scholarly journals Screening factors influencing chitinase production by Trichoderma virens using two level factorial design

Author(s):  
Rachmawaty ◽  
Syamsiah ◽  
Irma Suryani Idris ◽  
Halifah Pagarra ◽  
Hartati ◽  
...  
2008 ◽  
Vol 150 (2) ◽  
pp. 193-204 ◽  
Author(s):  
Suraini Abd-Aziz ◽  
Christine Cheryl Fernandez ◽  
Madihah Md. Salleh ◽  
Rosli Md. Illias ◽  
Mohd. Ali Hassan

2019 ◽  
Vol 967 ◽  
pp. 132-142
Author(s):  
Rachmawaty ◽  
Pagarra Halifah ◽  
Hartati ◽  
Zulkifli Maulana ◽  
Madihah Md. Salleh

Physical factor for chitinase production by Trichodermavirens was first carried out using screening factor of 2-level factorial. The design was employed by selecting incubation time, temperature, moisture substrate, pH, inoculums size and concentration ammonium sulphate as a model factors. The result of 2-level factorial design experiment showed thal all three independent variable have significant effect on chitinase production. The physical factor was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of three variables including various incubation time, temperature and moisture substrate were created using Design Expert® Software, Version 6.0.4 The design consist of 20 experiments, which include 6 replicate at center points. The optimal value for each variable are incubation time sixth days, temperature 27.83°C and moisture substrate 54% with predicted chitinase activity of 0.48738 U/g of dry substrate. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.48864 U/g of dry substrate, which is similar to the predicted value. The obtained value of the chitinase production was 0.48738 U/g IDS, which was 1.2 fold higher than that of the 2-level factorial design (0.261 U/gds)


1977 ◽  
Vol 40 (1) ◽  
pp. 131-138 ◽  
Author(s):  
Sherman Eisenthal ◽  
Aaron Lazare

The expressions of requests of 120 patients who came to the Walk-In Psychiatric Clinic of a general hospital were examined to determine whether patients verbalized specific requests during the intake interview. As 63% of the patients verbalized a specific request, 37% emitted and 27% elicited ones, we concluded that many patients could have been more specific but felt restrained by their role. To evaluate the effect of the patients' attitudes and interviewers' behavior on the expression of requests, in a two-by-two factorial design, we varied the number of patients taking the Patient Request Form and/or being probed for a request during a standardized pre-intake interview. These two procedures did not affect significantly the number expressing requests: experimental groups 73% versus control group 63%. Completing the form elicited more requests. The interviewer variables of number and wording of probes for requests significantly facilitated expression of requests. In the discussion we attempted to evaluate factors influencing patient's resistance to the expression of requests and to place the meaning of requests in the context of more effective clinical functioning, particularly a negotiated approach to the treatment of patients.


Author(s):  
Sudhansu Sekhar Biswal ◽  
Chittaranjan Panda ◽  
Sudarsan Sahoo ◽  
Trilochan Jena ◽  
Kishor Chandra Panda

Strain ◽  
2007 ◽  
Vol 43 (4) ◽  
pp. 340-344 ◽  
Author(s):  
B. Ryan ◽  
E. G. Little ◽  
E. Murphy ◽  
J. Griffin

2019 ◽  
Vol 20 (2) ◽  
pp. 168-177
Author(s):  
Doddy Primayudia

ABSTRACTInformation is a crucial point in order to helps managers in decision making. Employees who acts as an agent on agency theory, will gladly report their achievement to their principal. On the other hand, employees tend to cover their faults, especially if the company’s targets are not achieved. There are many factors influencing the employees’ willingness to share the informations, prior to their faults or errors, and two of them are culture and the existence of the supervisor. This research uses experimental method and 2 × 2 factorial design to test the hypotheses. The sample in this research are low-acculturated Tionghoa (TAR) and high-acculturated Tionghoa (TAR). After the research is done, it is come to conclusion that; (1) employees’ cultural background and acculturation did affect their willingness in information sharing. (2) the presence of supervisor did not affect employees’ willingness in information sharing prior to their faults and errors.Keywords: Culture, Acculturation, Behavioral Accounting, Information Sharing


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