A study on chitosan macroparticles as potential affinity beads

2019 ◽  
Author(s):  
Fatin Fazrina Roslan ◽  
Fatin Nur Amirah Mohd Sabri ◽  
Noor Hidayah Che Lah ◽  
Nur Nabilah Shahidan ◽  
Muhammad Ashraf Shahidan
Keyword(s):  
Affinity Beads

scholarly journals Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae

2020 ◽  
Vol 375 (1801) ◽  
pp. 20190488 ◽  
Author(s):  
Takayuki Shimizu ◽  
Rintaro Yasuda ◽  
Yui Mukai ◽  
Ryo Tanoue ◽  
Tomohiro Shimada ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Proteomic Analysis ◽  
High Performance ◽  
Biochemical Analysis ◽  
Cyanidioschyzon Merolae ◽  
Genes Expression ◽  
Retrograde Signalling ◽  
Coordinated Expression ◽  
Retrograde Signal ◽  
Affinity Beads

Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae . Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

scholarly journals Production of magnetic GFP affinity beads using a Camelid anti-GFP nanobody v1 (protocols.io.3qqgmvw)

protocols.io ◽  
2019 ◽  
Author(s):  
Nichanok Auevechanichkul ◽  
Ruth Lintermann ◽  
Lennart Wirthmueller
Keyword(s):  
Affinity Beads

Spectral characterization of lysozyme adsorption on dye-affinity beads

2008 ◽  
Vol 108 (6) ◽  
pp. 3454-3461 ◽  
Author(s):  
Nilgün Basar ◽  
Lokman Uzun ◽  
Ali Güner ◽  
Adil Denizli
Keyword(s):  
Spectral Characterization ◽  
Lysozyme Adsorption ◽  
Affinity Beads

Target Protein Identification on Photocatalyst‐Functionalized Magnetic Affinity Beads

2020 ◽  
Vol 101 (1) ◽  
Author(s):  
Michihiko Tsushima ◽  
Shinichi Sato ◽  
Keita Nakane ◽  
Hiroyuki Nakamura
Keyword(s):  
Protein Identification ◽  
Target Protein ◽  
Affinity Beads

Lysozyme purification with dye-affinity beads under magnetic field

2007 ◽  
Vol 41 (3) ◽  
pp. 234-242 ◽  
Author(s):  
Nilgün Başar ◽  
Lokman Uzun ◽  
Ali Güner ◽  
Adil Denizli
Keyword(s):  
Magnetic Field ◽  
Affinity Beads

scholarly journals FR225659-binding Proteins: Identification as Serine/Threonine Protein Phosphatase PP1 and PP2A Using High-performance Affinity Beads

2004 ◽  
Vol 57 (7) ◽  
pp. 456-461 ◽  
Author(s):  
HIDETAKA HATORI ◽  
TATSUYA ZENKOH ◽  
MOTOO KOBAYASHI ◽  
YOSHIHIRO OHTSU ◽  
NOBUHARU SHIGEMATSU ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
High Performance ◽  
Binding Proteins ◽  
Affinity Beads

scholarly journals Microfluidic Single-Cell Analysis with Affinity Beads

Small ◽  
2015 ◽  
Vol 11 (29) ◽  
pp. 3511-3511 ◽  
Author(s):  
Michael Werner ◽  
Raghavendra Palankar ◽  
Loïc Arm ◽  
Ruud Hovius ◽  
Horst Vogel
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Affinity Beads

Development and application of high-performance affinity beads: Toward chemical biology and drug discovery

2009 ◽  
Vol 9 (1) ◽  
pp. 66-85 ◽  
Author(s):  
Satoshi Sakamoto ◽  
Yasuaki Kabe ◽  
Mamoru Hatakeyama ◽  
Yuki Yamaguchi ◽  
Hiroshi Handa
Keyword(s):  
Drug Discovery ◽  
High Performance ◽  
Chemical Biology ◽  
Affinity Beads

Rapid contrast evaluation method based on affinity beads and backscattered electron imaging for the screening of electron stains

Microscopy ◽  
2015 ◽  
Vol 64 (5) ◽  
pp. 361-368
Author(s):  
Hiroki Kaku ◽  
Kanako Inoue ◽  
Yoshinori Muranaka ◽  
Pyoyun Park ◽  
Kenichi Ikeda
Keyword(s):  
Evaluation Method ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Affinity Beads
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