Stable and selective self-assembly of α-lipoic acid on Ge(001) for biomolecule immobilization

2018 ◽  
Vol 123 (17) ◽  
pp. 175305 ◽  
Author(s):  
M. Kazmierczak ◽  
J. Flesch ◽  
J. Mitzloff ◽  
G. Capellini ◽  
W. M. Klesse ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Self Assembly ◽  
Biomolecule Immobilization

Recent advances in biomolecule immobilization based on self-assembly: organic–inorganic hybrid nanoflowers and metal–organic frameworks as novel substrates

2018 ◽  
Vol 6 (11) ◽  
pp. 1581-1594 ◽  
Author(s):  
Zhixian Lei ◽  
Chenling Gao ◽  
Lei Chen ◽  
Yanting He ◽  
Wende Ma ◽  
...  
Keyword(s):  
Self Assembly ◽  
Metal Organic Frameworks ◽  
Inorganic Hybrid ◽  
The Past ◽  
Biomolecule Immobilization ◽  
Recent Advances ◽  
Metal Organic

In the past few years, the immobilization of biomolecules on hybrid nanoflowers and metal–organic frameworks (MOFs) via self-assembly synthesis has received much attention due to its superior advantages.

Synthesis and self-assembly of a novel cobalt(II) porphyrin lipoic acid derivative on gold

2010 ◽  
Vol 14 (01) ◽  
pp. 101-107 ◽  
Author(s):  
Christoph S. Eberle ◽  
Ana S. Viana ◽  
Franz-Peter Montforts ◽  
Luisa Maria Abrantes
Keyword(s):  
Acid Derivative ◽  
Lipoic Acid ◽  
Self Assembly ◽  
Acidic Solution ◽  
Organic Medium ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Self Assembled Monolayer ◽  
Assembly Method ◽  
Blood Pigment

A novel Co(II) porphyrin lipoic acid derivative was synthesized starting from the commerically available red blood pigment hemin. The disulfide functionalities of the lipoic acid moieties allowed its immobilization on gold by a self-assembly method. The Co(II) porphyrin self-assembled monolayer (SAMs) on gold (111) surfaces were characterized electrochemically through monolayer reductive desorption and evaluation of the redox properties of the immobilized molecules in organic medium, and by scanning tunneling microscopy (STM). It was found that after assembly the Co(II) porphyrin is electroactive exhibiting the typical redox processes observed for its precursor without the appended lipoic acid in solution. A coverage of 2.7 × 10-10 mol.cm -2 has been estimated assuming that four electrons (one per each sulfur atom) are involved in the process. The porphyrin-modified gold electrodes exhibit catalytic acitivity demonstrated towards the reduction of molecular oxygen in acidic solution.

scholarly journals Self-Assembly of Pyridine-Modified Lipoic Acid Derivatives on Gold and Their Interaction with Thyroxine (T4)

2013 ◽  
Vol 14 (2) ◽  
pp. 3500-3513 ◽  
Author(s):  
Willem Albers ◽  
Roberto Milani ◽  
Kirsi Tappura ◽  
Tony Munter ◽  
Giuseppe Resnati ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Self Assembly

Self-assembly of a Ru(II)-deuteroporphyrin lipoic acid derivative on Au(111) surfaces

2015 ◽  
Vol 19 (09) ◽  
pp. 1014-1020 ◽  
Author(s):  
Rudy Martin-Trasanco ◽  
Roberto Cao ◽  
Franz-Peter Montforts
Keyword(s):  
Cyclic Voltammetry ◽  
Acid Derivative ◽  
Lipoic Acid ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Gold Electrode ◽  
Reduction Peak ◽  
X Ray ◽  
Irreversible Reduction ◽  
Reductive Desorption

The synthesis of a new carbonyl ruthenium(II) deuteroporphyrin-IX lipoic acid derivative (Ru(PLip)(CO)) (4) and its self-assembly on Au(111) surfaces were accomplished. Ru(PLip)(CO) 4 was prepared by reduction of the ester groups of carbonyl-ruthenium(II) deuteroporphyrindimethylester 1 and further esterification with D,L-α-lipoic acid 3. The self-assembly of Ru(PLip)(CO) 4 was confirmed by X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry (CV). The S2p XP spectrum of SAM formed by Ru(PLip)(CO) showed the S2p3/2 peak at 162.4 eV which corresponds to thiolated species bounded to gold. The influence of the interaction of porphyrin moieties on SAM stability was studied. The reductive desorption voltammogram of Ru(PLip)(CO) 4 self-assembled on gold showed an intense reduction peak at -1.02 V while when pyridine was coordinated to the central ruthenium(II) the reductive desorption peak was shifted to -0.85 V. The capacity of the modified Ru(PLip)(CO) gold electrode to detect nitric oxide (NO) was investigated by cyclic voltammetry. An irreversible reduction peak which increased with time on NO exposure was registered at -0.69 V indicating NO coordination to ruthenium(II).

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

The Nature of Rapidly Extracted Phycocyanin from the Blue-Green Alga Plectonema Boryanum

1971 ◽  
Vol 29 ◽  
pp. 366-367
Author(s):  
M. Kessel ◽  
R. MacColl
Keyword(s):  
Self Assembly ◽  
Analytical Ultracentrifugation ◽  
Uranyl Acetate ◽  
The Self ◽  
Major Protein ◽  
Subunit Structure ◽  
Blue Green Alga ◽  
Thin Sections ◽  
Blue Green Algae ◽  
Cacodylate Buffer

The major protein of the blue-green algae is the biliprotein, C-phycocyanin (Amax = 620 nm), which is presumed to exist in the cell in the form of distinct aggregates called phycobilisomes. The self-assembly of C-phycocyanin from monomer to hexamer has been extensively studied, but the proposed next step in the assembly of a phycobilisome, the formation of 19s subunits, is completely unknown. We have used electron microscopy and analytical ultracentrifugation in combination with a method for rapid and gentle extraction of phycocyanin to study its subunit structure and assembly.To establish the existence of phycobilisomes, cells of P. boryanum in the log phase of growth, growing at a light intensity of 200 foot candles, were fixed in 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.0, for 3 hours at 4°C. The cells were post-fixed in 1% OsO4 in the same buffer overnight. Material was stained for 1 hour in uranyl acetate (1%), dehydrated and embedded in araldite and examined in thin sections.

Video real-time imaging of artificial membranes during freeze-drying by cryo-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 16-17
Author(s):  
Alan S. Rudolph ◽  
Ronald R. Price
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Freeze Drying ◽  
Video Capture ◽  
Drying Process ◽  
Artificial Membranes ◽  
The Past ◽  
Cryo Electron Microscopy ◽  
Spherical Structures ◽  
Capture Techniques

We have employed cryoelectron microscopy to visualize events that occur during the freeze-drying of artificial membranes by employing real time video capture techniques. Artificial membranes or liposomes which are spherical structures within internal aqueous space are stabilized by water which provides the driving force for spontaneous self-assembly of these structures. Previous assays of damage to these structures which are induced by freeze drying reveal that the two principal deleterious events that occur are 1) fusion of liposomes and 2) leakage of contents trapped within the liposome [1]. In the past the only way to access these events was to examine the liposomes following the dehydration event. This technique allows the event to be monitored in real time as the liposomes destabilize and as water is sublimed at cryo temperatures in the vacuum of the microscope. The method by which liposomes are compromised by freeze-drying are largely unknown. This technique has shown that cryo-protectants such as glycerol and carbohydrates are able to maintain liposomal structure throughout the drying process.

Biomimetic materials: An introduction

1992 ◽  
Vol 50 (2) ◽  
pp. 1020-1021 ◽  
Author(s):  
M. Sarikaya ◽  
J. T. Staley ◽  
I. A. Aksay
Keyword(s):  
Self Assembly ◽  
Structural Control ◽  
Hierarchical Structures ◽  
Ceramic Composites ◽  
Advanced Materials ◽  
Length Scale ◽  
Spider Webs ◽  
Biomimetic Materials ◽  
Synthetic Materials ◽  
All Ceramic

Biomimetics is an area of research in which the analysis of structures and functions of natural materials provide a source of inspiration for design and processing concepts for novel synthetic materials. Through biomimetics, it may be possible to establish structural control on a continuous length scale, resulting in superior structures able to withstand the requirements placed upon advanced materials. It is well recognized that biological systems efficiently produce complex and hierarchical structures on the molecular, micrometer, and macro scales with unique properties, and with greater structural control than is possible with synthetic materials. The dynamism of these systems allows the collection and transport of constituents; the nucleation, configuration, and growth of new structures by self-assembly; and the repair and replacement of old and damaged components. These materials include all-organic components such as spider webs and insect cuticles (Fig. 1); inorganic-organic composites, such as seashells (Fig. 2) and bones; all-ceramic composites, such as sea urchin teeth, spines, and other skeletal units (Fig. 3); and inorganic ultrafine magnetic and semiconducting particles produced by bacteria and algae, respectively (Fig. 4).

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