scholarly journals The role of shear stress and altered tissue properties on endothelial to mesenchymal transformation and tumor-endothelial cell interaction

2017 ◽  
Vol 11 (4) ◽  
pp. 044104 ◽  
Author(s):  
Sara G. Mina ◽  
Peter Huang ◽  
Bruce T. Murray ◽  
Gretchen J. Mahler
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Interaction ◽  
Tissue Properties ◽  
Endothelial Cell Interaction ◽  
Tumor Endothelial Cell ◽  
Mesenchymal Transformation

Integrated Microfluidic Platform with Multiple Functions To Probe Tumor–Endothelial Cell Interaction

2017 ◽  
Vol 89 (18) ◽  
pp. 10037-10044 ◽  
Author(s):  
Ling Lin ◽  
Xuexia Lin ◽  
Luyao Lin ◽  
Qiang Feng ◽  
Takehiko Kitamori ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Microfluidic Platform ◽  
Multiple Functions ◽  
Endothelial Cell Interaction ◽  
Tumor Endothelial Cell

Tumor-endothelial cell interaction in an experimental model of human hepatocellular carcinoma

2018 ◽  
Vol 372 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Azemat Jamshidi-Parsian ◽  
Robert J. Griffin ◽  
Rajshekhar A. Kore ◽  
Valentina K. Todorova ◽  
Issam Makhoul
Keyword(s):  
Hepatocellular Carcinoma ◽  
Endothelial Cell ◽  
Experimental Model ◽  
Cell Interaction ◽  
Human Hepatocellular Carcinoma ◽  
Endothelial Cell Interaction ◽  
Tumor Endothelial Cell

Platelet-endothelial cell interaction in pulmonary microcirculation: the role of PARS

2004 ◽  
Vol 91 (04) ◽  
pp. 761-770 ◽  
Author(s):  
Rainer Kiefmann ◽  
Kai Heckel ◽  
Sonja Schenkat ◽  
Martina Dörger ◽  
Józefa Węsierska-Gądek ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Interaction ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelium ◽  
Endotoxin Infusion ◽  
Endothelial Cell Interaction ◽  
Pulmonary Microcirculation ◽  
Kinetics Of

SummaryAccumulation of platelets might contribute to acute lung injury during systemic inflammation. The aim of the study was to elucidate the role of the poly (ADP-ribose) synthetase, a nucleotide-polymerizising enzyme, in mediation of platelet-endothelial cell interaction through regulation of adhesion molecules within the pulmonary microcirculation during endotoxemia. We used in vivo fluorescence microscopy to quantify kinetics of fluorescently labeled erythrocytes and platelets in rabbit pulmonary arterioles and venules. Six hours after onset of endotoxin infusion we observed a massive interaction of platelets with the microvascular endothelial cells, whereas under control conditions, no platelet sequestration was measured. An up-regulation of P- and E-selectin was detected in lung tissue following endotoxin infusion by immunohistochemistry and Western blot analysis. Blockade of endothelial P-selectin with fucoidin resulted in a reduction of the endotoxin-induced platelet-endothelial cell interaction. Inhibition of poly (ADP-ribose) synthetase by 3-aminobenzamide inhibited the endotoxin-induced expression of endothelial P- and E-selectin and the subsequent recruitment of platelets. In summary, we provide first in vivo evidence that platelets accumulate in pulmonary microcirculation following endotoxemia. Poly (ADP-ribose) synthetase seems to mediate this platelet-endothelial cell interaction via P- and E-selectin expressed on the surface of microvascular endothelium.

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