Efficacy of propolis fluoride in inhibiting the formation of Streptoccocus mutans, Streptococcus gordonii, and Streptococcus sanguinis biofilm

2019 ◽  
Author(s):  
Sri Angky Soekanto ◽  
Saly Salim Alatas ◽  
Rima Ristanti ◽  
Ferry P. Gultom ◽  
Muhamad Sahlan
Keyword(s):  
Streptococcus Gordonii ◽  
Streptococcus Sanguinis ◽  
Mutans Streptococcus

scholarly journals Eficácia antimicrobiana de ionômeros de vidro restauradores contra espécies bacterianas iniciadoras do biofilme dental e cariogênicas

2019 ◽  
Author(s):  
Rafael Nobrega Stipp ◽  
Fernanda Felipe de Andrade ◽  
Laura da Silva Gabriel ◽  
Marina Stênico ◽  
Gabriela Castro Fonseca ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Streptococcus Gordonii ◽  
Streptococcus Sanguinis ◽  
Mutans Streptococcus

Os fluoretos liberados dos ionômeros de vidro possuem atividade antimicrobiana, o que contribui para a eficácia do material. O objetivo deste estudo foi avaliar a atividade antimicrobiana de 18 ionômeros convencionais restauradores de vidro contra Streptococcus mutans, Streptococcus gordonii e Streptococcus sanguinis. Foram avaliados a capacidade de inibição do crescimento bacteriano na presença do ionômero e de redução da queda do pH, ocasionada pelos microrganismos estudados. Os iônomeros apresentaram atividade variada em relação aos microrganismos estudados.

scholarly journals Streptococcus gordonii Hsa Environmentally Constrains Competitive Binding by Streptococcus sanguinis to Saliva-Coated Hydroxyapatite

2007 ◽  
Vol 189 (8) ◽  
pp. 3106-3114 ◽  
Author(s):  
Angela H. Nobbs ◽  
Yongshu Zhang ◽  
Ali Khammanivong ◽  
Mark C. Herzberg
Keyword(s):  
Selective Advantage ◽  
Competitive Binding ◽  
Tooth Surface ◽  
Oral Streptococci ◽  
Streptococcus Gordonii ◽  
Streptococcus Sanguinis ◽  
Competitive Capabilities ◽  
Vitro Model ◽  
Isogenic Mutants

ABSTRACT Competition between pioneer colonizing bacteria may determine polymicrobial succession during dental plaque development, but the ecological constraints are poorly understood. For example, more Streptococcus sanguinis than Streptococcus gordonii organisms are consistently isolated from the same intraoral sites, yet S. gordonii fails to be excluded and survives as a species over time. To explain this observation, we hypothesized that S. gordonii could compete with S. sanguinis to adhere to saliva-coated hydroxyapatite (sHA), an in vitro model of the tooth surface. Both species bound similarly to sHA, yet 10- to 50-fold excess S. gordonii DL1 reduced binding of S. sanguinis SK36 by 85 to >95%. S. sanguinis, by contrast, did not significantly compete with S. gordonii to adhere. S. gordonii competed with S. sanguinis more effectively than other species of oral streptococci and depended upon the salivary film on HA. Next, putative S. gordonii adhesins were analyzed for contributions to interspecies competitive binding. Like wild-type S. gordonii, isogenic mutants with mutations in antigen I/II polypeptides (sspAB), amylase-binding proteins (abpAB), and Csh adhesins (cshAB) competed effectively against S. sanguinis. By contrast, an hsa-deficient mutant of S. gordonii showed significantly reduced binding and competitive capabilities, while these properties were restored in an hsa-complemented strain. Thus, Hsa confers a selective advantage to S. gordonii over S. sanguinis in competitive binding to sHA. Hsa expression may, therefore, serve as an environmental constraint against S. sanguinis, enabling S. gordonii to persist within the oral cavity, despite the greater natural prevalence of S. sanguinis in plaque and saliva.

scholarly journals Streptococcal Antagonism in Oral Biofilms: Streptococcus sanguinis and Streptococcus gordonii Interference with Streptococcus mutans

2008 ◽  
Vol 190 (13) ◽  
pp. 4632-4640 ◽  
Author(s):  
Jens Kreth ◽  
Yongshu Zhang ◽  
Mark C. Herzberg
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Species ◽  
Ecological Factors ◽  
Oral Streptococci ◽  
Streptococcus Gordonii ◽  
Interspecies Interactions ◽  
Streptococcus Sanguinis ◽  
Production Of Hydrogen ◽  
Oral Biofilms ◽  
Competence System

ABSTRACT Biofilms are polymicrobial, with diverse bacterial species competing for limited space and nutrients. Under healthy conditions, the different species in biofilms maintain an ecological balance. This balance can be disturbed by environmental factors and interspecies interactions. These perturbations can enable dominant growth of certain species, leading to disease. To model clinically relevant interspecies antagonism, we studied three well-characterized and closely related oral species, Streptococcus gordonii, Streptococcus sanguinis, and cariogenic Streptococcus mutans. S. sanguinis and S. gordonii used oxygen availability and the differential production of hydrogen peroxide (H2O2) to compete effectively against S. mutans. Interspecies antagonism was influenced by glucose with reduced production of H2O2. Furthermore, aerobic conditions stimulated the competence system and the expression of the bacteriocin mutacin IV of S. mutans, as well as the H2O2-dependent release of heterologous DNA from mixed cultures of S. sanguinis and S. gordonii. These data provide new insights into ecological factors that determine the outcome of competition between pioneer colonizing oral streptococci and the survival mechanisms of S. mutans in the oral biofilm.

scholarly journals Comparison of genes required for H2O2 resistance in Streptococcus gordonii and Streptococcus sanguinis

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2627-2638 ◽  
Author(s):  
Yifan Xu ◽  
Andreas Itzek ◽  
Jens Kreth
Keyword(s):  
Growth Conditions ◽  
Streptococcus Gordonii ◽  
Aerobic Growth ◽  
Anaerobic Conditions ◽  
Oral Biofilm ◽  
Streptococcus Sanguinis ◽  
Functional Studies ◽  
Stress Protection ◽  
H2o2 Resistance

Hydrogen peroxide (H2O2) is produced by several members of the genus Streptococcus mainly through the pyruvate oxidase SpxB under aerobic growth conditions. The acute toxic nature of H2O2 raises the interesting question of how streptococci cope with intrinsically produced H2O2, which subsequently accumulates in the microenvironment and threatens the closely surrounding population. Here, we investigate the H2O2 susceptibility of oral Streptococcus gordonii and Streptococcus sanguinis and elucidate potential mechanisms of how they protect themselves from the deleterious effect of H2O2. Both organisms are considered primary colonizers and occupy the same intraoral niche making them potential targets for H2O2 produced by other species. We demonstrate that S. gordonii produces relatively more H2O2 and has a greater ability for resistance to H2O2 stress. Functional studies show that, unlike in Streptococcus pneumoniae, H2O2 resistance is not dependent on a functional SpxB and confirms the important role of the ferritin-like DNA-binding protein Dps. However, the observed increased H2O2 resistance of S. gordonii over S. sanguinis is likely to be caused by an oxidative stress protection machinery present even under anaerobic conditions, while S. sanguinis requires a longer period of time for adaptation. The ability to produce more H2O2 and be more resistant to H2O2 might aid S. gordonii in the competitive oral biofilm environment, since it is lower in abundance yet manages to survive quite efficiently in the oral biofilm.

scholarly journals Similar genomic patterns of clinical infective endocarditis and oral isolates of Streptococcus sanguinis and Streptococcus gordonii

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Katrine Højholt Iversen ◽  
Louise Hesselbjerg Rasmussen ◽  
Kosai Al-Nakeeb ◽  
Jose Juan Almagro Armenteros ◽  
Christian Salgård Jensen ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Streptococcus Gordonii ◽  
Streptococcus Sanguinis ◽  
Genomic Patterns

scholarly journals Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

2009 ◽  
Vol 191 (20) ◽  
pp. 6281-6291 ◽  
Author(s):  
Jens Kreth ◽  
Hung Vu ◽  
Yongshu Zhang ◽  
Mark C. Herzberg
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Adhesion ◽  
Extracellular Dna ◽  
Release Mechanism ◽  
Streptococcus Gordonii ◽  
Oral Biofilm ◽  
Streptococcus Sanguinis ◽  
Production Of Hydrogen ◽  
Dna Release ◽  
Biofilm Development

ABSTRACT Extracellular DNA (eDNA) is produced by several bacterial species and appears to contribute to biofilm development and cell-cell adhesion. We present data showing that the oral commensals Streptococcus sanguinis and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H2O2). Surprisingly, S. sanguinis and S. gordonii cell integrity appears unaffected by conditions that cause autolysis in other eDNA-producing bacteria. Exogenous H2O2 causes release of DNA from S. sanguinis and S. gordonii but does not result in obvious lysis of cells. Under DNA-releasing conditions, cell walls appear functionally intact and ribosomes are retained over time. During DNA release, intracellular RNA and ATP are not coreleased. Hence, the release mechanism appears to be highly specific for DNA. Release of DNA without detectable autolysis is suggested to be an adaptation to the competitive oral biofilm environment, where autolysis could create open spaces for competitors to invade. Since eDNA promotes cell-to-cell adhesion, release appears to support oral biofilm formation and facilitates exchange of genetic material among competent strains.

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