Electromechanical modeling of the transmembrane potential-dependent cell membrane capacitance

2020 ◽  
Vol 117 (4) ◽  
pp. 043701
Author(s):  
E. Sabri ◽  
S. Lasquellec ◽  
C. Brosseau
Keyword(s):  
Cell Membrane ◽  
Transmembrane Potential ◽  
Membrane Capacitance ◽  
Electromechanical Modeling ◽  
Dependent Cell ◽  
Cell Membrane Capacitance

Regression of LV hypertrophy with captopril normalizes membrane currents in rabbits

1998 ◽  
Vol 275 (4) ◽  
pp. H1216-H1224 ◽  
Author(s):  
Seth J. Rials ◽  
Xiaoping Xu ◽  
Ying Wu ◽  
Roger A. Marinchak ◽  
Peter R. Kowey
Keyword(s):  
Cell Membrane ◽  
Action Potential ◽  
Renal Artery ◽  
Current Density ◽  
Action Potential Duration ◽  
Left Ventricular ◽  
Membrane Current ◽  
Membrane Capacitance ◽  
Membrane Currents ◽  
Cell Membrane Capacitance

Recent studies indicate that regression of left ventricular hypertrophy (LVH) normalizes the in situ electrophysiological abnormalities of the left ventricle. This study was designed to determine whether regression of LVH also normalizes the abnormalities of individual membrane currents. LVH was induced in rabbits by renal artery banding. Single ventricular myocytes from rabbits with LVH at 3 mo after renal artery banding demonstrated increased cell membrane capacitance, prolonged action potential duration, decreased inward rectifier K+ current density, and increased transient outward K+ current density compared with myocytes from age-matched controls. Additional rabbits were randomized at 3 mo after banding to treatment with either vehicle or captopril for an additional 3 mo. Myocytes from LVH rabbits treated with vehicle showed persistent membrane current abnormalities. However, myocytes isolated from LVH rabbits treated with captopril had normal cell membrane capacitance, action potential duration, and membrane current densities. Captopril had no direct effect on membrane currents of either control or LVH myocytes. These data support the hypothesis that the action potential prolongation and membrane current abnormalities of LVH are reversed by regression. Normalization of membrane currents probably explains the reduced vulnerability to ventricular arrhythmia observed in this LVH model after treatment with captopril.

Double-peak signal features in microfluidic impedance flow cytometry enable sensitive measurement of cell membrane capacitance

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4296-4309 ◽  
Author(s):  
Karthik Mahesh ◽  
Manoj Varma ◽  
Prosenjit Sen
Keyword(s):  
Flow Cytometry ◽  
Cell Membrane ◽  
Membrane Capacitance ◽  
Cell Populations ◽  
Double Peak ◽  
Frequency Dependent ◽  
Signal Features ◽  
Highly Sensitive ◽  
Dependent Signal ◽  
Cell Membrane Capacitance

In a microfluidic impedance cytometer with co-planar microelectrodes, frequency-dependent signal features of reactive impedance were found to be highly sensitive to cell membrane capacitance and subsequently used to distinguish cell populations.

scholarly journals A Microfluidic Dielectric Spectroscopy System for Characterization of Biological Cells in Physiological Media

Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 463
Author(s):  
Shide Bakhtiari ◽  
Mohammad K. D. Manshadi ◽  
Amin Mansoorifar ◽  
Ali Beskok
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
Equivalent Circuit ◽  
Dielectric Spectroscopy ◽  
Circuit Model ◽  
Membrane Capacitance ◽  
Electrode Polarization ◽  
Biological Cells ◽  
Cell Membrane Capacitance

Dielectric spectroscopy (DS) is a promising cell screening method that can be used for diagnostic and drug discovery purposes. The primary challenge of using DS in physiological buffers is the electrode polarization (EP) that overwhelms the impedance signal within a large frequency range. These effects further amplify with the miniaturization of the measurement electrodes. In this study, we present a microfluidic system and the associated equivalent circuit models for real-time measurements of cell membrane capacitance and cytoplasm resistance in physiological buffers with 10 s increments. The current device captures several hundreds of biological cells in individual microwells through gravitational settling and measures the system’s impedance using microelectrodes covered with dendritic gold nanostructures. Using PC-3 cells (a highly metastatic prostate cancer cell line) suspended in cell growth media (CGM), we demonstrate stable measurements of cell membrane capacitance and cytoplasm resistance in the device for over 15 min. We also describe a consistent application of the equivalent circuit model, starting from the reference measurements used to determine the system parameters. The circuit model is tested using devices with varying dimensions, and the obtained cell parameters between different devices are nearly identical. Further analyses of the impedance data have shown that accurate cell membrane capacitance and cytoplasm resistance can be extracted using a limited number of measurements in the 5 MHz to 10 MHz range. This will potentially reduce the timescale required for real-time DS measurements below 1 s. Overall, the new microfluidic device can be used for the dielectric characterization of biological cells in physiological buffers for various cell screening applications.

scholarly journals Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics

2017 ◽  
Vol 113 (7) ◽  
pp. 1531-1539 ◽  
Author(s):  
Wenfeng Liang ◽  
Yuliang Zhao ◽  
Lianqing Liu ◽  
Yuechao Wang ◽  
Wen Jung Li ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Capacitance ◽  
Cell Membrane Capacitance ◽  
Optically Induced

scholarly journals A Microfluidic Dielectric Spectroscopy System for Characterization of Biological Cells in Physiological Media

2021 ◽  
Author(s):  
Shide Bakhtiari ◽  
Mohammad K. D. Manshadi ◽  
Amin Mansoorifar ◽  
Ali Beskok
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
Equivalent Circuit ◽  
Dielectric Spectroscopy ◽  
Circuit Model ◽  
Membrane Capacitance ◽  
Electrode Polarization ◽  
Biological Cells ◽  
Cell Membrane Capacitance

Dielectric spectroscopy (DS) is a promising cell screening method that can be used for diagnostic and drug discovery purposes. The primary challenge of using DS in physiological buffers is the electrode polarization (EP) that overwhelms the impedance signal within a large frequency range. These effects further amplify with miniaturization of the measurement electrodes. In this study, we present a microfluidic system and the associated equivalent circuit models for real-time measurements of cell membrane capacitance and cytoplasm resistance in physiological buffers with 10s increments. The current device captures several hundreds of biological cells in individual microwells through gravitational settling and measures the system’s impedance using microelectrodes covered with dendritic gold nanostructures. Using PC-3 cells (a highly metastatic prostate cancer cell line) suspended in cell growth media (CGM), we demonstrate stable measurements of cell membrane capacitance and cytoplasm resistance in the device for over 15 minutes. We also describe a consistent application of the equivalent circuit model, starting from the reference measurements used to determine the system parameters. The circuit model is tested using devices with varying dimensions, and the obtained cell parameters between different devices are nearly identical. Further analyses of the impedance data have shown that accurate cell membrane capacitance and cytoplasm resistance can be extracted using a limited number of measurements in the 5 MHz to 10 MHz range. This will potentially reduce the timescale required for real-time DS measurements below 1s. Overall the new microfluidic device can be used for dielectric characterization of biological cells in physiological buffers for various cell screening applications.

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