scholarly journals Genetic diversity of nectar yeast from Central Java based on RAPD method

2020 ◽  
Author(s):  
B. K. Mahardika ◽  
M. Ilmi
Keyword(s):  
Genetic Diversity ◽  
Central Java ◽  
Rapd Method

KERAGAMAN FENOTIPE DAN GENETIK TIGA VARIETAS KELAPA GENJAH KOPYOR ASAL PATI JAWA TENGAH

2016 ◽  
Vol 21 (1) ◽  
pp. 1 ◽  
Author(s):  
ISMAIL MASKROMO ◽  
ELSJE T. TENDA ◽  
MEITY A. TULALO ◽  
HENGKY NOVARIANTO ◽  
DEWI SUKMA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Biology ◽  
Plant Molecular Biology ◽  
Morphological Diversity ◽  
Local Varieties ◽  
Central Java ◽  
Variety Selection ◽  
Ssr Loci ◽  
Biology Laboratory

<p>ABSTRAK<br />Kelapa Genjah kopyor asal Pati, Jawa Tengah merupakan<br />kekayaaan hayati asli Indonesia dengan nilai ekonomi tinggi. Informasi<br />keragaman genetik kelapa kopyor masih terbatas. Data keragaman<br />morfologi dan genetik diperlukan dalam program pemuliaan kelapa<br />kopyor. Penelitian ini mempelajari keragaman tiga varietas kelapa genjah<br />kopyor asal Pati yang telah dilepas berdasarkan karakter morfologi,<br />kuantitas endosperma, dan keragaman alel marka SSR. Penelitian<br />dilakukan di Pati dan di Laboratorium Plant Molecular Biology,<br />Departemen Agronomi dan Hortikultura, IPB. Evaluasi dilakukan terhadap<br />tiga populasi kelapa Genjah kopyor (hijau, coklat, dan kuning) dengan 30<br />tanaman sampel untuk setiap populasi. Rataan data morfologi digunakan<br />untuk menyusun dendogram. Kuantitas endosperma diamati pada satu<br />buah kelapa kopyor per tanaman yang dievaluasi. Karakteristik<br />endosperma dikelompokkan sesuai kategori yang telah ditetapkan. Untuk<br />setiap populasi, analisis marka dengan lima pasang primer SSR dilakukan<br />pada 10 tanaman sampel. Data yang didapat digunakan untuk menentukan<br />keragaman genetik kelapa Genjah kopyor asal Pati. Hasil pengamatan<br />menunjukkan keragaman morfologis dan alel SSR antar tanaman dalam<br />varietasnya (keragaman intra-varietas) rendah. Sebaliknya, keragaman<br />morfologis dan alel SSR antar varietasnya tinggi. Kuantitas endosperma<br />kelapa Genjah kopyor asal Pati bervariasi antara skor 1–6. Keragaman<br />genetik yang rendah dalam varietas dan tinggi antar ketiga varietas (coklat,<br />hijau, dan kuning) memperkuat pelepasan ketiganya sebagai varietas lokal.<br />Selain itu, keragaman genetik antar tanaman dalam varietas yang rendah<br />mendukung penggunaan ketiga varietas lokal sebagai tetua dalam program<br />perakitan varietas kelapa kopyor unggul baru. Tetua yang dipilih dapat<br />diseleksi intra-varietas berdasarkan persentase buah kopyor per tandan<br />dan skor kuantitas endosperma yang tinggi.<br />Kata kunci: Keragaman morfologis, keragaman intra dan antar varietas,<br />kuantitas endosperma</p><p>ABSTRACT<br />Kopyor dwarf coconuts are mutants from Pati, Central Java having<br />high economic values. However, morphological and genetic diversities of<br />this coconut were still limited. Morphological and genetic diversity data<br />are needed for breeding program. The research objectives were to evaluate<br />intra and inter-specific diversity based on morphology, endosperm<br />quantity, and SSR alleles. Field evaluations were conducted in Pati while<br />laboratory activities were at Plant Molecular Biology Laboratory,<br />Department of Agronomy and Horticulture, IPB. Three populations of<br />kopyor dwarf varieties (brown, green, and yellow) were evaluated. Thirty<br />trees were sampled for each population. The average of morphological<br />data were used to construct cluster analysis. Endosperm quantity was<br />scored (0 – 9) based on a single nut sample. Ten palms were analyzed<br />using five SSR loci for each population and used to determine genetic<br />diversity of populations. Results of observations indicated intra-variety<br />morphological and SSR allele variations among kopyor dwarf was low.<br />However, inter-variety variations were high. The endosperm quantity<br />scores among kopyor dwarf coconut ranged from 1–6. The low intra-<br />variety and high inter-variety variations among the three kopyor dwarf<br />coconut supported their release as different local varieties. Moreover, the<br />low intra-variety phenotypic and genotypic diversities among kopyor<br />brown, green, and yellow dwarf coconut support their use as parents for<br />new and superior kopyor coconut variety development in the future. For<br />such purpose, however, it is necessary to conduct intra-variety selection to<br />identify desirable parents based on high kopyor fruit percentage per bunch<br />and for high kopyor endosperm quantity.<br />Key words: Morphological diversity, intra and inter variety diversities,<br />quantity, endosperm</p>

scholarly journals Keragaman Genetik Kukang Jawa (Nycticebus javanicus) Menggunakan Control Region (D-loop) DNA Mitokondria (mtDNA) (GENETIC DIVERSITY ON JAVAN SLOW LORIS (NYCTICEBUS JAVANICUS) USING OF CONTROL REGION (D-LOOP) mtDNA)

2019 ◽  
Vol 20 (3) ◽  
pp. 360
Author(s):  
Wirdateti Wirdateti ◽  
Hayati Aziza ◽  
Handayani Handayani
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Control Region ◽  
West Java ◽  
Slow Loris ◽  
Genetic Population ◽  
Central Java ◽  
Low Genetic Diversity ◽  
The Status ◽  
D Loop

Javan slow loris (Nycticebus javanicus)  one of the species of the genus Nycticebus is endemic in Java.  Their distribution region  is in West Java and Banten, and also reported to be found in Central Java and East Java, although very rarely.  Status of the species is Critically Endangered due to high levels of poaching, habitat loss and habitat fragmentation, so that the necessary conservation in order to increase the population. For conservation management it is necessary to know the status of genetic resources that play of  role in breeding, then this research is to explore the genetic population of the Javan slow loris  from some locations in West Java. The research objective was to assess the diversity of their current population of Java loris through the control region (D-loop) of mitochondrial DNA (mtDNA). A total of 23 individuals samples from Gunung Halimun Park, Tasikmalaya, Garut, Ciamis, Jember and confiscated at the Rehabilitation Center IAR Bogor were used in this stydy. Specific primers of D-loop are used for loris with a length of 296 bp sequence. The result showed there are only five different sites and formed six haplotypes, each haplotypes only 1-3 nucleotides different. Low genetic diversity is shown as much as 42.96% of individuals show the same sequence or genetic distance (d) = 0 that indicate was monomorf population from different population. The genetic distance of the entire population was 0.003 ± 0:01 (0.3%).

scholarly journals Microsatellites to reveal genetic diversity and to distinguish four mangoes of Tegal District, Central Java, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
ANNUR HIDAYAT ◽  
ENNI SUWARSI RAHAYU ◽  
MUHAMMAD ABDULLAH ◽  
Amin Retnoningsih
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Allele Size ◽  
Central Java ◽  
The People ◽  
Specific Allele ◽  
Microsatellite Alleles ◽  
High Repeatability

Abstract. Hidayat A, Rahayu ES, Abdullah M, Retnoningsih A. 2021. Microsatellites to reveal genetic diversity and to distinguish four mangoes of Tegal District, Central Java, Indonesia. Biodiversitas 22: 3467-3473. The Wirasangka mango is Tegal District’s flora of identity, which requires recognition, and its existence deserves to be preserved. However, based on the morphology of the fruit, most of the people of Tegal still have difficulty distinguishing the wirasangka mango from other mangoes. Molecular markers are needed to ascertain the differences between these mango varieties. Microsatellite DNA is a repeating DNA of stable DNA markers with high repeatability and polymorphism. This study analyzes the level of genetic diversity and microsatellite alleles that can differentiate four mangoes from Tegal District, Central Java, Indonesia, i.e. wirasangka, tengkueh, golek, and okyong. Eleven accessions of six locations in Tegal District were analyzed using ten microsatellite loci. The microsatellite amplification result was separated using electrophoresis in 6% polyacrylamide gel and then visualized with silver dye. A total of 35 microsatellite alleles were found measuring 100-1000 bp, ranging from 1-7 alleles for each locus. The average polymorphism information content (PIC) of 0.54 indicates that genetic diversity is relatively high and informative. Therefore, the microsatellite alleles can be used to differentiate mango varieties. The specific allele characteristic of wirasangka mango accession is locus AJ63516, with an allele size of 600-700bp.

scholarly journals Genetic diversity in seedling populations of Dipterocarpus gracilis in Kecubung Ulolanang Nature Conservation Reserve, Indonesia

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Nesty Pratiwi Romadini ◽  
Sapto Indrioko ◽  
Widiyatno WIDIYATNO ◽  
Eny Faridah ◽  
Yeni Widyana Nurchahyani Ratnaningrum
Keyword(s):  
Genetic Diversity ◽  
Nature Conservation ◽  
Genetic Structure ◽  
Forest Products ◽  
Ex Situ ◽  
Mature Trees ◽  
Central Java ◽  
Conservation Of Genetic Resources ◽  
Non Timber Forest Products ◽  
Genetic Drifts

Abstract. Romadini NP, Indrioko S, Widiyatno, Faridah E, Ratnaningrum YWN. 2021. Genetic diversity in seedling populations of Dipterocarpus gracilis in Kecubung Ulolanang Nature Conservation Reserve, Indonesia. Biodiversitas 22: 1138-1145. Dipterocarpus gracilis Blume is one of the native Dipterocarpus species in Java Island, Indonesia. It has commercial value as timber (wood) and non-timber forest products (oleoresin). This species has been considered vulnerable following the Red List of Threatened Species criteria by The International Union for Conservation of Nature (IUCN). We aimed to study the genetic diversity and genetic structure of seedlings as the natural regenerating population of Dipterocarpus gracilis Blume in The Kecubung Ulolanang Nature Conservation Reserve, Batang District, Central Java Province, Indonesia. The D. gracilis population in this area is distributed in four zones. We observed a total of 137 juvenile seedlings representing all the zones. Isozyme markers detected the genetic diversity of D. gracilis by peroxidase (PRX), esterase (EST), and acid phosphatase (ACP) enzymes. The mean observed heterozygosity of all the zones was lower (HO=0.078) than expected heterozygosity (HE=0.203). Genetic depletion occurred because of genetic drifts and founder effects due to low parental diversity. The genetic structure of seedlings is similar to the consequence of inbreeding. We conclude that the genetic diversity of D. gracilis decreases when mature trees are reduced. This population has essential values ​​in Java Island and should be a priority evaluation in the in-situ and ex-situ conservation of genetic resources.

scholarly journals Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence

2019 ◽  
Vol 20 (6) ◽  
Author(s):  
MUHAMMAD FAUZI ARIF ◽  
GANIES RIZA ARISTYA ◽  
RINA SRI KASIAMDARI
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Dna Amplification ◽  
Molecular Study ◽  
Cleaved Amplified Polymorphic Sequence ◽  
Central Java ◽  
Ctab Method ◽  
Polymorphic Bands ◽  
Caps Markers ◽  
Strawberry Cultivars

Abstract. Arif MF, Aristya GR, Kasiamdari RS. 2019. Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence. Biodiversitas 20: 1721-1728. Banyuroto Village, Magelang, Central Java is the center of strawberry (Fragaria spp.) development and cultivation program. The mild climate makes the location very suitable for developing sub-tropic horticulture such as strawberry. Various kinds of cultivars have been developed as part of the agrotourism program. However, genetic variation research of those cultivars is still rarely done. Therefore, the molecular study of strawberry was conducted to determine the genetic variation using CAPS markers. DNA of Five strawberry cultivars from Banyuroto and seven strawberry cultivars from Indonesian Citrus and Subtropical Fruits Research Institute, Malang were isolated using the CTAB method. DNA amplification was performed by PCR using four pairs of primers named APx, OLP, F3H2, and CTI2. The amplification results were cut with three kinds of restriction endonuclease enzymes named MboI, MluI, and TaqI. Restriction product was used to analyze the genetic variation of twelve cultivars and to construct the dendrogram using MVSP software with the UPGMA algorithm. The result showed that the percentage of polymorphic was 45% from 9 polymorphic bands. Dendrogram result showed that there were four clusters. Cluster A consists of Stroberi Hitam, Cluster B consists of Californica Cultivars, cluster C consists of Osso Grande and Osso Purbalingga cultivars, and cluster D consists of Rosalinda, Deeprose, Dorit, Earlibrite, Tristar, Festival, Brastagi, and Aerut. This research concluded that CAPS markers can be used as a method for study genetic diversity of strawberries.

scholarly journals Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
RESTYKANIA ◽  
Suratman ◽  
ARI PITOYO ◽  
SURANTO
Keyword(s):  
Genetic Diversity ◽  
Management Strategies ◽  
Morphological Diversity ◽  
Morphological Characters ◽  
Similarity Coefficient ◽  
Isozyme Variation ◽  
Esterase Isozyme ◽  
Southeastern Part ◽  
Banding Patterns ◽  
Central Java

Abstract. Restykania, Suratman, Pitoyo A, Suranto. 2019. Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 20: 3024-3032. The genetic diversity among madeira vine accessions from southeastern part of Central Java has not been investigated in detail. Information on genetic diversity and relationship among accessions of this plant will provide important input into determining resourceful management strategies and guiding the improvement of plants through plant breeding programs. The objective of this study was to evaluate variation of morphology and isozyme among madeira vine (Anredera cordifolia (Ten.) Steenis) accessions from southeastern part of Central Java. A total of 13 madeira vine accessions were used for morphological observations and isozyme analysis. Morphological diversity was characterized based on measurements and observations of vegetative structures such as stems, leaves, and aerial tubers. Identification of biochemical markers was done using peroxidase and esterase isozyme systems. A relationship dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis. The analysis of quantitative and qualitative morphological characters revealed that the majority of the tested traits showed variation, indicating that there was variability among the tested madeira vine accessions. Polymorphism was observed using isozyme of peroxidase (i.e. two banding patterns) and esterase (i.e. five banding patterns). Based on the dendrogram at a level of 63 % similarity, it showed distinct separation of 13 madeira vine accessions into two major clusters. Cluster I consisted of nine accessions and the closest relationship was shown between KLT 3 and KLT 1 accessions that had 94 % of similarity coefficient. The remaining four accessions were clustered separately as Cluster II with similarity coefficient of 73 %. Therefore, genetic characterization based on morphological and isozyme markers obtained in this study is valuable to understand genetic variability and relationship among madeira vine accessions in southeastern part of Central Java.

scholarly journals ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA

2014 ◽  
Vol 1 (1) ◽  
pp. 2 ◽  
Author(s):  
Arini Maesaroh ◽  
Adi Amurwanto ◽  
Alice Yuniaty
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Population Control ◽  
Winged Bean ◽  
Plant Leaves ◽  
Psophocarpus Tetragonolobus ◽  
Tropical Plant ◽  
Gamma Ray Irradiation ◽  
And Control ◽  
Rapd Method

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

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