scholarly journals The relationship between the three-dimensional structure of porous GaN distributed Bragg reflectors and their birefringence

2020 ◽  
Vol 127 (19) ◽  
pp. 193101
Author(s):  
P. H. Griffin ◽  
K. M. Patel ◽  
T. Zhu ◽  
R. M. Langford ◽  
V. S. Kamboj ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Bragg Reflectors ◽  
Distributed Bragg Reflectors ◽  
Three Dimensional Structure ◽  
The Relationship

scholarly journals Heavy rain and strong wind events and cyclones in the Balearics

2006 ◽  
Vol 7 ◽  
pp. 73-77 ◽  
Author(s):  
J. Campins ◽  
A. Jansà ◽  
A. Genovés
Keyword(s):  
Three Dimensional ◽  
Geographical Location ◽  
Heavy Rain ◽  
Balearic Islands ◽  
Dimensional Structure ◽  
Strong Wind ◽  
Affected Area ◽  
Three Dimensional Structure ◽  
Wind Events ◽  
The Relationship

Abstract. The relationship between heavy rain (HR) and/or strong wind (SW) events and cyclones is investigated for the Balearic Islands. First, a list of HR and SW events is cross-referenced with an objective cyclone database for a 9-year period (from June 1995 to May 2004). The presence of a cyclone centre close to the Balearics is looked for each event. For HR events in most of the cases a cyclone centre is located in the vicinity. Furthermore, cyclones are located in such a way that allow the supply of warm and wet air to the affected area. But for SW events, although in the majority of cases a cyclone centre is detected, cyclones are located farther than for HR events and their geographical location is more widespread. Afterwards, the three-dimensional structure of cyclones related to HR and/or SW events is studied in detail.

Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity

2000 ◽  
Vol 28 (6) ◽  
pp. 680-681 ◽  
Author(s):  
M. W. Hayman ◽  
T. Fawcett ◽  
T. F. Schierer ◽  
J. W. Simon ◽  
J. T. M. Kroon ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Recombinant Enzyme ◽  
Dimensional Structure ◽  
Substrate Selectivity ◽  
Cucurbita Moschata ◽  
Three Dimensional Structure ◽  
Overexpression System ◽  
The Relationship

In an attempt to rationalize the relationship between structure and substrate selectivity of glycerol-3-phosphate acyltransferase (GPAT, 1AT, EC 2.3.1.15) we have cloned a number of cDNAs into the PET overexpression system using a PCR-based approach. Following assay of the recombinant enzyme we noted that the substrate selectivity of the squash (Cucurbita moschata) enzyme had altered dramatically. This form of GPAT has now been crystallized and its full three-dimensional structure elucidated. Since we now have two forms of the enzyme that display different substrate selectivities this should provide a powerful tool to determine the basis of the selectivity changes. Kinetic and structural analyses are currently being performed to rationalize the changes which have taken place.

The Three-Dimensional Structure of Swirl-Stabilized Flames in a Lean Premixed Multinozzle Can Combustor

2015 ◽  
Vol 138 (3) ◽  
Author(s):  
Janith Samarasinghe ◽  
Stephen J. Peluso ◽  
Bryan D. Quay ◽  
Domenic A. Santavicca
Keyword(s):  
Gas Turbine ◽  
Tomographic Reconstruction ◽  
Flame Structure ◽  
Combustion Process ◽  
Three Dimensional ◽  
Static Stability ◽  
Dimensional Structure ◽  
Reconstruction Technique ◽  
Three Dimensional Structure ◽  
The Relationship

Flame structure can have a significant effect on a combustor's static stability (resistance to blowoff) and dynamic stability (combustion instability) and therefore is an important aspect of the combustion process that must be taken into account in the design of gas turbine combustors. While the relationship between flame structure and flame stability has been studied extensively in single-nozzle combustors, relatively few studies have been conducted in multinozzle combustor configurations typical of actual gas turbine combustion systems. In this paper, a chemiluminescence-based tomographic reconstruction technique is used to obtain three-dimensional images of the flame structure in a laboratory-scale five-nozzle can combustor. Analysis of the 3D images reveals features of the complex, three-dimensional structure of this multinozzle flame. Effects of interacting swirling flows, flame–flame interactions, and flame–wall interactions on the flame structure are also discussed.

The Three-dimensional structure of frozen-hydrated nudaurelia capensis β virus

1987 ◽  
Vol 45 ◽  
pp. 650-651
Author(s):  
N. H. Olson ◽  
T. S. Baker ◽  
Wu Bo Mu ◽  
J. E. Johnson ◽  
D. A. Hendry
Keyword(s):  
South African ◽  
Rna Virus ◽  
Carbon Film ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Electron Dose ◽  
Total Electron ◽  
Three Dimensional Structure ◽  
Negative Stain ◽  
Dimensional Reconstruction

Nudaurelia capensis β virus (NβV) is an RNA virus of the South African Pine Emperor moth, Nudaurelia cytherea capensis (Lepidoptera: Saturniidae). The NβV capsid is a T = 4 icosahedron that contains 60T = 240 subunits of the coat protein (Mr = 61,000). A three-dimensional reconstruction of the NβV capsid was previously computed from visions embedded in negative stain suspended over holes in a carbon film. We have re-examined the three-dimensional structure of NβV, using cryo-microscopy to examine the native, unstained structure of the virion and to provide a initial phasing model for high-resolution x-ray crystallographic studiesNβV was purified and prepared for cryo-microscopy as described. Micrographs were recorded ∼1 - 2 μm underfocus at a magnification of 49,000X with a total electron dose of about 1800 e-/nm2.

Three Dimensional structure and organization of diploid chromosomes by optical section microscopy

1987 ◽  
Vol 45 ◽  
pp. 633-635
Author(s):  
David A. Agard ◽  
Yasushi Hiraoka ◽  
John W. Sedat
Keyword(s):  
Dimensional Space ◽  
Three Dimensional ◽  
Developmental State ◽  
Mitotic Cell ◽  
Biological Properties ◽  
Dimensional Structure ◽  
Specific Gene ◽  
Three Dimensional Structure ◽  
Complementary Techniques ◽  
Dynamic Structures

In an effort to understand the complex relationship between structure and biological function within the nucleus, we have embarked on a program to examine the three-dimensional structure and organization of Drosophila melanogaster embryonic chromosomes. Our overall goal is to determine how DNA and proteins are organized into complex and highly dynamic structures (chromosomes) and how these chromosomes are arranged in three dimensional space within the cell nucleus. Futher, we hope to be able to correlate structual data with such fundamental biological properties as stage in the mitotic cell cycle, developmental state and transcription at specific gene loci.Towards this end, we have been developing methodologies for the three-dimensional analysis of non-crystalline biological specimens using optical and electron microscopy. We feel that the combination of these two complementary techniques allows an unprecedented look at the structural organization of cellular components ranging in size from 100A to 100 microns.

Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution

1990 ◽  
Vol 48 (1) ◽  
pp. 282-283
Author(s):  
José L. Carrascosa ◽  
José M. Valpuesta ◽  
Hisao Fujisawa
Keyword(s):  
Dna Sequences ◽  
Expression Vector ◽  
Three Dimensional ◽  
Deae Cellulose ◽  
Dna Packaging ◽  
Dimensional Structure ◽  
Two Dimensional ◽  
Freezing And Thawing ◽  
Three Dimensional Structure ◽  
Dimensional Reconstruction

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

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