scholarly journals Mechanical properties of crosslinks controls failure mechanism of hierarchical intermediate filament networks

2012 ◽  
Vol 2 (1) ◽  
pp. 014005 ◽  
Author(s):  
Zhao Qin ◽  
Markus J. Buehler
Keyword(s):  
Mechanical Properties ◽  
Failure Mechanism ◽  
Intermediate Filament ◽  
Filament Networks

scholarly journals Mechanical Properties of Vimentin Intermediate Filament Networks

2014 ◽  
Vol 106 (2) ◽  
pp. 785a
Author(s):  
Huayin Wu ◽  
Mikkel Jensen ◽  
Ming Guo ◽  
David A. Weitz
Keyword(s):  
Mechanical Properties ◽  
Intermediate Filament ◽  
Filament Networks ◽  
Vimentin Intermediate Filament

Intermediate Filament Cytoskeletal System: Dynamic and Mechanical Properties

1998 ◽  
Vol 194 (3) ◽  
pp. 361-363 ◽  
Author(s):  
R. D. Goldman ◽  
S. Clement ◽  
S. Khuon ◽  
R. Moir ◽  
A. Trejo-Skalli ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Intermediate Filament ◽  
System Dynamic ◽  
Cytoskeletal System

Paranemin and the organization of desmin filament networks

2001 ◽  
Vol 114 (6) ◽  
pp. 1079-1089 ◽  
Author(s):  
S.C. Schweitzer ◽  
M.W. Klymkowsky ◽  
R.M. Bellin ◽  
R.M. Robson ◽  
Y. Capetanaki ◽  
...  
Keyword(s):  
Cell Lines ◽  
Intermediate Filament ◽  
Transgene Expression ◽  
De Novo ◽  
Muscle Cells ◽  
The Other ◽  
Intermediate Filament Proteins ◽  
Mouse Fibroblasts ◽  
Filament Networks ◽  
Filament Network

De novo expression of vimentin, GFAP or peripherin leads to the assembly of an extended intermediate filament network in intermediate filament-free SW13/cl.2 cells. Desmin, in contrast, does not form extended filament networks in either SW13/cl.2 or intermediate filament-free mouse fibroblasts. Rather, desmin formed short thickened filamentous structures and prominent spot-like cytoplasmic aggregates that were composed of densely packed 9–11 nm diameter filaments. Analysis of stably transfected cell lines indicates that the inability of desmin to form extended networks is not due to a difference in the level of transgene expression. Nestin, paranemin and synemin are large intermediate filament proteins that coassemble with desmin in muscle cells. Although each of these large intermediate filament proteins colocalized with desmin when coexpressed in SW-13 cells, expression of paranemin, but not synemin or nestin, led to the formation of an extended desmin network. A similar rescue of desmin network organization was observed when desmin was coexpressed with vimentin, which coassembles with desmin, or with keratins, which formed a distinct filament network. These studies demonstrate that desmin filaments differ in their organizational properties from the other vimentin-like intermediate filament proteins and appear to depend upon coassembly with paranemin, at least when they are expressed in non-muscle cells, in order to form an extended filament network.

scholarly journals Study on Mechanical Properties and Failure Mechanism of Axial Braided C/C Composite

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Chunguang Wang ◽  
Weiping Tian ◽  
Min Tang
Keyword(s):  
Mechanical Properties ◽  
Failure Mechanism ◽  
Fiber Bundle ◽  
Strain Curve ◽  
Axial Direction ◽  
Radial Compression ◽  
Compression Sample ◽  
Transverse Fiber ◽  
Failure Morphology ◽  
Axial Fracture

In order to study the mechanical properties and failure mechanism of the axial braided C/C composites, the microscopic and macroscopic mechanical properties of the composite were investigated. In view of the size effect of the samples, the properties of the samples with different thickness were tested. The strain during loading was measured by optical method, and the failure morphology was observed by SEM. The changing characteristics of stress-strain curve were analyzed, and the failure characteristics of materials and failure mechanism under various loads were obtained. It was found that brittle fracture was observed during the tensile process of axial braided C/C composites, and the main failure forms were fiber rod pulling and partial fiber rod breaking in the axial direction. Radial failure was mainly in the form of fiber bundle fracture and crack stratification propagation. When compressed, the material exhibited pseudoplastic characteristics. The radial compression sample was cut along a 45-degree bevel. The axial compression curve was in the form of double fold, the axial fiber rod was unstable, and the transverse fiber bundle was cut. During in-plane shearing, the axial fracture was brittle and the fiber rod was cut. The radial direction showed the fracture and pulling of the fiber bundle, and the material had the characteristics of pseudoplasticity. The research methods and results in this paper could provide important references for the optimization and rational application of C/C composite materials.

scholarly journals The focal adhesion scaffold protein Hic-5 regulates vimentin organization in fibroblasts

2019 ◽  
Vol 30 (25) ◽  
pp. 3037-3056 ◽  
Author(s):  
Rishel B. Vohnoutka ◽  
Anushree C. Gulvady ◽  
Gregory Goreczny ◽  
Kyle Alpha ◽  
Samuel K. Handelman ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Actin Cytoskeleton ◽  
Western Blotting ◽  
Focal Adhesion ◽  
Posttranslational Modifications ◽  
Intermediate Filament ◽  
Fluorescence Recovery After Photobleaching ◽  
Scaffold Protein ◽  
Cancer Associated Fibroblasts ◽  
Genetic Ablation

Focal adhesion (FA)-stimulated reorganization of the F-actin cytoskeleton regulates cellular size, shape, and mechanical properties. However, FA cross-talk with the intermediate filament cytoskeleton is poorly understood. Genetic ablation of the FA-associated scaffold protein Hic-5 in mouse cancer-associated fibroblasts (CAFs) promoted a dramatic collapse of the vimentin network, which was rescued following EGFP-Hic-5 expression. Vimentin collapse correlated with a loss of detergent-soluble vimentin filament precursors and decreased vimentin S72/S82 phosphorylation. Additionally, fluorescence recovery after photobleaching analysis indicated impaired vimentin dynamics. Microtubule (MT)-associated EB1 tracking and Western blotting of MT posttranslational modifications indicated no change in MT dynamics that could explain the vimentin collapse. However, pharmacological inhibition of the RhoGTPase Cdc42 in Hic-5 knockout CAFs rescued the vimentin collapse, while pan-formin inhibition with SMIFH2 promoted vimentin collapse in Hic-5 heterozygous CAFs. Our results reveal novel regulation of vimentin organization/dynamics by the FA scaffold protein Hic-5 via modulation of RhoGTPases and downstream formin activity.

scholarly journals Failure Mechanism of a Coal-Rock Combined Body with Inclinations of Structural Planes and a Calculation Model for Impact Energy

2019 ◽  
Vol 2019 ◽  
pp. 1-18
Author(s):  
Yi-Chao Zhao ◽  
Ming-Shi Gao ◽  
Yong-Liang He ◽  
Dong Xu
Keyword(s):  
Mechanical Properties ◽  
Failure Mechanism ◽  
Impact Energy ◽  
Coal Mines ◽  
Calculation Model ◽  
Engineering Practice ◽  
Test Results ◽  
Failure Characteristics ◽  
Coal Rock ◽  
Rock Strata

A coal-rock (CR) combined body can be used to simulate structures of coal and rock strata, and its impact-induced failure characteristic conforms more close to engineering practice. Exploring the mechanical properties and impact energy in a CR combined body contributes to better predictions of rock bursts in coal mines. In the study, the mechanical properties of CR combined bodies with four different inclinations (0°, 15°, 30°, and 45°) of structural planes were measured, and also their failure mechanism was analysed. Based on the theory of particle mechanics, a calculation model for impact energy in a CR combined body with inclinations was established and then verified by using monitored acoustic emission (AE) data. The test results showed that inclination affected mechanical properties and failure characteristics of the CR combined body, i.e., the larger the inclination, the lower the strength and impact energy in the CR combined body and the lower the level of damage. The proposed calculation model for impact energy revealed the mechanical essence of energy accumulation and release of a CR combined body, providing a reference for investigating rock burst in coal mines.

scholarly journals Mechanical Properties and Failure Mechanism of Sandstone with Mudstone Interlayer

2019 ◽  
Vol 136 ◽  
pp. 04048
Author(s):  
Chengxiang Wang ◽  
Huailiang Kou ◽  
Wei Han
Keyword(s):  
Mechanical Properties ◽  
Failure Mechanism ◽  
Class Ii ◽  
Class I ◽  
Class Iii ◽  
Uniaxial Test ◽  
Irregular Distribution ◽  
Distribution Form ◽  
Layered Rock Mass ◽  
Rock Mass Failure

In order to study the strength and failure characteristics of sandstone with mudstone interlayer. According to mudstone interlayer dip and distribution form, it can be divided into three kinds of sandstone: sandstone with horizontal mudstone interlayer is Class I; sandstone with inclined mudstone interlayer is Class II; sandstone with irregular distribution mudstone is Class III. The three kinds of sandstone uniaxial test were carried out and combined with the theory of layered rock mass failure, it revealed mechanical properties and failure mechanism of sandstone with mudstone interlayer. The results show that: crack generated from interface in Class I and II and crack generated from irregular distribution mudstone interlayer in Class III; The sandstone with higher strength is destroyed before the mudstone interlayer; sandstone at unbonded interface is failure before sandstone at bonded interface; Three kinds of sandstone vary in compressive strength, Class II is greater than Class III while Class III is greater than Class I.

scholarly journals Properties of Intermediate Filament Networks Assembled from Keratin 8 and 18 in the Presence of Mg2+

2012 ◽  
Vol 103 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Anke Leitner ◽  
Tobias Paust ◽  
Othmar Marti ◽  
Paul Walther ◽  
Harald Herrmann ◽  
...  
Keyword(s):  
Intermediate Filament ◽  
Keratin 8 ◽  
Filament Networks
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