scholarly journals Phylogenetic analysis based on internal transcribed spacer region (ITS1-5.8S-ITS2) of Aspergillus niger producing phytase from Indonesia

2018 ◽  
Author(s):  
Baraka Stewart Mkumbe ◽  
Sajidan ◽  
Artini Pangastuti ◽  
Ari Susilowati
Keyword(s):  
Phylogenetic Analysis ◽  
Aspergillus Niger ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region

scholarly journals A new concept for Dictyostelium sphaerocephalum based on morphology and phylogenetic analysis of nuclear ribosomal internal transcribed spacer region sequences

2007 ◽  
Vol 85 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Maria Romeralo ◽  
Omar Fiz-Palacios ◽  
Carlos Lado ◽  
James C. Cavender
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Morphological Plasticity ◽  
Branching Pattern ◽  
Classical Concept ◽  
Internal Transcribed Spacer Region

Three dictyostelid isolates were found in Spain and Argentina that are morphologically different from known species. These isolates have some features similar to Dictyostelium sphaerocephalum (Oudem.) Sacc., Marchal & É.J. Marchal, but differ in size and sorocarp branching pattern. We sequenced the nuclear ribosomal internal transcribed spacer region to explore phylogenetic relationships among this group of species, including the three new isolates and their closest relatives. In all phylogenetic analyses performed, sequences of all three isolates group together with sequences from “typical” D. sphaerocephalum samples. This result supports previous observations of the morphological plasticity in dictyostelids, especially D. sphaerocephalum, leading us to broaden the classical concept of this species.

Comparative studies of fungi associated with sea foods in different wetlands of Akwa Ibom State

2020 ◽  
Vol 34 (1) ◽  
pp. 41-47
Author(s):  
I.N. Bassey ◽  
N.U. Asamudo
Keyword(s):  
Phylogenetic Analysis ◽  
Food Security ◽  
Aspergillus Niger ◽  
Internal Transcribed Spacer ◽  
Natural Populations ◽  
Pcr Amplification ◽  
Potato Dextrose Agar ◽  
Molecular Technique ◽  
Fungal Isolates ◽  
Fish Samples

The study investigated fungi associated with sea foods, fish (Pellonula leonensis) and crayfish (Parapandalus pritis) from different wetlands (Ibeno, Ikot Abasi, Eket and Itu) in Akwa Ibom State. Samples were collected and cultured on Potato Dextrose Agar (PDA) and the isolated fungi were identified using molecular technique. Based on PCR amplification, sequencing of the internal transcribed spacer and phylogenetic analysis, the fungi identified were: Aspergillus niger, A. felis, A. foetidus, A. aculeatus, A. japonicus, A. flavus, A. tamari, A. terreus, Penicilium citrinum, Candida tropicalis and Trametes polyzona. The most commonly isolated fungi from fish for the four locations were A. niger (52.8) in Itu, P. citrinum (51.7) in Ibeno, A. foetidus (53.5) in Ikot Abasi and A. aculeatus (51.6) in Eket. For samples of crayfish, the most commonly isolated fungi were A. niger (25.1) in Itu and A. foetidus (20.1) in Ikot Abasi. Generally, Ibeno recorded the highest frequencies of fungal isolates while Ikot Abasi had the least. Percentage occurrence of fungi isolated from fish samples were significantly higher (p<0.05) when compared with those isolated from crayfish samples. The presence of these fungi showed that sea foods were exposed to an increasing number of virulent infectious diseases in natural populations, and fungal-like diseases can cause some of the most severe die-offs and extinctions ever witnessed in wild species thus jeopardizing food security Keywords: Fungi, Sea food, Wetland, Aspergillus, Fish.

Inocybe griseorubida, a new species of Pseudosperma clade from tropical India

Phytotaxa ◽  
2015 ◽  
Vol 221 (2) ◽  
pp. 166 ◽  
Author(s):  
K. P. Deepna Latha ◽  
Patinjareveettil Manimohan
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rna Polymerase Ii ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Similar Species ◽  
Internal Transcribed Spacer Region ◽  
Comprehensive Description ◽  
Kerala State ◽  
A New Species

Inocybe griseorubida sp. nov. is described from Kerala State, India. A comprehensive description, photographs, and comparisons with phenetically similar species are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (rpb2) gene of this species were sequenced and analyzed. Phylogenetic analysis of rpb2 sequences confirmed both the novelty of the species and its placement within the Pseudosperma clade.

New and noteworthy species of white Entoloma (Agaricales, Entolomataceae) in China

Phytotaxa ◽  
2015 ◽  
Vol 205 (2) ◽  
pp. 99 ◽  
Author(s):  
Xiao-Lan He ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
Bing-Cheng Gan
Keyword(s):  
Phylogenetic Analysis ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Monophyletic Group ◽  
Southwestern China ◽  
High Altitudes ◽  
Internal Transcribed Spacer Region ◽  
The Common

Three species of Entoloma with white basidioma in subgenus Alboleptonia from China are reported in this paper. Entoloma crocotillum is described as new to science from high altitudes in southwestern China. It is characterized by the small, white to pinkish pileus covered with matted-appressed fibrils, relatively large basidiospores with 5–6 angles, and the common presence of clamp-connections. Entoloma sulcatum, a new Chinese record, and E. stylophorum are documented based on new collections. Additionally, ITS (internal transcribed spacer region), nLSU (nuclear large subunit), and RPB2 (RNA polymerase II second largest subunit) sequences of E. crocotillum were successfully generated, and the phylogenetic positions of the three species among Alboleptonia species and other entolomatoid groups are preliminarily analyzed based on the combined nLSU and RPB2 dataset. The phylogenetic analysis showed that E. crocotillum is most closely related to E. sericellum and that Alboleptonia is not a monophyletic group.

Reclassification of Graphium tectonae as Parascedosporium tectonae gen. nov., comb. nov., Pseudallescheria africana as Petriellopsis africana gen. nov., comb. nov. and Pseudallescheria fimeti as Lophotrichus fimeti comb. nov.

2007 ◽  
Vol 57 (9) ◽  
pp. 2171-2178 ◽  
Author(s):  
Fèlix Gilgado ◽  
Josepa Gené ◽  
Josep Cano ◽  
Josep Guarro
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Soil Fungi ◽  
New Genus ◽  
New Combination ◽  
Internal Transcribed Spacer Region ◽  
Biodiversity Survey ◽  
Morphological Studies ◽  
5.8S Rrna ◽  
Type Strains

During a biodiversity survey of Argentinian soil fungi, we recovered a rare Scedosporium-like fungus which was proven to be genetically and morphologically different from known species of Scedosporium (anamorph of Pseudallescheria) and relatives and is proposed here as representing a new genus. This genus is mainly characterized by producing sympodial conidia from denticulate conidiogenous cells. This isolate was morphologically identical to Graphium tectonae and thus the new combination Parascedosporium tectonae gen. nov., comb. nov. is proposed. Sequence analysis of four regions of three genes, i.e. β-tubulin (two loci), calmodulin and the internal transcribed spacer region of the 5.8S rRNA, confirmed our proposal. Both the phylogenetic analysis and morphological studies excluded Pseudallescheria africana and Pseudallescheria fimeti from the genus Pseudallescheria. The former is proposed as a member of the new genus Petriellopsis, and the latter has been accommodated in Lophotrichus. The type strains of Parascedosporium tectonae gen. nov., comb. nov., Petriellopsis africana gen. nov., comb. nov. and Lophotrichus fimeti comb. nov. are respectively CBS 127.84T, CBS 311.72T and CBS 129.78T.

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