens, to traffic to tumors, and to mount a strong response in the solid tumor microenvironment. To overcome these issues, we are using the approaches of synthetic biology to engineer next-generation T cells. We are engineering cells that utilize new sets of sensors and modular control circuits that enable them to execute new recognition and response programs with higher precision and robustness. These new capabilities may allow us to develop therapeutic T cells that are far more effective and safe against solid tumors. Disclosures Lim: Cell Design Labs: Other: founder of Cell Design Labs; acquired by Gilead in 2017; Allogene: Consultancy.

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Synthetic Approaches to Biology

Emerging Research on Bioinspired Materials Engineering - Advances in Chemical and Materials Engineering ◽

10.4018/978-1-4666-9811-6.ch012 ◽

2016 ◽

pp. 323-351

Author(s):

Robert Penchovsky ◽

Martina Traykovska

Keyword(s):

Synthetic Biology ◽

De Novo ◽

Biological Species ◽

Gene Control ◽

Whole Genomes ◽

Control Circuits ◽

Synthetic Approaches ◽

Integrate Research

Nanobiotechnology and synthetic biology are emerging as novel fields that integrate research from science and technology to create novel organisms with new desired properties. We present here the new revolutionary methods of synthetic biology that enable us to engineer gene control circuits, edit genomes, and create de novo whole genomes. The creation of new genomes that function in the cell means that we can create new organisms that are different from those observed in nature. The synthetic genomes can contain novel combinations of genes that offer the opportunities to create novel biological species that possess predefined combination of properties. Therefore, the synthetic genomes can be regarded as a new kind of materials. The methods for whole genome assemble applied so far combined several in vitro and in vivo steps that possess certain technical limitations and shortcomings. In this chapter, we discuss all technical aspects of assembling novel genomes and their current limitations. The genome editing technologies that have been developed over the last several years based on the CRISPR-Cas system is also discussed. In addition, we present major RNA-based methods for design of gene control circuits both in prokaryotes and eukaryotes, including humans.

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Investigation of irradiation-induced nucleation processes in silicon and germanium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100137495 ◽

1995 ◽

Vol 53 ◽

pp. 224-225

Author(s):

Harry A. Atwater ◽

C.M. Yang ◽

K.V. Shcheglov

Keyword(s):

Room Temperature ◽

Crystal Size ◽

Initial Distribution ◽

Engineering Control ◽

Size Evolution ◽

Silicon And Germanium ◽

Ge Quantum Dot ◽

And Control ◽

Germanium Quantum Dots ◽

Kinetics Of

Studies of the initial stages of nucleation of silicon and germanium have yielded insights that point the way to achievement of engineering control over crystal size evolution at the nanometer scale. In addition to their importance in understanding fundamental issues in nucleation, these studies are relevant to efforts to (i) control the size distributions of silicon and germanium “quantum dots𠇍, which will in turn enable control of the optical properties of these materials, (ii) and control the kinetics of crystallization of amorphous silicon and germanium films on amorphous insulating substrates so as to, e.g., produce crystalline grains of essentially arbitrary size.Ge quantum dot nanocrystals with average sizes between 2 nm and 9 nm were formed by room temperature ion implantation into SiO2, followed by precipitation during thermal anneals at temperatures between 30°C and 1200°C[1]. Surprisingly, it was found that Ge nanocrystal nucleation occurs at room temperature as shown in Fig. 1, and that subsequent microstructural evolution occurred via coarsening of the initial distribution.

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Singapore bets big on synthetic biology

Nature ◽

10.1038/d41586-018-04123-2 ◽

2018 ◽

Cited By ~ 2

Author(s):

Sandy Ong

Keyword(s):

Synthetic Biology

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GondenBraid2.0: A comprehensive toolkit for plant synthetic biology

Planta Medica ◽

10.1055/s-0033-1351820 ◽

2013 ◽

Vol 79 (13) ◽

Author(s):

A Sarrion-Perdigones ◽

M Vazquez-Vilar ◽

J Palaci ◽

A Granell ◽

D Orzáez

Keyword(s):

Synthetic Biology ◽

Plant Synthetic Biology

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86. Engineering Control to Prevent the Spread of Tuberculosis Among Health Care Workers in Thailand

10.3320/1.2762867 ◽

1999 ◽

Cited By ~ 1

Author(s):

P.A. Jensen ◽

W. Uthaivorawit ◽

D. Garrett ◽

P. Zuber ◽

K. Limpakarnjanarat

Keyword(s):

Health Care ◽

Health Care Workers ◽

Engineering Control ◽

Care Workers

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Life as a Raw Material: Illusions of Control

Somatechnics ◽

10.3366/soma.2012.0060 ◽

2012 ◽

Vol 2 (2) ◽

pp. 250-262 ◽

Cited By ~ 2

Author(s):

Oron Catts ◽

Ionat Zurr

Keyword(s):

Synthetic Biology ◽

Cognitive Engineering ◽

Raw Material ◽

Future Scenarios ◽

Short History ◽

Know How ◽

Political Forces ◽

History Of ◽

The Way

The paper discusses and critiques the concept of the single engineering paradigm. This concepts allude to a future in which the control of matter and life, and life as matter, will be achieved by applying engineering principles; through nanotechnology, synthetic biology and, as some suggest, geo-engineering, cognitive engineering and neuro-engineering. We outline some issues in the short history of the field labelled as Synthetic Biology. Furthermore; we examine the way engineers, scientists, designers and artists are positioned and articulating the use of the tools of Synthetic Biology to expose some of the philosophical, ethical and political forces and considerations of today as well as some future scenarios. We suggest that one way to enable the possibilities of alternative frames of thought is to open up the know-how and the access to these technologies to other disciplines, including artistic.

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