scholarly journals Effect of leaf extract buasbuas (Premna pubescens Blume) for against of bacteria growth Staphylococcus aureus and Salmonella sp in vitro

2017 ◽  
Author(s):  
Martina Restuati ◽  
Nanda Pratiwi ◽  
Gita Widiyastuti
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Bacteria Growth

scholarly journals Uji daya hambat ekstrak daun stevia (Stevia rebaudiana Bertoni M.) terhadap pertumbuhan Staphylococcus aureus secara in vitro

e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Yaromis Wenda ◽  
Pemsi M. Wowor ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Stevia Rebaudiana ◽  
Inhibition Zone ◽  
Group Design ◽  
Stevia Rebaudiana Bertoni ◽  
Post Test ◽  
Experimental Laboratory ◽  
Active Substances

Abstract: Currently, oral health requires comprehensive handling by dentists and other health professionals. Osteomyelitis is an infection that occurs in bone tissue and bone marrow of the jaw and/or cortical bones, predominantly caused by Staphylococcus aureus. Medicinal plants in Indonesia have been widely used as traditional medicine inter alia stevia (Stevia rebaudiana Bertoni M.). This stevia plant has components that are typical natural sweetener and stevioside which has the characteristics as antibacterial, antiviral, anti-inflammatory, antimicrobial, and antifungal, and other active substances including tannins, alkaloids, flavonoids, and phenol. This was an experimental laboratory study with the post test only group design. Subjects were Staphylococcus aureus bacteria. The results showed that stevia leaf extract had inhibition zone to Staphylococcus aureus growth of 10.32 mm which was categorized as strong according to Davis and Stout 1971. Conclusion: Stevia leaf extract (Stevia rebaudiana Berrtoni M.) had strong inhibition to Staphylococcus aureus.Keywords: osteomyelitis, stevia (Stevia rebaudiana Bertoni M.), Staphylococcus aureusAbstrak: Dewasa ini kesehatan gigi dan mulut memerlukan penanganan secara komperhensif oleh dokter gigi maupun tenaga kesehatan lainnya. Salah satu penyakit yang sering dijumpai yaitu osteomielitis pada jaringan tulang dan sum-sum tulang rahang dan/atau korteks tulang dengan penyebab utama ialah bakteri Staphylococcus aureus. Tanaman herbal di Indonesia telah banyak digunakan sebagai obat tradisional; salah satunya ialah tanaman stevia (Stevia rebaudiana Bertoni M.). Tanaman stevia memiliki komponen yang bersifat pemanis alami, stevioside yang berefek antibakteri, antivirus, antiinflamasi, antifungsi, dan antimikroba, serta zat aktif di antaranya ialah tannin, alkaloid, flavonoid, dan fenol. Jenis penelitian ini ialah eksperimental laboratorik dengan post test only group design. Subyek penelitian ialah bakteri Staphylococcus aureus. Hasil penelitian memperlihatkan bahwa ekstrak daun stevia (Stevia rebaudiana Bertoni M.) mempunyai rerata zona hambat terhadap pertumbuhan bakteri Staphylococcus aureus sebesar 10,32 mm yang tergolong kuat menurut Davis dan Stout 1971. Simpulan: Ekstrak daun stevia (Stevia rebaudiana Berrtoni M.) memiliki daya hambat yang kuat terhadap bakteri Staphylococcus aureus.Kata kunci: osteomielitis, stevia (Stevia rebaudiana Bertoni M.), Staphylococcus aureus

scholarly journals Antimicrobial Activity of Nisin and Lysozyme on Foodborne Pathogens Listeria Monocytogenes, Staphylococcus Aureus, Salmonella Typhimurium, and Escherichia Coli at Different pH

2018 ◽  
Author(s):  
Hamdollah Moshtaghi ◽  
Azadeh Rashidimehr ◽  
Behzad Shareghi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Inhibitory Concentration ◽  
Low Ph ◽  
Bacteria Growth ◽  
Microdilution Method ◽  
Natural Preservatives

Background: To prevent or inhibit the growth of pathogenic microorganisms and food spoilage factors, many studies have been done by using natural preservatives. The aim of study was to investigate the effect of different concentrations of lysozyme and Nisin on the growth rate and also to determine the minimum inhibitory concentration (MIC) and minimum bactericidal cocentratiin (MBC) of these combinations on the bacteria of Escherichia coli, Staphylococcus aureus, Salmonella typhimorium and Listeria monocytogenes. Methods: In this study, various concentrations of lysozyme and Nisin were set in form of alone concentration and in combination concentrations (0, 19.53, 39.06, 78.13, 156.25, 312.5, 625, 1250, 2500, 5000) in vitro conditions and 6 pH 5.5, 6, 6.5, 7, 7.5, and 8. Microdilution method at 24°C was done and the combined effect on bacteria growth was read by using ELISA reader. Results: The results showed that lysozyme was less effective on Escherichia coli and Nisin was less effective on Listeria monocytogenes. Moreover, combining lysozyme and Nisin at low pH decreased the MIC. Conclusions: The results of the study showed that the effect of combining lysozyme and Nisin on Staphylococcus aureus is above all other bacteria and at low pH reduces the MIC.

scholarly journals Effect of Ultraviolet Light Irradiation Combined with Riboflavin on Different Bacterial Pathogens from Ocular Surface Infection

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Jing Shen ◽  
Qingfeng Liang ◽  
Guanyu Su ◽  
Yang Zhang ◽  
Zhiqun Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Uv Light ◽  
Irradiation Time ◽  
Survival Rates ◽  
Experimental Conditions ◽  
Light Power ◽  
Bacteria Growth ◽  
Inhibition Effect ◽  
Staphylococcus Epidermis

In order to study Staphylococcus epidermis and Staphylococcus aureus in vitro viability after the exposure to ultraviolet (UV) light and riboflavin, twelve strains of Staphylococcus epidermis and twelve strains of Staphylococcus aureus were isolated from patients with bacterial keratitis. The growth situation of Staphylococcus epidermidis and Staphylococcus aureus under different experimental conditions was qualitatively observed. The number of colonies surviving bacteria was counted under different UV light power and different exposure time. The experiment showed that there was no inhibition effect on the growth of bacteria using riboflavin alone. In UV alone group and UV-riboflavin group, inhibition effect on the bacteria growth was found. The UV-riboflavin combination had better inhibition effect on bacteria than UV irradiation alone. The amount of bacteria in the UV-riboflavin group was decreased by 99.1%~99.5% and 54.8%~64.6% in the UV alone group, when the UV light power was 10.052 mW/cm2 and the irradiation time was 30 min. Moreover, with the increase of the UV power or irradiation time, the survival rates of bacteria were rapidly reduced. Compared with Staphylococcus aureus, Staphylococcus epidermis was more easily to be killed under the action of UV light combined with riboflavin.

scholarly journals Konsentrasi Hambat Minimum Ekstrak Daun Sawo (Achras Zapota L.) Terhadap Pertumbuhan Staphylococcus Aureus Secara In Vitro

BioScience ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 53
Author(s):  
Novia Yunika
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Test Tube ◽  
Study Results ◽  
West Sumatra ◽  
Negative Controls

Sapodilla (Achras zapota L.) is a plant commonly found on the island of Java, West Sumatra and West Nusa Tenggara. Sapodilla plant contains flavonoids, saponins and tannins. In leaf the dominant are flavonoids. The content of flavonoids in the plant tissue may act as an antioxidant and antibacterial. As an alternative natural antibacterial, sapodilla leaves should also be tested minimum inhibitory concentration effective to inhibit the bacteria Staphylococcus aureus. This study aims to determine the minimum inhibitory concentration sapodilla leaf extract (Achras zapota L.) on the growth of Staphylococcus aureus. The research was conducted from January to February 2015 in the Laboratory of Microbiology  UNP. Research method is descriptive by observing turbidity levels at each test tube as well as comparison increment Optical Density (OD) before incubation and after incubation of zeroes. Sapodilla leaf extract concentration ranging from 10% - 50%, amoxilin as a positive control, and negative controls (without extract). Study results showed that the minimum inhibitory concentration of Sapodilla leaf extract on the growthbacteria present in a concentration of 50%. Keywords : Sapodilla leaf extract , Minimun Inhibitory Concentration (MIC), and Staphylococcus aureus

scholarly journals Antimicrobial and Antibiofilm Activities of Methanol Leaf Extract of Citrus maxima against Clinical Isolates of Multidrug Resistant Staphylococcus aureus

2021 ◽  
Author(s):  
Sanjeev Kumar ◽  
Arup Kumar Samanta ◽  
P. Roychoudhury ◽  
Honeysmita Das ◽  
Kalyan Sarma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Plant Extracts ◽  
Leaf Extract ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Zone Of Inhibition ◽  
Citrus Maxima ◽  
Mic Value

Background: Mitigation process to curb the ever increasing problem of antimicrobial resistance through development of new class of antimicrobials is slow and costly affairs. Research on alternative to conventional antimicrobials using plant based products as good source of numerous phytochemicals have potential to cope up the antimicrobial resistance. The present study was formulated on detection of in vitro antimicrobial and antibiofilm properties of methanol leaf extract of Citrus maxima against clinical isolates of Staphylococcus aureus. Methods: Leaves of Citrus maxima plants were collected from the campus of College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Aizawl, Mizoram and processed for preparation of methanol crude extract. The plant extracts were evaluated for their phytochemical and antioxidant properties using DPPH (2, 2-Diphenyl-1-picrylhydrazyl) method. Twenty well characterized biofilm producing and multidrug resistant Staphylococcus aureus strains recovered from milk of mastitic cows from Mizoram were received from the cultural repository of the department. The plant extracts were subjected to determine their antimicrobial and antibiofilm activities against all the bacterial isolates including S. aureus (ATCC 29213) by in vitro agar well diffusion method and 96 well microtiter plate methods, respectively. The MIC value of the plant extracts were determined by microdilution method. Result: In the methanol leaf extract of C. maxima alkaloids, glycosides, terpenoids, tannin and phenol and flavonoids were detected by qualitative analysis. Saponin, protein, free amino acids, steroids and carbohydrates were not detected. The free radical scavenging potential of the extract was found to be 10.66±1.84% to 36.10±1.98%, which was comparatively lower than ascorbic acid (83.39±0.13% to 89.76±0.24%). A total of 8 (40.0%), 5 (25.0%) and 7 (35.0%) strains were recorded as weak, moderate and strong biofilm producer. Maximum antibacterial activity against standard culture was observed with the zone of inhibition of 18 mm at 200 mg/mL concentration and MIC value at 25 mg/mL. Maximum antimicrobial activities against clinical isolates were recorded with 11.8±1.13 mm zone of inhibition at 200 mg/mL and MIC value at 25 mg/mL. The clinical isolates exhibited highest (85.94±1.00%) biofilm inhibition at 6.25 mg/mL. To the best of our knowledge, this is the first-ever report on antibiofilm and antioxidative activities of C. maxima leaf extracts against any bacteria.

scholarly journals Susceptibility Assessment of Methicillin-Resistant Staphylococcus aureus Strains to Lepidium sativum Extract

Dose-Response ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 155932581985042
Author(s):  
Amna A. Saddiq ◽  
Azza M. Mohamed
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Exposure Period ◽  
Inhibition Zone ◽  
Lepidium Sativum ◽  
Susceptibility Assessment ◽  
Methicillin Resistant ◽  
Bacteria Growth ◽  
Microbial Infections

Many plant-derived compounds have been used to treat microbial infections. Staphylococcus aureus a common cause of many organ infections, has generated increasing concern due to its resistance to antibacterial drugs. This work was carried out to explore the susceptibility of 6 strains (LN872136, LN872137, LN871238, LN871239, LN872140, and LN871241) of methicillin-resistant Staphylococcus aureus to aqueous extract of Lepidium sativum seeds in vitro. Various concentrations (5-20 mg/mL) were used to evaluate the effect of the extract on bacteria growth via the assessment of the microbial biomass and the inhibition zone (IZ). The results showed that the plant extract at 15 or 20 mg/mL, significantly decreased the the biomass of S aureus strains after 24 or 48 hours exposure period. Staphylococcus aureus (LN871241) showed the largest IZ at 20 mg/mL and documented by scanning electron microscope. The current work may suggest that L sativum seed extract can be candidate as a promising antimicrobial agent to treat infection with methicillin-resistant S aureus.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Ketapang (Terminalia catappa L.) Warna Hijau dan Warna Merah serta Kombinasinya

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Munira Munira Munira ◽  
Rasidah Rasidah Rasidah ◽  
Eva Melani Melani ◽  
Noni Zakiah Zakiah ◽  
Muhammad Nasir Nasir
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Green Leaf ◽  
Diffusion Method ◽  
Bacteria Growth ◽  
Terminalia Catappa

ABSTRAKDaun ketapang (Terminalia catappa L.) adalah tanaman yang sering dimanfaatkan oleh masyarakat sebagai tanaman obat tradisional. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak etanol daun ketapang warna hijau, warna merah, kombinasi daun warna hijau dan daun warna merah terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P0 (aquadest sebagai kontrol negatif), P1 (ekstrak etanol daun hijau), P2 (ekstrak etanol daun merah), dan P3 (kombinasi ekstrak etanol daun ketapang berwarna hijau dan merah) dan masing-masing 3 kali ulangan. Uji mikrobiologi menggunakan metode difusi. Berdasarkan hasil uji Anova menunjukkan bahwa ekstrak daun ketapang sangat berpengaruh terhadap pertumbuhan Staphylococcus aureus (P=0,000).Berdasarkan hasil uji lanjut Duncan rata-rata diameter zona hambat yang terbentuk menunjukkan bahwa kombinasi ekstrak daun ketapang warna hijau dan warna merah memiliki zona hambat yang lebih besar (30,92 mm) dan berbeda nyata dengan ekstrak daun ketapang berwarna hijau (21,92 mm), akan tetapi tidak berbeda nyata dengan ekstrak daun ketapang warna merah (26,42 mm).Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak daun ketapang dapat menghambat pertumbuhan bakteri Staphylococcus aureus.Kata kunci: Terminalia catappa L., diameter zona hambat, Staphylococcus aureus Ketapang leaf (Terminalia catappa L.) is a plant that is often used by the community as a traditional medicinal plant. This research was conducted to determine the effect of ethanol extract of green ketapang leaves, red color, combination of green leaf and red leaf color to Staphylococcus aureus bacteria growth. This research was experimental by using Completely Randomized Design (RAL) with 4 treatments which is  P0 (aquadest as negative control), P1 (green leaf ethanol extract), P2 (red leaf ethanol extract), and P3 (combination of green leaf ketapang ethanol extract and red) and each of them repeated 3 times. Microbiology test using diffusion method. Based on the results of Anova test showed that ketapang leaf extract greatly affect the growth of Staphylococcus aureus (P=0.000). Based on the results of further test Duncan average diameter of the inhibition zone that was formed showed that the combination of green leaf and red colored leaf extract has a larger inhibition zone (30.92 mm) and significantly different with green leaf ketapang leaf extract (21.92 mm), but not significantly different with the extract of red leaf ketapang (26.42 mm). From the results of this study can be concluded that ketapang leaf extract can inhibit the growth of Staphylococcus aureus.Keywords: Terminalia catappa L., inhibition zone diameter, Staphylococcus aureus 

scholarly journals Caerin 1.1 and 1.9 peptides from Australian tree frog inhibit antibiotic-resistant bacteria growth in a murine skin infection model

2021 ◽  
Author(s):  
Shu Chen ◽  
Pingping Zhang ◽  
Liyin Xiao ◽  
Ying Liu ◽  
Kuihai Wu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
Bacterial Resistance ◽  
Resistant Bacteria ◽  
Infection Model ◽  
Temperature Sensitive ◽  
Tree Frog ◽  
Bacteria Growth ◽  
Wide Range

AbstractHost-defence caerin 1.9 peptide was originally isolated from skin secretion of Australian tree frog, and inhibits the growth of a wide range of bacteria in vitro. In this study, we demonstrated that caerin 1.9 shows high bioactivity against several bacteria strains, such as Staphylococcus aureus, Acinetobacter Baumannii, methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus hemolyiicus in vitro. Importantly, unlike antibiotic Tazocin, caerin 1.9 does not induce bacterial resistance after 30 rounds of in vitro culture. Moreover, caerin 1.1, another peptide of caerin family, has additive antibacterial effect when used together with caerin 1.9. Furthermore, caerin 1.1 and 1.9 prepared in the form of a temperature sensitive gel inhibit MRSA growth in skin bacterial infection model of two murine strains. These results indicate that caerin 1.1 and 1.9 may have the advantage than conventional antibiotics against bacterial infection of skin.

scholarly journals Aktivitas antibakteri salep ekstrak etanol daun sirih hijau (Piper betleL.) Terhadap infeksi bakteri Staphylococcus aureus

2018 ◽  
Vol 5 (2) ◽  
pp. 56
Author(s):  
Rissa Laila Vifta ◽  
Muhammad Andri Wansyah ◽  
Anita Kumala Hati
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Piper Betle ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Betel Leaf

<p align="center"><strong>Abstrak </strong></p><p align="center"><strong> </strong></p><p>Sirih hijau (<em>Piper betle L</em>.) adalah tanaman yang mudah dijumpai di Indonesia. Secara empiris, sirih hijau  digunakan sebagai antibakteri pada luka. Penelitian ini bertujuan untuk mengetahui aktivitas salep ekstrak etanol daun sirih hijau sebagai antibakteri. Pengujian efek antibakteri dilakukan secara <em>in vitro </em>dan <em>in vivo</em>. Ekstrak etanol daun sirih hijau dibuat dengan cara maserasi. Pengujian secara <em>in vitro</em> dilakukan menggunakan metode mikrodilusi terhadap <em>Staphylococcus aureus</em>  untuk mengetahui nilai konsentrasi hambat minimum ekstrak daun sirih hijau pada konsentrasi 3, 4, dan 5%.  Kemudian ekstrak dibuat salep dan diuji secara <em>in vivo</em> menggunakan hewan uji tikus yang terdiri dari kelompok kontrol negatif, kelompok ekstrak (konsentrasi 3,4, dan 5%), serata pembanding kontrol positif gentamisin 0,1%. Hasil uji dianalisis menggunakan <em>Sapiro-Wilk</em> dan dilanjutkan dengan uji ANAVA serta uji LSD untuk mengetahui perbandingan hasil kelima kelompok perlakuan. Hasil uji <em>in vitro</em> menunjukkan bahwa konsentrasi hambat minimum ekstrak daun sirih yang optimal adalah 5%. Hasil uji <em>in vivo</em> menunjukkan lama kesembuhan luka meningkat seiring dengan bertambahnya konsentrasi ekstrak daun sirih hijau. Hasil uji normalitas, Anava, dan uji LSD pada uji <em>in vivo</em> memberikan hasil yang sejalan. Konsentrasi 4 dan 5% merupakan konsentrasi efektif dengan aktivitas waktu kesembuhan luka tidak berbeda bermakna dengan kontrol positif (gentamisin 0,1%) dengan rerata waktu berturut-turut 6,20±0,80 dan 6,00±0,71 hari. Dapat disimpulkan bahwa hasil uji antibakteri pada salep sejalan dengan uji secara <em>in-vitro</em> ekstrak daun Sirih Hijau yang memiliki potensi sebagai antibakteri.</p><p><strong>Kata kunci:</strong>     Daun sirih hijau, Antibakteri, Salep, Luka</p><p> </p><p align="center"><strong><em>Antibacterial activity from ointment contains extract of green betle leaves (Piper betle l.) to Staphylococcus aureus bacterial infection</em></strong></p><p align="center"><strong> </strong></p><p align="center"><strong><em>Abstract </em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Green betel (Piper betle L.) is a plant that is easy to find in Indonesia. Empirically, green betel is used as an antibacterial to the wound. This study aims to determine the activity of ointment </em><em>of </em><em>ethanol extract of green </em><em>betel</em><em> leaves as antibacterial. Tests of antibacterial effects were performed in vitro and in vivo. Ethanol extract of green betel leaf is made by maceration. In vitro testing was done using micro dilution method to Staphylococcus aureus to determine the minimum inhibitory concentration of green leaf extract at concentrations of 3, 4, and 5%. Then the extract was made ointment and tested in vivo using rat consisting of the negative control group, the extract group (concentrations 3,4, and 5%), the positive control of gentamicin 0.1%. The test results were analyzed using Sapiro-Wilk and continued with ANAVA test and LSD test to find out the comparison of the results of the five treatment groups. The results of in vitro test showed that the minimum inhibit concentration of </em><em>green </em><em>betel leaf extract was 5%. The results of in vivo test showed the duration of wound healing increased with increasing concentration of green betel leaf extract. The result of normality test, Anava, and LSD test on in vivo test give the same result. Concentrations of 4 and 5% were effective concentrations with wound healing time activity were not significantly different with positive control (gentamicin 0.1%) with mean time of 6.20 ± 0.80 and 6.00 ± 0.71 days respectively. It can be concluded that the antibacterial test results in ointment are in line with in vitro test of leaf </em><em>green betel </em><em>extract which has potential as antibacterial.</em><strong><em></em></strong></p><p><strong><em>Key words</em></strong><em>:      Green betel, Antibacterial, Ointment, Wound</em></p>

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