Structural similarity and lifetimes of turbulence structures in fully developed pipe flow

1987 ◽  
Vol 30 (10) ◽  
pp. 3006 ◽  
Author(s):  
K. J. Bullock ◽  
R. E. Cooper ◽  
R. E. Kronauer ◽  
J. C. S. Lai
2007 ◽  
Vol 129 (11) ◽  
pp. 1415-1421 ◽  
Author(s):  
Joseph Borowsky ◽  
Timothy Wei

An experimental investigation of a two-phase pipe flow was undertaken to study kinematic and dynamic parameters of the fluid and solid phases. To accomplish this, a two-color digital particle image velocimetry and accelerometry (DPIV∕DPIA) methodology was used to measure velocity and acceleration fields of the fluid phase and solid phase simultaneously. The simultaneous, two-color DPIV∕DPIA measurements provided information on the changing characteristics of two-phase flow kinematic and dynamic quantities. Analysis of kinematic terms indicated that turbulence was suppressed due to the presence of the solid phase. Dynamic considerations focused on the second and third central moments of temporal acceleration for both phases. For the condition studied, the distribution across the tube of the second central moment of acceleration indicated a higher value for the solid phase than the fluid phase; both phases had increased values near the wall. The third central moment statistic of acceleration showed a variation between the two phases with the fluid phase having an oscillatory-type profile across the tube and the solid phase having a fairly flat profile. The differences in second and third central moment profiles between the two phases are attributed to the inertia of each particle type and its response to turbulence structures. Analysis of acceleration statistics provides another approach to characterize flow fields and gives some insight into the flow structures, even for steady flows.


AIAA Journal ◽  
1989 ◽  
Vol 27 (3) ◽  
pp. 283-292 ◽  
Author(s):  
J. C. S. Lai ◽  
K. J. Bullock ◽  
R. E. Kronauer

1978 ◽  
Vol 88 (3) ◽  
pp. 585-608 ◽  
Author(s):  
K. J. Bullock ◽  
R. E. Cooper ◽  
F. H. Abernathy

The paper describes correlation measurements in both broad and narrow frequency bands of the longitudinal velocity fluctuations in fully developed pipe flow at four positions for a reference probe whilst a second probe was traversed radially from deep in the sublayer to a position near the axis with both longitudinal and transverse separations zero (Δx = Δz = 0). Such measurements require that both the Covariant (Co) and Quadrature (Quad) correlations be determined for each of the 15 frequencies used to constrain the wave component λx.The new data demonstrate that low frequency, large scale turbulence fluctuations extend over the majority of the radial region and that these components are highly correlated. By using a similarity variable kxy, along with a normalized wall distance y/y REF, both correlation functions, i.e. the Co and the Quad components, are shown to collapse. The physical significance of this is discussed.The broad-band data do not collapse because of the large range of wave sizes. However, the present experiment does show that strong radial correlations exist even when one probe is at y+ = 1. This conflicts with the earlier data of Favre, but agrees with the more recent work of Comte-Bellot. There is a significant amount of turbulent energy in frequencies less than 16 Hz (ω+ = 0·008) for turbulent flows of about 105 Reynolds number.The spectral function ωΦ(ω) is also presented for a range of y+ values. Using this form for the power spectral density, along with the stochastic wave modelling and similarity arguments of this paper, it is shown how a consistent explanation for the behaviour of these spectra is obtained. In addition some preliminary results from cross-spectral analyses are presented and suggestions made as to their physical significance.


Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.


Author(s):  
M. Boublik ◽  
R.M. Wydro ◽  
W. Hellmann ◽  
F. Jenkins

Ribosomes are ribonucleoprotein particles necessary for processing the genetic information of mRNA into proteins. Analogy in composition and function of ribosomes from diverse species, established by biochemical and biological assays, implies their structural similarity. Direct evidence obtained by electron microscopy seems to be of increasing relevance in understanding the structure of ribosomes and the mechanism of their role in protein synthesis.The extent of the structural homology between prokaryotic and eukaryotic ribosomes has been studied on ribosomes of Escherichia coli (E.c.) and Artemia salina (A.s.). Despite the established differences in size and in the amount and proportion of ribosomal proteins and RNAs both types of ribosomes show an overall similarity. The monosomes (stained with 0.5% aqueous uranyl acetate and deposited on a fine carbon support) appear in the electron micrographs as round particles with a diameter of approximately 225Å for the 70S E.c. (Fig. 1) and 260Å for the 80S A.s. monosome (Fig. 2).


Author(s):  
M. Boublik ◽  
N. Robakis ◽  
W. Hellmann ◽  
F. Jenkins

Ribosomes are ribonucleoprotein particles which process the genetic information coded in mRNA into protein synthesis. The analogy in function and composition of ribosomes from various sources, both prokaryotic and eukaryo-tic, imply a structural similarity. At present, high resolution electron microscopy is the most direct technique with a potential to resolve the extent of the structural homology of ribosomal particles at a macromolecular level. The structure of ribosomes is highly complex as a result of the large number of their constituents. In general, 80S eukaryotic monosomes consist of two uneven subunits - large (60S) and small (40S) - accomodating four different RNAs and approximately 80 different proteins. Mutual orientation of both subunits on the monosome is of particular interest because it determines the interface, the supposed site of interactions of ribosomes with other macro-molecules involved in peptide bond formation. Since entrapping of the contrasting solution (0.5% aqueous uranyl acetate) obscures all structural details in the interface, information on its architecture is limited to an indirect reconstruction based on the established 3-D structure of both sub-units and their mutual position after association.


2019 ◽  
Vol 476 (24) ◽  
pp. 3835-3847 ◽  
Author(s):  
Aliyath Susmitha ◽  
Kesavan Madhavan Nampoothiri ◽  
Harsha Bajaj

Most Gram-positive bacteria contain a membrane-bound transpeptidase known as sortase which covalently incorporates the surface proteins on to the cell wall. The sortase-displayed protein structures are involved in cell attachment, nutrient uptake and aerial hyphae formation. Among the six classes of sortase (A–F), sortase A of S. aureus is the well-characterized housekeeping enzyme considered as an ideal drug target and a valuable biochemical reagent for protein engineering. Similar to SrtA, class E sortase in GC rich bacteria plays a housekeeping role which is not studied extensively. However, C. glutamicum ATCC 13032, an industrially important organism known for amino acid production, carries a single putative sortase (NCgl2838) gene but neither in vitro peptide cleavage activity nor biochemical characterizations have been investigated. Here, we identified that the gene is having a sortase activity and analyzed its structural similarity with Cd-SrtF. The purified enzyme showed a greater affinity toward LAXTG substrate with a calculated KM of 12 ± 1 µM, one of the highest affinities reported for this class of enzyme. Moreover, site-directed mutation studies were carried to ascertain the structure functional relationship of Cg-SrtE and all these are new findings which will enable us to perceive exciting protein engineering applications with this class of enzyme from a non-pathogenic microbe.


Author(s):  
Valeriy G. Yakubenko ◽  
Anna L. Chultsova

Identification of water masses in areas with complex water dynamics is a complex task, which is usually solved by the method of expert assessments. In this paper, it is proposed to use a formal procedure based on the application of the method of optimal multiparametric analysis (OMP analysis). The data of field measurements obtained in the 68th cruise of the R/V “Academician Mstislav Keldysh” in the summer of 2017 in the Barents Sea on the distribution of temperature, salinity, oxygen, silicates, nitrogen, and phosphorus concentration are used as a data for research. A comparison of the results with data on the distribution of water masses in literature based on expert assessments (Oziel et al., 2017), allows us to conclude about their close structural similarity. Some differences are related to spatial and temporal shifts of measurements. This indicates the feasibility of using the OMP analysis technique in oceanological studies to obtain quantitative data on the spatial distribution of different water masses.


2013 ◽  
Vol 50 (2) ◽  
pp. 258-265
Author(s):  
Pál Hegedűs

In this paper we analyse the natural permutation module of an affine permutation group. For this the regular module of an elementary Abelian p-group is described in detail. We consider the inequivalent permutation modules coming from nonconjugate complements. We prove their strong structural similarity well exceeding the fact that they have equal Brauer characters.


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