scholarly journals Cloning and molecular analysis of Candida albicans secreted aspartyl proteinase 4 (SAP4) Indonesian isolate

2018 ◽  
Author(s):  
Alfin Titian Permata ◽  
Afiono Agung Prasetyo ◽  
Sri Haryati
Keyword(s):  
Candida Albicans ◽  
Molecular Analysis ◽  
Secreted Aspartyl Proteinase

scholarly journals Germ Tubes and Proteinase Activity Contribute to Virulence of Candida albicans in Murine Peritonitis

1999 ◽  
Vol 67 (12) ◽  
pp. 6637-6642 ◽  
Author(s):  
Marianne Kretschmar ◽  
Bernhard Hube ◽  
Thomas Bertsch ◽  
Dominique Sanglard ◽  
Renate Merker ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Bowel Perforation ◽  
Tube Length ◽  
Triple Mutant ◽  
Liver And Pancreas ◽  
Organ Specific ◽  
Secreted Aspartyl Proteinase ◽  
Pepstatin A

ABSTRACT Peritonitis with Candida albicans is an important complication of bowel perforation and continuous ambulatory peritoneal dialysis. To define potential virulence factors, we investigated 50 strains of C. albicans in a murine peritonitis model. There was considerable variation in their virulence in this model when virulence was measured as release of organ-specific enzymes into the plasma of infected mice. Alanine aminotransferase (ALT) and α-amylase (AM) were used as parameters for damage of the liver and pancreas, respectively. The activities of ALT and AM in the plasma correlated with invasion into the organs measured in histologic sections and the median germ tube length induced with serum in vitro. When the activity of proteinases was inhibited in vivo with pepstatin A, there was a significant reduction of ALT and AM activities. This indicates that proteinases contributed to virulence in this model. Using strains ofC. albicans with disruption of secreted aspartyl proteinase gene SAP1, SAP2, SAP3, orSAP4 through SAP6 (collectively referred to asSAP4-6), we showed that only a Δsap4-6 triple mutant induced a significantly reduced activity of ALT in comparison to the reference strain. In contrast to the Δsap1, Δsap2, and Δsap3 mutants, the ALT induced by the Δsap4-6 mutant could not be further reduced by pepstatin A treatment, which indicates that Sap4-6 may contribute to virulence in this model.

scholarly journals Secreted Aspartyl Proteinases and Interactions of Candida albicans with Human Endothelial Cells

1998 ◽  
Vol 66 (6) ◽  
pp. 3003-3005 ◽  
Author(s):  
Ashraf S. Ibrahim ◽  
Scott G. Filler ◽  
Dominique Sanglard ◽  
John E. Edwards ◽  
Bernhard Hube
Keyword(s):  
Endothelial Cells ◽  
Candida Albicans ◽  
Endothelial Cell ◽  
Cell Interactions ◽  
Human Endothelial Cells ◽  
Secreted Aspartyl Proteinase ◽  
Null Mutants ◽  
Aspartyl Proteinases

ABSTRACT The endothelial cell interactions of homozygous null mutants ofCandida albicans that were deficient in secreted aspartyl proteinase 1 (Sap1), Sap2, or Sap3 were investigated. Only Sap2 was found to contribute to the ability of C. albicans to damage endothelial cells and stimulate them to express E-selectin. None of the Saps studied appears to play a role in C. albicansadherence to endothelial cells.

Molecular Analysis of Candida albicans Isolates from Clinical Specimens

2009 ◽  
Vol 169 (4) ◽  
pp. 261-267 ◽  
Author(s):  
Melahat Gurbuz ◽  
Ilknur Kaleli
Keyword(s):  
Candida Albicans ◽  
Molecular Analysis ◽  
Clinical Specimens
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