scholarly journals Integration of cell-free protein synthesis and purification in one microfluidic chip for on-demand production of recombinant protein

2018 ◽  
Vol 12 (5) ◽  
pp. 054102 ◽  
Author(s):  
Xiao Xiao ◽  
Yuan Zhou ◽  
Yuqiong Sun ◽  
Qing Wang ◽  
Jianbo Liu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Recombinant Protein ◽  
Microfluidic Chip ◽  
Free Protein ◽  
On Demand ◽  
Cell Free Protein Synthesis

scholarly journals Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials

2018 ◽  
Vol 7 (3) ◽  
pp. 875-884 ◽  
Author(s):  
Ana I. Benítez-Mateos ◽  
Irantzu Llarena ◽  
Ana Sánchez-Iglesias ◽  
Fernando López-Gallego
Keyword(s):  
Protein Synthesis ◽  
One Pot ◽  
Free Protein ◽  
On Demand ◽  
Cell Free Protein Synthesis

Endotoxin-Free E. coli- Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production

2018 ◽  
Vol 14 (3) ◽  
pp. 1800271 ◽  
Author(s):  
Kristen M. Wilding ◽  
John P. Hunt ◽  
Joshua W. Wilkerson ◽  
Parker J. Funk ◽  
Rebecca L. Swensen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
E Coli ◽  
On Demand ◽  
Cell Free Protein Synthesis

Flexible on-demand cell-free protein synthesis platform based on a tube-in-tube reactor

2020 ◽  
Vol 5 (2) ◽  
pp. 270-277 ◽  
Author(s):  
Caijin Zhou ◽  
Xiaomei Lin ◽  
Yuan Lu ◽  
Jisong Zhang
Keyword(s):  
Protein Synthesis ◽  
Protein Drugs ◽  
Free Protein ◽  
On Demand ◽  
Continuous Synthesis ◽  
Tube Reactor ◽  
Cell Free Protein Synthesis

A flexible on-demand cell-free protein synthesis platform using a tube-in-tube reactor is established for continuous synthesis of different protein drugs.

scholarly journals Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator

2019 ◽  
Vol 2 (2) ◽  
pp. 52 ◽  
Author(s):  
Seung-Ook Yang ◽  
Gregory H. Nielsen ◽  
Kristen M. Wilding ◽  
Merideth A. Cooper ◽  
David W. Wood ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Wholesale Price ◽  
Free System ◽  
Free Protein ◽  
E Coli ◽  
On Demand ◽  
Tissue Plasminogen ◽  
Cell Free Protein Synthesis

Stroke is the leading cause of death with over 5 million deaths worldwide each year. About 80% of strokes are ischemic strokes caused by blood clots. Tissue plasminogen activator (tPa) is the only FDA-approved drug to treat ischemic stroke with a wholesale price over $6000. tPa is now off patent although no biosimilar has been developed. The production of tPa is complicated by the 17 disulfide bonds that exist in correctly folded tPA. Here, we present an Escherichia coli-based cell-free protein synthesis platform for tPa expression and report conditions which resulted in the production of active tPa. While the activity is below that of commercially available tPa, this work demonstrates the potential of cell-free expression systems toward the production of future biosimilars. The E. coli-based cell-free system is increasingly becoming an attractive platform for low-cost biosimilar production due to recent developments which enable production from shelf-stable lyophilized reagents, the removal of endotoxins from the reagents to prevent the risk of endotoxic shock, and rapid on-demand production in hours.

Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free therapeutic production

2019 ◽  
Vol 53 ◽  
pp. 73-80 ◽  
Author(s):  
Kristen M. Wilding ◽  
Emily Long Zhao ◽  
Conner C. Earl ◽  
Bradley C. Bundy
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
On Demand ◽  
Cell Free Protein Synthesis

scholarly journals Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40588-40596
Author(s):  
Tony Köhler ◽  
Thomas Heida ◽  
Sandra Hoefgen ◽  
Niclas Weigel ◽  
Vito Valiante ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
In Situ Immobilization ◽  
Bottom Up ◽  
Free Protein ◽  
Malonyl Coa ◽  
Cell Free Protein Synthesis

We describe a bottom-up approach towards functional enzymes utilizing microgels as carriers for genetic information that enable cell-free protein synthesis, in situ immobilization, and utilization of functional deGFP-MatB.

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