High bacterial inactivation on the aqueous brass surface for clinical trials by cold atmospheric plasma

2019 ◽  
Vol 90 (10) ◽  
pp. 104105
Author(s):  
Yi Yu
Keyword(s):  
Clinical Trials ◽  
Atmospheric Plasma ◽  
Bacterial Inactivation ◽  
Cold Atmospheric Plasma ◽  
Brass Surface

Cold Atmospheric Plasma Decontamination of the Pericarps of Fruit

2008 ◽  
Vol 71 (2) ◽  
pp. 302-308 ◽  
Author(s):  
STEFANO PERNI ◽  
DAVID W. LIU ◽  
GILBERT SHAMA ◽  
MICHAEL G. KONG
Keyword(s):  
Detection Limit ◽  
Operating Conditions ◽  
Atmospheric Plasma ◽  
Resistant Organism ◽  
Efficient Production ◽  
Operating Voltage ◽  
Bacterial Inactivation ◽  
Cold Atmospheric Plasma ◽  
Cell Counts ◽  
Oxygen Atoms

This investigation describes the inactivation by cold atmospheric plasmas of one pathogenic and three spoilage organisms on the pericarps of mangoes and melons. The operating voltage necessary for efficient microbial decontamination of fruit pericarps was first established using Escherichia coli at a concentration of 107 CFU/cm2 on the surface of mango. It was found that, when the plasma was sustained slightly above its breakdown voltage of 12 kV (peak to peak), no inactivation was detected when cells were plated onto tryptone soya extract agar (TSA). However, when plated onto eosin methylene blue agar, sublethal injury corresponding to approximately 1 log reduction was achieved, whereas on TSA supplemented with 4% NaCl a greater reduction of 1.5 log was revealed. When the voltage was increased by 33% to 16 kV, a reduction in cell counts of 3 log was achieved on all three plating media. Further investigations at these new operating conditions were conducted using a range of spoilage microorganisms (Saccharomyces cerevisae, Pantoea agglomerans, and Gluconacetobacter liquefaciens) all at a surface concentration of 106 CFU/cm2 on the pericarps of mango and melon. P. agglomerans and G. liquefaciens were reduced below the detection limit (corresponding to 3 log) after only 2.5 s on both fruits, whereas E. coli required 5 s to reach the same level of inactivation. S. cerevisae was the most resistant organism studied and was reduced in numbers below the detection limit after 10 s on mango and 30 s on melon. The optical emission spectra generated by the cold atmospheric plasma at both high and low operating voltages were compared in order to identify putative lethal species. It was shown that an increase in the applied voltage led to more efficient production of reactive plasma species, particularly oxygen atoms, and the production of oxygen atoms was related to the level of bacterial inactivation achieved. Production of atomic oxygen could be used as an indicator of inactivation efficiency for scaling up cold plasma systems for whole fruit.

scholarly journals Cold Atmospheric Plasma in Dentistry

2016 ◽  
Vol 1 (2) ◽  
pp. 82-86
Author(s):  
Rajesh S Nair ◽  
Betty Babu ◽  
Eeshan Mushtaq
Keyword(s):  
Atmospheric Plasma ◽  
Natural Phenomenon ◽  
Light Sources ◽  
Bacterial Inactivation ◽  
Fusion Reactions ◽  
Heavy Particles ◽  
Cold Atmospheric Plasma ◽  
Aurora Borealis ◽  
Nuclear Fusion Reactions ◽  
Tissue Alteration

ABSTRACT Introduction Plasma is the fourth state of matter and others are liquid, gas, and solid. Plasma occurs as a natural phenomenon in the universe and appears in the form of fire, in the polar aurora borealis and in the nuclear fusion reactions of the sun. It can be produced artificially which has gained importance in the fields of plasma screens or light sources. Plasma is of two types: Thermal and nonthermal or cold atmospheric plasma (CAP). Thermal plasma has electrons and heavy particles (ions and neutral) at the same temperature. Cold atmospheric plasma is said to be nonthermal as it has electron at a hotter temperature than the heavy particles that are at room temperature. Cold atmospheric plasma is a specific type of plasma, i.e., <104°F at the point of application. It could become a new and painless method to prepare cavities for restoration with improved longevity. Also it is capable of bacterial inactivation and noninflammatory tissue alteration, which makes it an attractive tool for the treatment of dental caries and for composite restorations. Plasma can also be used for tooth whitening. This review focuses on some dental application of plasma. How to cite this article Nair RS, Babu B, Mushtaq E. Cold Atmospheric Plasma in Dentistry. J Oper Dent Endod 2016;1(2):82-86.

Formation of ammonium in saline solution treated by nanosecond pulsed cold atmospheric microplasma: a route to fast inactivation of E. coli bacteria

RSC Advances ◽  
2015 ◽  
Vol 5 (52) ◽  
pp. 42135-42140 ◽  
Author(s):  
Simon Maheux ◽  
David Duday ◽  
Thierry Belmonte ◽  
Christian Penny ◽  
Henry-Michel Cauchie ◽  
...  
Keyword(s):  
Saline Solution ◽  
Atmospheric Plasma ◽  
Main Process ◽  
Bacterial Inactivation ◽  
Fast Inactivation ◽  
Cold Atmospheric Plasma ◽  
E Coli ◽  
Solution Treated ◽  
Saline Solutions ◽  
First Time

The formation of significant NH4+species in saline solutions treated by He/N2cold atmospheric plasma is proposed for the first time as the main process responsible for the fast bacterial inactivation ofE. coliat ambient temperature and physiological pH.

scholarly journals Combined Antimicrobial Effect of Bio-Waste Olive Leaf Extract and Remote Cold Atmospheric Plasma Effluent

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1890
Author(s):  
Jose Gustavo De la Ossa ◽  
Hani El Kadri ◽  
Jorge Gutierrez-Merino ◽  
Thomas Wantock ◽  
Thomas Harle ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Preliminary Investigation ◽  
Atmospheric Plasma ◽  
Aureus Strain ◽  
Bacterial Inactivation ◽  
Olive Leaf ◽  
Cold Atmospheric Plasma ◽  
E Coli ◽  
Olive Leaf Extract

A novel strategy involving Olive Leaf Extract (OLE) and Cold Atmospheric Plasma (CAP) was developed as a green antimicrobial treatment. Specifically, we reported a preliminary investigation on the combined use of OLE + CAP against three pathogens, chosen to represent medical and food industries (i.e., E. coli, S. aureus and L. innocua). The results indicated that a concentration of 100 mg/mL (total polyphenols) in OLE can exert an antimicrobial activity, but still insufficient for a total bacterial inactivation. By using plain OLE, we significantly reduced the growth of Gram positive S. aureus and L. innocua, but not Gram-negative E. coli. Instead, we demonstrated a remarkable decontamination effect of OLE + CAP in E. coli, S. aureus and L. innocua samples after 6 h. This effect was optimally maintained up to 24 h in S. aureus strain. E. coli and L. innocua grew again in 24 h. In the latter strain, OLE alone was most effective to significantly reduce bacterial growth. By further adjusting the parameters of OLE + CAP technology, e.g., OLE amount and CAP exposure, it could be possible to prolong the initial powerful decontamination over a longer time. Since OLE derives from a bio-waste and CAP is a non-thermal technology based on ionized air, we propose OLE + CAP as a potential green platform for bacterial decontamination. As a combination, OLE and CAP can lead to better antimicrobial activity than individually and may replace or complement conventional thermal procedures in food and biomedical industries.

scholarly journals Use of cold-atmospheric plasma in oncology: a concise systematic review

2018 ◽  
Vol 10 ◽  
pp. 175883591878647 ◽  
Author(s):  
Antoine Dubuc ◽  
Paul Monsarrat ◽  
François Virard ◽  
Nofel Merbahi ◽  
Jean-Philippe Sarrette ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Trials ◽  
Cell Lines ◽  
Pharmaceutical Products ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Physical Parameters ◽  
Cold Atmospheric Plasma

Background: Cold-atmospheric plasma (CAP) is an ionized gas produced at an atmospheric pressure. The aim of this systematic review is to map the use of CAP in oncology and the implemented methodologies (cell targets, physical parameters, direct or indirect therapies). Methods: PubMed, the International Clinical Trials Registry Platform and Google Scholar were explored until 31 December 2017 for studies regarding the use of plasma treatment in oncology ( in vitro, in vivo, clinical trials). Results: 190 original articles were included. Plasma jets are the most-used production systems (72.1%). Helium alone was the most-used gas (35.8%), followed by air (26.3%) and argon (22.1%). Studies were mostly in vitro (94.7%) and concerned direct plasma treatments (84.2%). The most targeted cancer cell lines are human cell lines (87.4%), in particular, in brain cancer (16.3%). Conclusions: This study highlights the multiplicity of means of production and clinical applications of the CAP in oncology. While some devices may be used directly at the bedside, others open the way for the development of new pharmaceutical products that could be generated at an industrial scale. However, its clinical use strongly needs the development of standardized reliable protocols, to determine the more efficient type of plasma for each type of cancer, and its combination with conventional treatments.

Targeting Protective Catalase of Tumor Cells with Cold Atmospheric Plasma- Activated Medium (PAM)

2018 ◽  
Vol 18 (6) ◽  
pp. 784-804 ◽  
Author(s):  
Georg Bauer
Keyword(s):  
Control System ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Plasma Potential ◽  
Atmospheric Plasma ◽  
Antitumor Action ◽  
Active Principle ◽  
Reaction Products ◽  
Cold Atmospheric Plasma

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

The Application of the Cold Atmospheric Plasma-Activated Solutions in Cancer Treatment

2018 ◽  
Vol 18 (6) ◽  
pp. 769-775 ◽  
Author(s):  
Dayun Yan ◽  
Jonathan H. Sherman ◽  
Michael Keidar
Keyword(s):  
Cancer Treatment ◽  
Atmospheric Plasma ◽  
Current Status ◽  
Cold Atmospheric Plasma ◽  
Anti Cancer ◽  
Long Time ◽  
Key Topics ◽  
The Common

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

scholarly journals Tiny Cold Atmospheric Plasma Jet for Biomedical Applications

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 249
Author(s):  
Zhitong Chen ◽  
Richard Obenchain ◽  
Richard E. Wirz
Keyword(s):  
Biomedical Applications ◽  
Plasma Jet ◽  
Discharge Voltage ◽  
Plasma Jets ◽  
Atmospheric Plasma ◽  
Physical Parameters ◽  
Cold Atmospheric Plasma ◽  
Water Metal ◽  
Plasma Probe ◽  
Jet Nozzle

Conventional plasma jets for biomedical applications tend to have several drawbacks, such as high voltages, high gas delivery, large plasma probe volume, and the formation of discharge within the organ. Therefore, it is challenging to employ these jets inside a living organism’s body. Thus, we developed a single-electrode tiny plasma jet and evaluated its use for clinical biomedical applications. We investigated the effect of voltage input and flow rate on the jet length and studied the physical parameters of the plasma jet, including discharge voltage, average gas and subject temperature, and optical emissions via spectroscopy (OES). The interactions between the tiny plasma jet and five subjects (de-ionized (DI) water, metal, cardboard, pork belly, and pork muscle) were studied at distances of 10 mm and 15 mm from the jet nozzle. The results showed that the tiny plasma jet caused no damage or burning of tissues, and the ROS/RNS (reactive oxygen/nitrogen species) intensity increased when the distance was lowered from 15 mm to 10 mm. These initial observations establish the tiny plasma jet device as a potentially useful tool in clinical biomedical applications.

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