Surface enhanced infrared absorption (SEIRA) observed on different metal surface structures

1998 ◽  
Author(s):  
H. D. Wanzenböck ◽  
N. Weissenbacher ◽  
R. Kellner
2002 ◽  
Vol 22 (5-6) ◽  
pp. 541-547 ◽  
Author(s):  
Alexander A. Kamnev ◽  
Lev A. Dykman ◽  
Petros A. Tarantilis ◽  
Moschos G. Polissiou

Using surface-enhanced infrared absorption (SEIRA) spectroscopy of dry films of colloidal gold (CG) bioconjugates with protein A, it is shown that certain characteristic bands of the protein (e.g., amide I, amide II and some other vibration modes) are essentially affected by the metal surface. Thus, the method may be used for controlling the quality of such bioconjugates. Moreover, it is demonstrated that the biospecific reaction of protein A attached to CG particles with human immunoglobulin G (IgG) results in further essential changes in SEIRA spectra, providing a means for an easy and rapid IR spectroscopic detection of biospecific immunochemical interactions (i.e., spectroimmunochemistry). The results obtained can form a basis for developing test systems for detecting various biospecific interactions.


2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yufang Niu ◽  
Jie Yao ◽  
Keyu Wang

Nickel nanoparticles were electrochemically deposited on indium-tin oxide (ITO) coated glass plate in a modified Watt’s electrolyte. The surface-enhanced infrared absorption (SEIRA) effect of the nanoparticles was evaluated by attenuated total reflection spectroscopy (ATR-FTIR) using o-nitroaniline as a probe molecule. Electrodeposition parameters such as deposition time, pH value, and the type of surfactants were investigated. The morphology and the microstructure of the deposits were characterized by the field emission scanning electron microscope (FESEM) and the atomic force microscope (AFM), respectively. The results indicate that the optimum parameters were potential of 1.3 V, time of 30 s, and pH of 8.92 in the solution of 0.3756 mol/L diethanolamine, 0.1 mol/L nickel sulfate, 0.01 mol/L nickel chloride, and 0.05 mol/L boric acid. The FESEM observation shows that the morphology of nickel nanoparticles with best enhancement effect is spherical and narrowly distributed particles with the average size of 50 nm. SEIRA enhancement factor is about 68.


Author(s):  
Wei-Chuan Shih ◽  
Fusheng Zhao ◽  
Ousamma Zenasni ◽  
Masud Arnob ◽  
Yulung Sung

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