scholarly journals Microfluidic implementation of functional cytometric microbeads for improved multiplexed cytokine quantification

2018 ◽  
Vol 12 (4) ◽  
pp. 044112 ◽  
Author(s):  
Ya Liu ◽  
Jiyu Li ◽  
Dinglong Hu ◽  
Josh H. M. Lam ◽  
Dong Sun ◽  
...  
Keyword(s):  
Cytokine Quantification

scholarly journals Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Joleen Lopes Machado ◽  
Anne Karine Martins Assunção ◽  
Mayara Cristina Pinto da Silva ◽  
Aramys Silva dos Reis ◽  
Graciomar Conceição Costa ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Pulmonary Inflammation ◽  
Oral Route ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Water Control ◽  
Cotton Pellet Granuloma ◽  
Anti Inflammatory ◽  
Acute And Chronic Inflammation ◽  
Cytokine Quantification

The immunomodulatory and anti-inflammatory activities of green propolis extracts fromApis melliferawere investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) ofEscherichia coliby intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.

scholarly journals Cardiorenal Syndrome Type 1: A Defective Regulation of Monocyte Apoptosis Induced by Proinflammatory and Proapoptotic Factors

2015 ◽  
Vol 5 (2) ◽  
pp. 105-115 ◽  
Author(s):  
Silvia Pastori ◽  
Grazia Maria Virzì ◽  
Alessandra Brocca ◽  
Massimo de Cal ◽  
Anna Clementi ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Cardiorenal Syndrome ◽  
Syndrome Type ◽  
Caspase Activity ◽  
Similar Concentration ◽  
Immune Mediated ◽  
Tnf Α ◽  
Monocyte Apoptosis ◽  
Cytokine Quantification

In this study, we examined the possible immune-mediated mechanisms in cardiorenal syndrome (CRS) type 1 pathogenesis. We enrolled 40 patients with acute heart failure (AHF), 11 patients with CRS type 1 and 15 controls. Plasma from the different groups was incubated with monocytes; subsequently, cell apoptosis was evaluated by DNA fragmentation, caspase activity and cytofluorometric assay. Cytokine quantification in plasma and supernatant was performed by ELISA. Monocytes treated with CRS type 1 plasma showed significantly higher apoptosis compared with those treated with AHF and the controls (p < 0.05). Caspase-3 (CRS type 1: 2.20 ng/ml, IQR 2.06-2.33; AHF: 1.48 ng/ml, IQR 1.31-1.56; controls: 0.71 ng/ml, IQR 0.67-0.81) and caspase-8 levels (CRS type 1: 1.49 ng/ml, IQR 1.42-1.57; AHF: 0.94 ng/ml, IQR 0.84-0.98; controls: 0.56 ng/ml, IQR 0.51-0.58) in cells incubated with plasma from these patients demonstrated a significantly higher concentration. We observed a strong upregulation of plasma IL-6 and IL-18 in CRS type 1 compared with AHF and the controls (p < 0.05). Interestingly, we observed a similar concentration of TNF-α in CRS type 1 and AHF. In CRS type 1 patients, IL-6 (52.13 ng/ml, IQR 47.29-66.83) and IL-18 levels (197.75 ng/ml, IQR 120.80-265.49) in supernatant were significantly higher than in AHF patients (IL-6: 28.79 ng/ml, IQR 19.90-36.10; IL-18: 21.98 ng/ml, IQR 15.98-29.85) and controls (IL-6: 5.02 ng/ml, IQR 4.56-6.44; IL-18: 7.91 ng/ml, IQR 5.57-10.62). These findings suggest the presence of a defective regulation of monocyte apoptosis in CRS type 1 patients and the involvement of an immune-mediated mechanism in the pathophysiology of this syndrome.

Cytokine quantification in abomasal lymph node of bovine infected with Haemonchus spp. parasites

2009 ◽  
Vol 128 (1-3) ◽  
pp. 289
Author(s):  
Adriana M.G. Ibelli ◽  
Liliane C. Nakata ◽  
Rogério Andréo ◽  
Márcia C.S. Oliveira ◽  
Luiz L. Coutinho ◽  
...  
Keyword(s):  
Lymph Node ◽  
Cytokine Quantification

scholarly journals Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

2021 ◽  
Vol 22 (14) ◽  
pp. 7496
Author(s):  
Isabel Olmos Calvo ◽  
Olga Kuten-Pella ◽  
Karina Kramer ◽  
Ágnes Madár ◽  
Szilvia Takács ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Platelet Rich Plasma ◽  
Cell Types ◽  
Inflammatory Conditions ◽  
Osteoarthritic Chondrocytes ◽  
Prp Gene ◽  
Human Explants ◽  
Cytokine Quantification

Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher Col1a1 expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration.

Intraocular cytokine quantification of experimental autoimmune uveoretinitis in rats

1998 ◽  
Vol 6 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Annabelle A. Okada ◽  
Jun-ichi Sakai ◽  
Masahiko Usui ◽  
J. Junichiro Mizuguchi
Keyword(s):  
Experimental Autoimmune Uveoretinitis ◽  
Cytokine Quantification ◽  
Experimental Autoimmune

scholarly journals Assessment of the acid phosphatase CP01850 from Corynebacterium pseudotuberculosis in DNA and subunit vaccine formulations against caseous lymphadenitis

2020 ◽  
Vol 72 (1) ◽  
pp. 199-207
Author(s):  
A.F.S. Rezende ◽  
A.A. Brum ◽  
F.S.B. Bezerra ◽  
D.C. Braite ◽  
G.L. Sá ◽  
...  
Keyword(s):  
Survival Rate ◽  
Dna Vaccine ◽  
Subunit Vaccine ◽  
Corynebacterium Pseudotuberculosis ◽  
Caseous Lymphadenitis ◽  
Lethal Challenge ◽  
Recombinant Subunit Vaccine ◽  
Th2 Immune Response ◽  
Ifn Γ ◽  
Cytokine Quantification

ABSTRACT The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice were divided in five groups containing eight animals each, inoculated with: pTARGET/cp01850 as DNA vaccine (G1); rCP01850 plus Al (OH)3 as recombinant subunit vaccine (G2); pTARGET/cp01850 and a boost with rCP01850 plus Al (OH)3 (G3); pTARGET (G4); or Al (OH)3 (G5). Mice were inoculated and blood samples were collected on days 0, 21, and 42 for the analysis of total IgG, IgG1 and IgG2a by ELISA. In each group, five animals were challenged with Mic-6 C. pseudotuberculosis strain, and three were used for cytokine quantification by qPCR. Although no group has been protected by vaccines against lethal challenge, G2 showed an increase in the survival rate after challenge. Significantly higher levels of IL-4, IL-12, IFN-γ, total IgG, IgG1 and IgG2a were also detected for G2, evidencing a mixed Th1/Th2 immunological profile. In conclusion, despite no protection level provided by different vaccinal strategies using cp1002_RS01850 from C. pseudotuberculosis, G2 developed a Th1/Th2 immune response with an increase in survival rate.

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