scholarly journals Genetic variation analysis of melon (Cucumis melo L. ‘Tacapa Gold’) using inter-simple sequence repeat

2020 ◽  
Author(s):  
Budi Setiadi Daryono ◽  
Fadilla Husnun ◽  
Dian Sartika
Keyword(s):  
Genetic Variation ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Inter Simple Sequence Repeat ◽  
Variation Analysis ◽  
Sequence Repeat ◽  
Cucumis Melo L ◽  
Simple Sequence

scholarly journals Determination of Genome Size, Chromosome Number, and Genetic Variation Using Inter-Simple Sequence Repeat Markers in Lotus spp.

CYTOLOGIA ◽  
2016 ◽  
Vol 81 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Hidenori Tanaka ◽  
Awatsaya Chotekajorn ◽  
Sayumi Kai ◽  
Genki Ishigaki ◽  
Masatsugu Hashiguchi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Chromosome Number ◽  
Genome Size ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence

Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic

2008 ◽  
Vol 88 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Xiuliang Wang ◽  
Fengjuan Zhao ◽  
Zimin Hu ◽  
Alan T. Critchley ◽  
Steve L. Morrell ◽  
...  
Keyword(s):  
Genetic Variation ◽  
North Atlantic ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Chondrus Crispus ◽  
Sequence Repeat ◽  
Issr Analysis ◽  
Simple Sequence

scholarly journals Deteksi Gen Ketahanan Terhadap Gsb-4 (Gummy Stem Blight) pada Tanaman Melon (Cucumis Melo L.)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Ganies Riza Aristya Aisha Rizky Rahmawati Budi Setiadi Daryono
Keyword(s):  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Cucumis Melo L ◽  
Simple Sequence

Perakitan melon tahan penyakit gummy stem blight (Gsb-4) merupakan upaya yang dapat dilakukan untuk menanggulangi penyakit gummy stem blight. Untuk itu harus diketahui ada tidaknya gen ketahanan terhadap gummy stem blight. Tujuan penelitian ini adalah mendeteksi adanya gen ketahanan terhadap gummy stem blight pada 18 kultivar melon dan 1 kultivar mentimun. Hasil amplifikasi menggunakan penanda molekular Simple Sequence Repeat (SSR) dengan primer spesifik yaitu CMTA170a menunjukkan pita berukuran 120 bp sedangkan dengan primer CMCT160a+b menunjukkan pita berukuran 212 bp. Hasil yang diuji menunjukkan bahwa semua kultivar melon memiliki gen ketahanan terhadap gummy stem blight baik homozigot maupun heterozigot.

scholarly journals The Determination of the Genetic Distance of Various Snake Melon Cucumis melo var. flexuosus Cultivars Using Inter Simple Sequence Repeat Technique (ISSR)

2021 ◽  
Vol 34 (1) ◽  
pp. 111-123
Author(s):  
Haidar A. K. Al Khazraji ◽  
Abdulkareem M. Abd ◽  
Abdulla A. Abdulla
Keyword(s):  
Cluster Analysis ◽  
Genetic Distance ◽  
Ethidium Bromide ◽  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Inter Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence

The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var. flexuosus species. The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide. Five primers which gave varied product on the agarose have been selected. Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands. While it has shown 100% polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75%. According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82% and the least percentage shown 14.02% by the primer UBC862. Cluster analysis showed the effect on the variance of the studied cultivars.

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