Hydrophobic surfaces help reduce harmful protein fibril formation

Scilight ◽  
2020 ◽  
Vol 2020 (36) ◽  
pp. 361110
Author(s):  
Savannah Mandel
Keyword(s):  
Fibril Formation ◽  
Hydrophobic Surfaces ◽  
Protein Fibril

scholarly journals Adsorption of unfolded Cu/Zn superoxide dismutase onto hydrophobic surfaces catalyzes its formation of amyloid fibrils

2019 ◽  
Vol 32 (2) ◽  
pp. 77-85
Author(s):  
Mohammad Ashhar I Khan ◽  
Ulrich Weininger ◽  
Sven Kjellström ◽  
Shashank Deep ◽  
Mikael Akke
Keyword(s):  
Superoxide Dismutase ◽  
Surface Area ◽  
Amyloid Fibrils ◽  
Hydrophobic Surface ◽  
Fibril Formation ◽  
Hydrophobic Surfaces ◽  
Molecular Features ◽  
Denaturant Concentration

Abstract Intracellular aggregates of superoxide dismutase 1 (SOD1) are associated with amyotrophic lateral sclerosis. In vivo, aggregation occurs in a complex and dense molecular environment with chemically heterogeneous surfaces. To investigate how SOD1 fibril formation is affected by surfaces, we used an in vitro model system enabling us to vary the molecular features of both SOD1 and the surfaces, as well as the surface area. We compared fibril formation in hydrophilic and hydrophobic sample wells, as a function of denaturant concentration and extraneous hydrophobic surface area. In the presence of hydrophobic surfaces, SOD1 unfolding promotes fibril nucleation. By contrast, in the presence of hydrophilic surfaces, increasing denaturant concentration retards the onset of fibril formation. We conclude that the mechanism of fibril formation depends on the surrounding surfaces and that the nucleating species might correspond to different conformational states of SOD1 depending on the nature of these surfaces.

The Sulfate Moieties of Glycosaminoglycans Are Critical for the Enhancement of β-Amyloid Protein Fibril Formation

2001 ◽  
Vol 72 (4) ◽  
pp. 1681-1687 ◽  
Author(s):  
Gerardo M. Castillo ◽  
Widia Lukito ◽  
Thomas N. Wight ◽  
Alan D. Snow
Keyword(s):  
Fibril Formation ◽  
Amyloid Protein ◽  
Β Amyloid ◽  
Protein Fibril

Type IV collagen prevents amyloid β-protein fibril formation

Life Sciences ◽  
2002 ◽  
Vol 70 (13) ◽  
pp. 1555-1564 ◽  
Author(s):  
Yoichi Kiuchi ◽  
Yoshihiko Isobe ◽  
Kiyomi Fukushima
Keyword(s):  
Type Iv Collagen ◽  
Amyloid Β ◽  
Fibril Formation ◽  
Amyloid Β Protein ◽  
Type Iv ◽  
Β Protein ◽  
Protein Fibril

Kinetics of protein fibril formation: Methods and mechanisms

2017 ◽  
Vol 100 ◽  
pp. 3-10 ◽  
Author(s):  
E. Kiran Kumar ◽  
Neshatul Haque ◽  
N. Prakash Prabhu
Keyword(s):  
Fibril Formation ◽  
Protein Fibril ◽  
Kinetics Of

Entactin-induced inhibition of human amyloid β-protein fibril formation in vitro

2001 ◽  
Vol 305 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Yoichi Kiuchi ◽  
Yoshihiko Isobe ◽  
Kiyomi Fukushima
Keyword(s):  
Amyloid Β ◽  
Fibril Formation ◽  
Amyloid Β Protein ◽  
Β Protein ◽  
Protein Fibril

scholarly journals Mechanisms of Protein Fibril Formation in Amyloid Beta and Lysozyme Proteins

2019 ◽  
Vol 116 (3) ◽  
pp. 195a-196a
Author(s):  
Carlos M. Perez ◽  
Ghanim Ullah ◽  
Tatiana Miti ◽  
Martin Muschol
Keyword(s):  
Amyloid Beta ◽  
Fibril Formation ◽  
Protein Fibril
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