Determination of in vivo immunomodulatory activity of mixed herbs extracts using total protein serum and depiction of lymphatic organ

2019 ◽  
Author(s):  
Dewi Tristantini ◽  
Adeline Jessica
Keyword(s):  
Total Protein ◽  
Immunomodulatory Activity ◽  
Lymphatic Organ ◽  
Protein Serum

Hemoglobin interference from in vivo hemolysis.

1985 ◽  
Vol 31 (9) ◽  
pp. 1566-1569 ◽  
Author(s):  
D W Blank ◽  
M H Kroll ◽  
M E Ruddel ◽  
R J Elin
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Intravascular Hemolysis ◽  
Serum Samples ◽  
Lactate Dehydrogenase Activity ◽  
Specimen Collection ◽  
Potassium Lactate

Abstract Laboratory values for specimens from a case of intravascular hemolysis showed that hemoglobin was significantly increased and thus could interfere with the determination of other analytes. We studied this problem by adding increasing amounts of purified hemoglobin (to a maximum concentration of 19.3 mg/L) to aliquots of pooled serum samples. The hemoglobin significantly interfered with the determination of only five analytes: albumin, aspartate aminotransferase, direct bilirubin, and total protein on the SMAC, and creatinine on the Astra. We propose that for cases of proven intravascular hemolysis, values for only the analytes not affected by hemoglobin should be reported. We find lactate dehydrogenase activity useful in assessing the components of in vivo hemolysis; the differences between serum and plasma values for potassium, lactate dehydrogenase, and hemoglobin are related to in vitro hemolysis. Criteria for specimen collection and assessment of type of hemolysis are proposed.

scholarly journals Immunomodulatory Activity Test of Syrup Dosage Form of Combination Phyllantus niruri Linn. And Sterculia quadrifida R.Br. Extract

2020 ◽  
Vol 11 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Rollando Rollando ◽  
Mauren Engracia ◽  
Eva Monica ◽  
Siswadi Siswadi
Keyword(s):  
Bark Extract ◽  
Immunomodulatory Activity ◽  
Macrophage Activity ◽  
Lattice Design ◽  
Activity Test ◽  
Tnf Α ◽  
Simplex Lattice

Meniran (Phyllantus niruri Linn.) is used generally as an immunomodulator and hepatoprotector. Empirically, bark of faloak (Sterculiaqudrifida R.Br.) is used as a medicinal plant to cure diseases such as hepatitis, gastroentritis, and diabetes. The combination of meniran extract and bark of faloak extract has the potential as a natural immunomodulatory agents. Immunomodulators are compositions of either recombinant, synthetic, or natural, which can restore the immune system. Therefore it is necessary to optimize the optimal formula in obtaining syrup preparations which have optimal immunomodulatory activity. The aim of this study was to find out the optimal syrup combination of meniran extract and faloak bark extract to produce optimal response values ​​in the in vitro immunomodulatory activity test with TNF-α and NF-κB parameters, and immunomodulatory activity tests in vivo with activity parameters macrophages. The extract was obtained through reflux extraction. The results of the extract were formulated and approved to determine their ability to induce TNF-α and NF-κB, and in the test of mice balb/b (Mus musculus L.) orally for 21 days. The lymph organs were isolated, rinsed with an RPMI-1640 solution, and analyzed using ELISA reader 450 nm. The determination of optimal formulas is done by using Rstudio v.3.5.3 software with ANOVA test parametric test and using Design Expert v.11 software using the Simplex Lattice Design (SLD) method. The results showed that the formula for the preparation of combination syrup to produce results induced TNF-α and NF-κB, and macrophage activity, namely the formula using meniran and faloak 0.96993: 0.0300704.

A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

1987 ◽  
Vol 26 (01) ◽  
pp. 1-6 ◽  
Author(s):  
S. Selvaraj ◽  
M. R. Suresh ◽  
G. McLean ◽  
D. Willans ◽  
C. Turner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tumor Cell ◽  
T Antigen ◽  
Human Carcinomas ◽  
Animal Tumor ◽  
Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

Determination of total protein in human blood by laser

2017 ◽  
Vol 13 (3) ◽  
pp. 261-274
Author(s):  
Sahra Salem Jassem ◽  
Keyword(s):  
Human Blood ◽  
Total Protein

Development and Pharmacokinetics Study of Antifungal Peptide Nanoliposomes by Liquid Chromatography-tandem Mass Spectrometry

2019 ◽  
Vol 15 (4) ◽  
pp. 312-318
Author(s):  
Shuoye Yang
Keyword(s):  
Mass Spectrometry ◽  
Biological Properties ◽  
Pharmacokinetic Property ◽  
Antifungal Peptide ◽  
Simple Liquid ◽  
Physico Chemical ◽  
Liquid Chromatography Tandem Mass ◽  
Pegylated Lipids

Background: The therapeutic ability and application of antifungal peptide (APs) are limited by their physico-chemical and biological properties, the nano-liposomal encapsulation would improve the in vivo circulation and stability. </P><P> Objective: To develop a long-circulating liposomal delivery systems encapsulated APs-CGA-N12 with PEGylated lipids and cholesterol, and investigated through in vivo pharmacokinetics. Methods: The liposomes were prepared and characterized, a rapid and simple liquid chromatographytandem mass spectrometry (LC-MS/MS) assay was developed for the determination of antifungal peptide in vivo, the pharmacokinetic characteristics of APs liposomes were evaluated in rats. Results: Liposomes had a large, unilamellar structure, particle size and Zeta potential ranged from 160 to 185 nm and -0.55 to 1.1 mV, respectively. The results indicated that the plasma concentration of peptides in reference solutions rapidly declined after intravenous administration, whereas the liposomeencapsulated ones showed slower elimination. The AUC(0-∞) was increased by 3.0-fold in liposomes in comparison with standard solution (20 mg·kg-1), the half-life (T1/2) was 1.6- and 1.5-fold higher compared to the reference groups of 20 and 40 mg·kg-1, respectively. Conclusion: Therefore, it could be concluded that liposomal encapsulation effectively improved the bioavailability and pharmacokinetic property of antifungal peptides.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

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