Implementation of [18F]GE-180 for routine production on ORA Neptis Perform Synthesizer®

2017 ◽  
Author(s):  
S. Jivan ◽  
G. Villeret ◽  
K. Neumannn ◽  
J. Slater ◽  
H. Vanbrocklin
Keyword(s):  

In the years since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The basic problems outstanding at the time of publication of this earlier work have now been, for the most part, solved. As a result, innovators in the field have focussed their efforts to develop methodologies and chemistry for the synthesis of more complex structures. The focus of this new volume is much broader, and covers not only the essential procedures for the production of linear peptides but also more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods also deserving attention have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is also covered, as well as methods for overcoming aggregation during peptide chain assembly and a survey of available automated instrumentation.


1982 ◽  
Vol 9 (3) ◽  
pp. 218
Author(s):  
K.H. Assmus ◽  
W. Maier ◽  
R. Schutz ◽  
F. Schulz ◽  
H. Schweickert

2008 ◽  
Vol 51 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Vanessa Gómez ◽  
Juan Domingo Gispert ◽  
Víctor Amador ◽  
Jordi Llop

2013 ◽  
Vol 76 ◽  
pp. 46-54 ◽  
Author(s):  
Michael K. Schultz ◽  
Dirk Mueller ◽  
Richard P. Baum ◽  
G. Leonard Watkins ◽  
Wouter A.P. Breeman

1989 ◽  
Vol 36 (5) ◽  
pp. 473-490 ◽  
Author(s):  
Scott Coltrane

2008 ◽  
Vol 66 (3) ◽  
pp. 295-302 ◽  
Author(s):  
Mario Marengo ◽  
Filippo Lodi ◽  
Silvia Magi ◽  
Gianfranco Cicoria ◽  
Davide Pancaldi ◽  
...  
Keyword(s):  

Zygote ◽  
2013 ◽  
Vol 22 (3) ◽  
pp. 356-365 ◽  
Author(s):  
Juan Li ◽  
Hanne Skovsgaard Pedersen ◽  
Rong Li ◽  
Janne Adamsen ◽  
Ying Liu ◽  
...  

SummaryIn this study, the developmental ability of cloned embryos using gilt versus sow oocytes was evaluated under the hypothesis that the efficiency of nuclear transfer using gilt oocytes was lower than that of sow oocytes, but that it could be optimized. Five experiments were performed with routine production of cloned embryos with sow oocytes serving as the control. Results showed that: Experiment 1: Blastocyst rates of cloned embryos with gilt oocytes was about half compared with control. Experiment 2: An extended maturation time of 48 h used for gilt oocytes resulted in lower blastocyst rates after cloning. Experiment 3: Development of cloned embryos with gilt oocytes was improved by co-culture with sow oocytes. Experiment 4: After maturation of gilt oocytes using follicular fluid from gilt instead of sow, the oocytes were sorted into large and small oocytes, and after cloning, blastocyst rates were higher using large gilt oocytes compared with small oocytes; however, the rate remained lower compared with control. Experiment 5: Six sow recipients received a total of 503 morulae and blastocysts cloned from gilt oocytes (four recipients) and 190 cloned from sow oocytes (two recipients). All recipients became pregnant and went to term, resulting in 26 (gilt oocytes) and six (sow oocytes) piglets. In conclusion, results confirmed that nuclear transfer efficiency was higher using sow versus gilt oocytes, but the use of gilt oocytes can be optimized by sorting after ooplasm size following maturation and by maturing gilt and sow oocytes together.


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