scholarly journals Selective ablation of cancer cells with low intensity pulsed ultrasound

2020 ◽  
Vol 116 (1) ◽  
pp. 013701 ◽  
Author(s):  
David R. Mittelstein ◽  
Jian Ye ◽  
Erika F. Schibber ◽  
Ankita Roychoudhury ◽  
Leyre Troyas Martinez ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Selective Ablation

Regulation of exosomes secretion by low-intensity pulsed ultrasound in lung cancer cells

2019 ◽  
Vol 383 (1) ◽  
pp. 111448 ◽  
Author(s):  
Qingyu Zeng ◽  
Shibin Hong ◽  
Xue Wang ◽  
Yirui Cheng ◽  
Junfeng Sun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Effects of low-intensity pulsed ultrasound on osteosarcoma and cancer cells

2012 ◽  
Vol 28 (2) ◽  
pp. 481-486 ◽  
Author(s):  
YASUSHI SAWAI ◽  
HIROAKI MURATA ◽  
KAZUTAKA KOTO ◽  
TAKAAKI MATSUI ◽  
NAOYUKI HORIE ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Selective Ablation of Cancer Cells with Low Intensity Pulsed Ultrasound

2019 ◽  
Author(s):  
David R. Mittelstein ◽  
Jian Ye ◽  
Erika F. Schibber ◽  
Ankita Roychoudhury ◽  
Leyre Troyas Martinez ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Intensity ◽  
Focused Ultrasound ◽  
Mechanical Energy ◽  
Pulsed Ultrasound ◽  
High Intensity Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Specific Frequency ◽  
Acoustic Standing Waves

ABSTRACTUltrasound can be focused into deep tissues with millimeter precision to perform non-invasive ablative therapy for diseases such as cancer. In most cases, this ablation uses high intensity ultrasound to deposit non-selective thermal or mechanical energy at the ultrasound focus, damaging both healthy bystander tissue and cancer cells. Here we describe an alternative low intensity pulsed ultrasound approach that leverages the distinct mechanical properties of neoplastic cells to achieve inherent cancer selectivity. We show that when applied at a specific frequency and pulse duration, focused ultrasound selectively disrupts a panel of breast, colon, and leukemia cancer cell models in suspension without significantly damaging healthy immune or red blood cells. Mechanistic experiments reveal that the formation of acoustic standing waves and the emergence of cell-seeded cavitation lead to cytoskeletal disruption, expression of apoptotic markers, and cell death. The inherent selectivity of this low intensity pulsed ultrasound approach offers a potentially safer and thus more broadly applicable alternative to non-selective high intensity ultrasound ablation.

scholarly journals Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

2021 ◽  
Vol 6 (11) ◽  
pp. 4073-4082
Author(s):  
Kunzhan Cai ◽  
Yilai Jiao ◽  
Quan Quan ◽  
Yulin Hao ◽  
Jie Liu ◽  
...  
Keyword(s):  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Transcriptional Control ◽  
Target Genes ◽  
Pulsed Ultrasound ◽  
Activated T Cells ◽  
In Vivo Models ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

Sign in / Sign up

Export Citation Format

Share Document