Electrokinetic transport of a non-conducting liquid droplet in a polyelectrolyte medium

2020 ◽  
Vol 32 (1) ◽  
pp. 012011
Author(s):  
Sirsendu Sekhar Barman ◽  
Somnath Bhattacharyya
Keyword(s):  
Liquid Droplet ◽  
Electrokinetic Transport ◽  
Conducting Liquid

Study on heat transfer characteristics of liquid droplet radiator: With VS without inter-droplets coupling

2021 ◽  
Vol 156 ◽  
pp. 108199
Author(s):  
Di Huang ◽  
Kewei Ning ◽  
Fulong Zhao ◽  
Jian Deng ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Liquid Droplet ◽  
Heat Transfer Characteristics ◽  
Transfer Characteristics

scholarly journals C. elegans germ granules require both assembly and localized regulators for mRNA repression

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Scott Takeo Aoki ◽  
Tina R. Lynch ◽  
Sarah L. Crittenden ◽  
Craig A. Bingman ◽  
Marvin Wickens ◽  
...  
Keyword(s):  
Liquid Droplet ◽  
Scaffolding Protein ◽  
P Granules ◽  
C Elegans ◽  
Cytoplasmic Rna ◽  
And Function ◽  
Rnp Granules ◽  
Key Residues ◽  
Reporter Mrna

AbstractCytoplasmic RNA–protein (RNP) granules have diverse biophysical properties, from liquid to solid, and play enigmatic roles in RNA metabolism. Nematode P granules are paradigmatic liquid droplet granules and central to germ cell development. Here we analyze a key P granule scaffolding protein, PGL-1, to investigate the functional relationship between P granule assembly and function. Using a protein–RNA tethering assay, we find that reporter mRNA expression is repressed when recruited to PGL-1. We determine the crystal structure of the PGL-1 N-terminal region to 1.5 Å, discover its dimerization, and identify key residues at the dimer interface. Mutations of those interface residues prevent P granule assembly in vivo, de-repress PGL-1 tethered mRNA, and reduce fertility. Therefore, PGL-1 dimerization lies at the heart of both P granule assembly and function. Finally, we identify the P granule-associated Argonaute WAGO-1 as crucial for repression of PGL-1 tethered mRNA. We conclude that P granule function requires both assembly and localized regulators.

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