scholarly journals An electrochemiluminescent sensing matrix for real-time probing of cell-output reactive oxygen species

2019 ◽  
Vol 13 (4) ◽  
pp. 044115
Author(s):  
Chen Fang ◽  
Dan Du ◽  
Dongning Li ◽  
Jie Huang ◽  
Yifeng Tu
Keyword(s):  
Reactive Oxygen Species ◽  
Real Time ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Sensing Matrix ◽  
Electrochemiluminescent Sensing

scholarly journals Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe

2018 ◽  
Vol 264 ◽  
pp. 419-425 ◽  
Author(s):  
Zhangjun Hu ◽  
Guanqing Yang ◽  
Jiwen Hu ◽  
Hui Wang ◽  
Peter Eriksson ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Real Time ◽  
Fluorescent Probe ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Reactive oxygen species-triggered off-on fluorescence donor for imaging hydrogen sulfide delivery in living cells

2019 ◽  
Vol 10 (33) ◽  
pp. 7690-7694 ◽  
Author(s):  
Yiming Hu ◽  
Xiaoyi Li ◽  
Yu Fang ◽  
Wen Shi ◽  
Xiaohua Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Sulfide ◽  
Real Time ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
The Real ◽  
Real Time Imaging

A reactive oxygen species-triggered off-on fluorescence H2S donor is develop for the real-time imaging of H2S delivery and the cytoprotection against the hazardous oxidative environment.

Real-time monitoring of peroxynitrite (ONOO−) in the rat brain by developing a ratiometric electrochemical biosensor

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 2150-2157 ◽  
Author(s):  
Feiyue Liu ◽  
Hui Dong ◽  
Yang Tian
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Rat Brain ◽  
Real Time ◽  
Superoxide Anion ◽  
Electrochemical Biosensor ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Real Time Monitoring ◽  
The Brain

As a reactive oxygen species (ROS), peroxynitrite (ONOO−) generated by nitric oxide (NO) and superoxide anion (O2˙−) plays important roles in physiological and pathological processes in the brain.

scholarly journals Reactive oxygen species formation during tetanic contractions in single isolated Xenopus myofibers

2011 ◽  
Vol 111 (3) ◽  
pp. 898-904 ◽  
Author(s):  
Li Zuo ◽  
Leonardo Nogueira ◽  
Michael C. Hogan
Keyword(s):  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Real Time ◽  
Contractile Activity ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Oxygen Species ◽  
Fatigue Development ◽  
Species Formation ◽  
Significant Difference

Contracting skeletal muscle produces reactive oxygen species (ROS) that have been shown to affect muscle function and adaptation. However, real-time measurement of ROS in contracting myofibers has proven to be difficult. We used amphibian ( Xenopus laevis) muscle to test the hypothesis that ROS are formed during contractile activity in isolated single skeletal muscle fibers and that this contraction-induced ROS formation affects fatigue development. Single myofibers were loaded with 5 μM dihydrofluorescein-DA (Hfluor-DA), a fluorescent probe that reacts with ROS and results in the formation of fluorescein (Fluor) to precisely monitor ROS generation within single myofibers in real time using confocal miscroscopy. Three identical periods of maximal tetanic contractions (1 contraction/3 s for 2 min, separated by 60 min of rest) were conducted by each myofiber ( n = 6) at 20°C. Ebselen (an antioxidant) was present in the perfusate (10 μM) during the second contractile period. Force was reduced by ∼30% during each of the three contraction periods, with no significant difference in fatigue development among the three periods. The Fluor signal, indicative of ROS generation, increased significantly above baseline in both the first (42 ± 14%) and third periods (39 ± 10%), with no significant difference in the increase in fluorescence between the first and third periods. There was no increase of Fluor in the presence of ebselen during the second contractile period. These results demonstrated that, in isolated intact Xenopus myofibers, 1) ROS can be measured in real time during tetanic contractions, 2) contractile activity induced a significant increase above resting levels of ROS production, and 3) ebselen treatment reduced ROS generation to baseline levels but had no effect on myofiber contractility and fatigue development.

scholarly journals Non-invasive real-time imaging of reactive oxygen species (ROS) using multispectral auto-fluorescence imaging technique: a novel tool for redox biology

2020 ◽  
Author(s):  
Abbas Habibalahi ◽  
Mahdieh Dashtbani Moghari ◽  
Jared M. Campbell ◽  
Ayad G. Anwer ◽  
Saabah B. Mahbub ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Real Time ◽  
Spectral Range ◽  
Imaging Technique ◽  
Critical Role ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Redox Biology ◽  
Non Invasive ◽  
Entire Spectral Range

AbstractDetecting reactive oxygen species (ROS) that play a critical role as redox modulators and signalling molecules in biological systems currently requires invasive methods such as ROS - specific indicators for imaging and quantification. We developed a non-invasive, real-time, label-free imaging technique for assessing the level of ROS in live cells and thawed cryopreserved tissues that is compatible with in-vivo imaging. The technique is based on autofluorescence multispectral imaging (AFMI) carried out in an adapted fluorescence microscope with an expanded number of spectral channels spanning specific excitation (365 nm-495 nm) and emission (420 nm-700 nm) wavelength ranges. We established a strong quantitative correlation between the spectral information obtained from AFMI and the level of ROS obtained from CellROX staining. The results were obtained in several cell types (HeLa, PANC1 and mesenchymal stem cells) and in live kidney tissue. Additioanly, two spectral regimes were considered: with and without UV excitation (wavelengths > 400 nm); the latter being suitable for UV-sensitive systems such as the eye. Data were analyzed by linear regression combined with an optimization method of swarm intelligence. This allowed the calibration of AFMI signals to the level of ROS with excellent correlation (R= 0.84, p=0.00) in the entire spectral range and very good correlation (R= 0.78, p=0.00) in the limited, UV-free spectral range. We also developed a strong classifier which allowed us to distinguish moderate and high levels of ROS in these two regimes (AUC= 0.91 in the entire spectral range and AUC = 0.78 for UV-free imaging). These results indicate that ROS in cells and tissues can be imaged non-invasively, which opens the way to future clinical applications in conditions where reactive oxygen species are known to contribute to progressive disease such as in ophthalmology, diabetes, kidney disease, cancer and neurodegenerative diseases.

Sign in / Sign up

Export Citation Format

Share Document