scholarly journals Cito-compability analysis of mesenchymal stem cells in platelet rich fibrin matrix (PRFM) for tissue regeneration

2019 ◽  
Author(s):  
Gita Pratama ◽  
Budi Wiweko ◽  
Normalina Sandora ◽  
Evanti Kusumawardhani ◽  
Deniswari Rahayu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Platelet Rich Fibrin ◽  
Fibrin Matrix

scholarly journals INCUBATION OF PLATELET-RICH FIBRIN MATRIX WITH MESENCHYMAL STEM CELLS IMPROVES MATRIX STIFFNESS

2020 ◽  
pp. 111-116
Author(s):  
MIRTA H. REKSODIPUTRO ◽  
GITA PRATAMA ◽  
BUDI WIWEKO ◽  
EVANTHI KUSUMAWARDHANI ◽  
DENISWARI RAHAYU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Platelet Rich Plasma ◽  
Testing Machine ◽  
Cell Types ◽  
Biomechanical Properties ◽  
Biomechanical Property ◽  
Human Umbilical Cord ◽  
Platelet Rich Fibrin ◽  
Fibrin Matrix

Objective: The platelet-rich fibrin matrix (PRFM) is condensed platelet-rich plasma (PRP) and should possess a comparable biomechanical property to the transplanted sites, for them to be physiologically functional. The aim of this study was to investigate the effect of human bone marrow mesenchymal stem cells (hBM-MSC) or human umbilical cord mesenchymal stem cells (hUC-MSC) on the biomechanical properties of PRFM. Methods: PRFM was prepared by the gelation of PRP using 25 mmol CaCl2. The resulting coin-shaped PRFM pellets, 5 cm in diameter and 300 μm thick, were directly seeded with hUC-MSC or hBM-MSC at 2,000 cells cm-2, followed by 24 h incubation at 37 °C in 5% (v/v) CO2 in air. The samples were then observed by scanning electron microscopy to determine the morphology of the matrix surface. The PRFM biomechanical properties were determined at a 10 mm. min-1failure rate using an MCT 2150 universal testing machine (AandD Co. LTD). Results: SEM imaging of the surface of the PRFM seeded with hBM-MSC and hUC-MSC showed a cloudy layer that thickened over time. The elastin slope of the PRFM was significantly improved after seeding with hBM-MSC and hUC-MSC when compared with unseeded PRFM (p<0.002, R2=0.983). Both cell types elicited similar biomechanical effects (p=0.99). Conclusion: PRFM seeded with hBM-MSC or hUC-MSC showed significantly increased elasticity.

EVALUATION OF BONE TISSUE REGENERATION USING A COMBINATION OF AUTOLOGOUS FIBRIN MATRIX WITH PRE-DIFFERENTIATED MESENCHYMAL STEM CELLS IN AN EXPERIMENTAL MODEL

2019 ◽  
Vol 25 (3) ◽  
pp. 172-175
Author(s):  
Igor S. Kopetsky ◽  
V. V. Loginov ◽  
S. D. Ryzhova ◽  
P. S. Virgilyev ◽  
A. V. Lokhonina ◽  
...  
Keyword(s):  
Stem Cells ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Damage Zone ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Facial Skeleton ◽  
Fibrin Matrix

Introduction. According to domestic and foreign authors, the frequency of damage to the mandible bone is 36-84% of all damage to the bones of the facial skeleton. Since A.Y. Friedenstein made a key contribution to the study of mesenchymal stem cells (MSC), their analysis, types, methods of isolation and use in various combinations with materials, allowed to expand the boundaries of regenerative medicine. Aim of the study. The purpose of the study is to evaluate the regenerative potential of cortical bone tissue in the region of the mandible angle, with the introduction of exogenous pre-differentiated MSC (MSCs) on the autologous fibrin matrix in the experiment. Material and methods. We carried out an experimental study on 27 rabbits of the Soviet Chinchilla breed. Animals were divided into 3 groups: A, B and control. The bone defect diameter of 6mm was formed in the angle of the mandible of all animals with a trepan. Groups A and B mandible defects were injected with autologous fibrin clot with pre-differentiated MSCs in the amount of 300 thousand and 1 million cells, respectively. Defect size was assessed after 8, 10 and 12 weeks. Results. At week 12 after surgery, in the experimental group B the size of the bone tissue defect was significantly reduced and amounted to 0.006022 ± 0.00135 mm3, while in the control group the defect volume was 0.05597 ± 0.00505 mm3, and in group A was 0.02235 ± 0.0056 mm3. In an experimental study, an improvement was obtained in the pathophysiological processes of bone tissue regeneration under conditions of a bicortical defect with minimal presence of spongy substance in the damage zone.

scholarly journals A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal stem cells

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sevda Pouraghaei Sevari ◽  
Sahar Ansari ◽  
Alireza Moshaverinia
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
3D Structure ◽  
Three Dimensional ◽  
Scientific Data ◽  
Clinical Practices ◽  
Human Teeth ◽  
Innovative Strategies ◽  
Local Recruitment

AbstractTissue engineering approaches have emerged recently to circumvent many limitations associated with current clinical practices. This elegant approach utilizes a natural/synthetic biomaterial with optimized physiomechanical properties to serve as a vehicle for delivery of exogenous stem cells and bioactive factors or induce local recruitment of endogenous cells for in situ tissue regeneration. Inspired by the natural microenvironment, biomaterials could act as a biomimetic three-dimensional (3D) structure to help the cells establish their natural interactions. Such a strategy should not only employ a biocompatible biomaterial to induce new tissue formation but also benefit from an easily accessible and abundant source of stem cells with potent tissue regenerative potential. The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs) with self-renewing and multilineage differentiation capabilities. In the current review article, we seek to highlight recent progress and future opportunities in dental MSC-mediated therapeutic strategies for tissue regeneration using two possible approaches, cell transplantation and cell homing. Altogether, this paper develops a general picture of current innovative strategies to employ dental-derived MSCs combined with biomaterials and bioactive factors for regenerating the lost or defective tissues and offers information regarding the available scientific data and possible applications.

scholarly journals Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1078
Author(s):  
Han Young Kim ◽  
Suk Ho Bhang
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Therapeutic Agent ◽  
Therapeutic Effects ◽  
Hydrodynamic Size ◽  
Regeneration Strategy ◽  
Neuroprotective Effects ◽  
The Poor

As a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted MSCs remains challenging. Although MSC-derived exosomes were recently introduced and reported to have great potential to replace conventional MSC-based therapeutics, the poor production yield and heterogeneity of exosomes are critical hurdles for their further applications. Herein, we report the fabrication of exosome-mimetic MSC-engineered nanovesicles (MSC-NVs) by subjecting cells to serial extrusion through filters. The fabricated MSC-NVs exhibit a hydrodynamic size of ~120 nm, which is considerably smaller than the size of MSCs (~30 μm). MSC-NVs contain both MSC markers and exosome markers. Importantly, various therapeutic growth factors originating from parent MSCs are encapsulated in the MSC-NVs. The MSC-NVs exerted various therapeutic effects comparable to those of MSCs. They also significantly induced the angiogenesis of endothelial cells and showed neuroprotective effects in damaged neuronal cells. The results collectively demonstrate that the fabricated MSC-NVs can serve as a nanosized therapeutic agent for tissue regeneration.

Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells

2014 ◽  
Vol 40 (6) ◽  
pp. 679-689 ◽  
Author(s):  
Shahram Ghanaati ◽  
Patrick Booms ◽  
Anna Orlowska ◽  
Alica Kubesch ◽  
Jonas Lorenz ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
B Lymphocytes ◽  
Tissue Regeneration ◽  
Distal Part ◽  
T And B Lymphocytes ◽  
Cell Detection ◽  
Neutrophilic Granulocytes ◽  
Platelet Rich Fibrin ◽  
Study Protocols

Choukroun's platelet-rich fibrin (PRF) is obtained from blood without adding anticoagulants. In this study, protocols for standard platelet-rich fibrin (S-PRF) (2700 rpm, 12 minutes) and advanced platelet-rich fibrin (A-PRF) (1500 rpm, 14 minutes) were compared to establish by histological cell detection and histomorphometrical measurement of cell distribution the effects of the centrifugal force (speed and time) on the distribution of cells relevant for wound healing and tissue regeneration. Immunohistochemistry for monocytes, T and B -lymphocytes, neutrophilic granulocytes, CD34-positive stem cells, and platelets was performed on clots produced from four different human donors. Platelets were detected throughout the clot in both groups, although in the A-PRF group, more platelets were found in the distal part, away from the buffy coat (BC). T- and B-lymphocytes, stem cells, and monocytes were detected in the surroundings of the BC in both groups. Decreasing the rpm while increasing the centrifugation time in the A-PRF group gave an enhanced presence of neutrophilic granulocytes in the distal part of the clot. In the S-PRF group, neutrophils were found mostly at the red blood cell (RBC)-BC interface. Neutrophilic granulocytes contribute to monocyte differentiation into macrophages. Accordingly, a higher presence of these cells might be able to influence the differentiation of host macrophages and macrophages within the clot after implantation. Thus, A-PRF might influence bone and soft tissue regeneration, especially through the presence of monocytes/macrophages and their growth factors. The relevance and feasibility of this tissue-engineering concept have to be proven through in vivo studies.

Advanced platelet-rich fibrin extract treatment promotes the proliferation and differentiation of Human Adipose-Derived Mesenchymal Stem Cells through activation of tryptophan metabolism

2021 ◽  
Vol 16 ◽  
Author(s):  
Guan-Ming Lu ◽  
Li-Yuan Jiang ◽  
Dong-Lin Huang ◽  
Yong-Xian Rong ◽  
Yang-Hong Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regulatory Network ◽  
Expression Profiles ◽  
Differential Expression Analysis ◽  
Tryptophan Metabolism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Platelet Rich Fibrin ◽  
The Core ◽  
Proliferation And Differentiation

Background: Advanced platelet-rich fibrin extract (APRFE) contains a high concentration of various cytokines that are helpful for improving stem cells repair function. Objective: However, the underlying mechanism of APRFE improving stem cell repairing is not clear. Methods: We produced APRFE by centrifuging fresh peripheral blood samples and isolated and identified human adipose-derived mesenchymal stem cells (ADMSCs). The abundance of cytokines contained in APRFE was detected by the Enzyme-linked immunosorbent assay (ELISA). The ADMSCs treated with or without APRFE were collected for transcriptome sequencing. Results: Based on the sequencing data, the expression profiles were contracted. The differentially expressed genes and lncRNA (DEGs and DElncRNAs) were obtained using for the differential expression analysis. The lncRNA-miRNA-mRNA network was constructed based on the miRNet database. The further enrichment analysis results showed that the biological functions were mainly related to proliferation, differentiation, and cell-cell function. To explore the role of APRFE, the protein-protein interaction network was constructed among the cytokines included in APRFE and DEGs. Furthermore, we constructed the global regulatory network based on the RNAInter and TRRUST database. The pathways in the global regulatory network were considered as the core pathways. We found that the DEGs in the core pathways were associated with stemness scores. Conclusion: In summary, we predicted that APRFE activated three pathways (tryptophan metabolism, mTOR signaling pathway, and adipocytokine signaling) to promote the proliferation and differentiation of ADMSCs. The finding may be helpful for guiding the application of ADMSCs in the clinic.

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