Bone-like multilevel calcium phosphate coating modulates an interaction of mesenchymal stem cells and tumor cells

2019 ◽  
Author(s):  
V. V. Malashchenko ◽  
E. O. Shunkin ◽  
V. V. Shupletsova ◽  
O. G. Khaziakhmatova ◽  
K. A. Yurova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Tumor Cells ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating

scholarly journals Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating

2020 ◽  
Vol 21 (20) ◽  
pp. 7682
Author(s):  
Larisa Litvinova ◽  
Kristina Yurova ◽  
Valeria Shupletsova ◽  
Olga Khaziakhmatova ◽  
Vladimir Malashchenko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Mrna Expression ◽  
Gene Expression Regulation ◽  
Chemical Properties ◽  
Calcium Phosphate Coating ◽  
Average Roughness

The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (RUNX2, BMP2, BMP6, BGLAP, and ALPL) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.

Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

2009 ◽  
Vol 5 (4) ◽  
pp. 1294-1305 ◽  
Author(s):  
L. Saldaña ◽  
S. Sánchez-Salcedo ◽  
I. Izquierdo-Barba ◽  
F. Bensiamar ◽  
L. Munuera ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Human Mesenchymal Stem Cells

scholarly journals Attachment, Proliferation, and Chondroinduction of Mesenchymal Stem Cells on Porous Chitosan-Calcium Phosphate Scaffolds

2013 ◽  
Vol 7 (1) ◽  
pp. 275-281 ◽  
Author(s):  
Steven Elder ◽  
Anuhya Gottipati ◽  
Hilary Zelenka ◽  
Joel Bumgardner
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Treatment Options ◽  
Preliminary Investigation ◽  
Osteochondral Lesions ◽  
Porous Chitosan ◽  
Tissue Engineering Approach ◽  
Osteochondral Regeneration ◽  
Further Development

Symptomatic osteochondral lesions occur frequently, but relatively few treatment options are currently available. The purpose of this study was to conduct a preliminary investigation into a new tissue engineering approach to osteochondral regeneration. The concept is a biphasic construct consisting of a porous, osteoconductive chitosan-calcium phosphate scaffold supporting a layer of neocartilage formed by marrow-derived mesenchymal stem cells. Two experiments were conducted to assess the feasibility of this approach. The first experiment characterized the attachment efficiency and proliferation of primary human marrow-derived mesenchymal stem cells seeded relatively sparely onto the scaffold’s surface. The second experiment compared two different methods of creating a biphasic construct using a much higher density of primary porcine marrow stromal cells. About 40% of the sparsely seeded human cells attached and proliferated rapidly. Constructs formed by one of the two experimental techniques exhibited a layer of cartilaginous tissue which only partially covered the scaffold’s surface due to inadequate adhesion between the cells and the scaffold. This study demonstrates some potential for the approach to yield an implantable biphasic construct, but further development is required to improve cell-scaffold adhesion.

Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

2009 ◽  
Vol 631-632 ◽  
pp. 211-216 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
T. Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

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