Plant leaves mediated synthesis of semiconductor ZnO nanoparticles and its application for seed germination

Author(s):  
Manpreet Singh ◽  
Jagpreet Singh ◽  
Deepanjali Sharma ◽  
Bhupinder Kaur ◽  
Mohit Rawat
Author(s):  
H. L. Nedved ◽  
J. N. Kalatskaja ◽  
N. A. Laman ◽  
V. V. Minkova ◽  
K. M. Herasimovich ◽  
...  

It was established that the treatment of clover seeds with radio frequency (RF) electromagnetic field and low pressure (200 Pa) RF plasma excited at a frequency of 5.28 MHz has a stimulating effect both on the germination of seeds and on the growth and development of plants grown in laboratory and field conditions. Plasma treatment for 5 min led to the greatest stimulation of seed germination and germination energy, a significant increase in the biomass of shoots and roots. At the same time, the content of phenolic compounds and flavonoids in plant leaves decreased.


Nanoparticles have achieved novel applications in biotechnology and agricultural industries. Nanoparticles on plants may cover a new insight to the ecosystems. There is a need for advance study of the possible effects of the nanoparticles on plant growth and development. In the present investigation, zinc oxide nanoparticles have been prepared by the simple chemical route and were authorized by UV-vis spectrophotometer and X-ray diffraction (XRD) analysis alongwith transmission electron microscope (TEM). The consequence of various concentrations of synthesized zinc oxide nanoparticles on wheat seeds (variety: lok-1) was studied by soaking approach and follow its effect on seedling growth of wheat (at 5 days). The seed germination, plant growth & chlorophyll content characteristics were measured by using standard biophysical techniques and studied. Results showed enhancement in germination and growth characteristics in five days grown wheat seedlings for control upto thousand ppm. Above thousand ppm, the considerable drop off was observed in these parameters upto two thousand ppm. Also, the chlorophyll content in the control sample is greater than the samples treated with the various concentrations of zinc oxide nanoparticles. There was a noticeable effect that employing suitable concentration of ZnO nanoparticles could support the seed germination of wheat in contrast to untreated control.


2020 ◽  
Vol 15 (11) ◽  
pp. 1412-1422
Author(s):  
Nishat Arshi ◽  
Y. Prashanthi ◽  
Tentu Nageswara Rao ◽  
Faheem Ahmed ◽  
Shalendra Kumar ◽  
...  

In this study, we report synthesis of Zinc oxide nanoparticles using simple chemical and green methods. The ZnO nanoparticles were synthesized using leaf extract of Azadirachta indica (neem) as reducing agent. The as obtained product was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray analysis (EDAX) and TEM techniques. XRD analysis confirms that ZnO nanoparticles were crystalline having hexagonal Wurtzite structure with (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0) and (1 1 2) planes. SEM analyses show that the as synthesized ZnO NPs were in the form of agglomerates and no other impurity peak was found in the EDS. TEM analyses confirm that the size of the nanoparticle was approx. 50 nm. Here in, we investigate the effect of chemical and green synthesized zinc oxide nanoparticles on germination and growth of lycopersicum esculentus (tomato) using petri plate seed germination method in loamy sand soil. The impact of concentration of applied ZnO nanoparticles via green synthesis and chemical methods were analyzed. Results revealed that green synthesized Zinc oxide nanoparticles showed maximum growth of seedling as compared to chemically synthesized Zinc oxide nanoparticles, bulk ZnO and control. After 50 days of tomato growth analysis, it was recognized that ZnO NPs can be a good green synthetic fertilizer by increasing shoot length, wet weight, dry weight and yield over conventional control. Hence, green method is found to be more effective.


Nanoparticles have achieved novel applications in biotechnology and agricultural industries. Nanoparticles on plants may cover a new insight to the ecosystems. There is a need for advance study of the possible effects of the nanoparticles on plant growth and development. In the present investigation, zinc oxide nanoparticles have been prepared by the simple chemical route and were authorized by UV-vis spectrophotometer and X-ray diffraction (XRD) analysis alongwith transmission electron microscope (TEM). The consequence of various concentrations of synthesized zinc oxide nanoparticles on wheat seeds (variety: lok-1) was studied by soaking approach and follow its effect on seedling growth of wheat (at 5 days). The seed germination, plant growth & chlorophyll content characteristics were measured by using standard biophysical techniques and studied. Results showed enhancement in germination and growth characteristics in five days grown wheat seedlings for control upto thousand ppm. Above thousand ppm, the considerable drop off was observed in these parameters upto two thousand ppm. Also, the chlorophyll content in the control sample is greater than the samples treated with the various concentrations of zinc oxide nanoparticles. There was a noticeable effect that employing suitable concentration of ZnO nanoparticles could support the seed germination of wheat in contrast to untreated control.


2020 ◽  
Vol 25 ◽  
pp. 224-229
Author(s):  
Jaiby Cyriac ◽  
Krishnakumar Melethil ◽  
Bejoy Thomas ◽  
M. Sreejit ◽  
Thomas Varghese

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