scholarly journals Characterization of cycloartenol synthase KcCAS promoter region from mangrove plant (Kandelia obovata Sheue, H. Y. Liu & J. Yong)

2018 ◽  
Author(s):  
Mohammad Basyuni ◽  
Ridha Wati ◽  
Shigeyuki Baba ◽  
Hirosuke Oku
Keyword(s):  
Promoter Region ◽  
Kandelia Obovata ◽  
Mangrove Plant ◽  
Cycloartenol Synthase

scholarly journals Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jiao Fei ◽  
You-shao Wang ◽  
Hao Cheng ◽  
Yu-bin Su ◽  
Yongjia Zhong ◽  
...  
Keyword(s):  
Cold Resistance ◽  
Three Dimensional ◽  
Kandelia Obovata ◽  
Mangrove Plant ◽  
Calculated Molecular Mass ◽  
Pathogenesis Related ◽  
Localization Analysis ◽  
Response To Stress ◽  
Sequence Encoding

Abstract Background Low temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported. Results In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were common signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) model of KoOsmotin, contained one α-helix and eleven β-strands, was formed by three characteristic domains. Database comparisons of the KoOsmotin showed the closest identity (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree also revealed that the KoOsmotin was clustered in the branch of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to both the plasma membrane and cytoplasm by the subcellular localization analysis. Gene expression showed that the KoOsmotin was induced primarily and highly in the leaves of K. obovata, but less abundantly in stems and roots. The overexpressing of KoOsmotin conferred cold tolerance in Escherichia coli cells. Conclusion As we known, this is the first study to explore the osmotin of K. obovata. Our study provided valuable clues for further exploring the function of KoOsmotin response to stress.

scholarly journals Characterization of the human pleiotrophin gene. Promoter region and chromosomal localization.

1992 ◽  
Vol 267 (36) ◽  
pp. 26011-26016 ◽  
Author(s):  
Y.S. Li ◽  
R.M. Hoffman ◽  
M.M. Le Beau ◽  
R Espinosa ◽  
N.A. Jenkins ◽  
...  
Keyword(s):  
Promoter Region ◽  
Chromosomal Localization ◽  
Gene Promoter ◽  
Gene Promoter Region

scholarly journals Characterization of the promoter region of the mouse Xist gene.

1995 ◽  
Vol 92 (26) ◽  
pp. 12515-12519 ◽  
Author(s):  
N. Pillet ◽  
C. Bonny ◽  
D. F. Schorderet
Keyword(s):  
Promoter Region ◽  
Xist Gene

scholarly journals Characterization of Hepatic-specific Regulatory Elements in the Promoter Region of the Human Cholesterol 7α-Hydroxylase Gene

1997 ◽  
Vol 272 (6) ◽  
pp. 3444-3452 ◽  
Author(s):  
Allen D. Cooper ◽  
Jean Chen ◽  
Mary Jane Botelho-Yetkinler ◽  
Yicheng Cao ◽  
Takahiro Taniguchi ◽  
...  
Keyword(s):  
Promoter Region ◽  
Regulatory Elements ◽  
Hydroxylase Gene

scholarly journals The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas
Keyword(s):  
Amino Acids ◽  
Agrobacterium Tumefaciens ◽  
Molecular Mass ◽  
Promoter Region ◽  
Consensus Sequence ◽  
Tumor Formation ◽  
Nicotiana Glauca ◽  
Agrobacterium Vitis ◽  
Ti Plasmids

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress

Ecotoxicology ◽  
2021 ◽  
Author(s):  
Jiao Fei ◽  
You-Shao Wang ◽  
Hao Cheng ◽  
Fu-Lin Sun ◽  
Cui-Ci Sun
Keyword(s):  
Cold Stress ◽  
Kandelia Obovata ◽  
Mangrove Plant ◽  
Proteomic Analyses
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