scholarly journals X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization

2017 ◽  
Vol 11 (2) ◽  
pp. 024118 ◽  
Author(s):  
Jeremy M. Schieferstein ◽  
Ashtamurthy S. Pawate ◽  
Chang Sun ◽  
Frank Wan ◽  
Paige N. Sheraden ◽  
...  
Keyword(s):  
Membrane Protein ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Crystallization ◽  
Microfluidic Chips ◽  
X Ray ◽  
Membrane Protein Crystallization

scholarly journals Characterization of lipid matrices for membrane protein crystallization by high-throughput small angle X-ray scattering

Methods ◽  
2011 ◽  
Vol 55 (4) ◽  
pp. 342-349 ◽  
Author(s):  
Jeremiah S. Joseph ◽  
Wei Liu ◽  
Joshua Kunken ◽  
Thomas M. Weiss ◽  
Hiro Tsuruta ◽  
...  
Keyword(s):  
Membrane Protein ◽  
High Throughput ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Ray Scattering

Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins

2003 ◽  
Vol 36 (6) ◽  
pp. 1372-1377 ◽  
Author(s):  
Vadim Cherezov ◽  
Martin Caffrey
Keyword(s):  
Optical Properties ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
High Throughput ◽  
High Throughput Screening ◽  
Low Cost ◽  
Protein Crystallization ◽  
Batch Crystallization ◽  
Membrane Protein Crystallization ◽  
Crystallization Trial

A simple convenient and low-cost glass-based plate for high-throughput screening of membrane protein crystallization is described. The plates are robust and reduce dramatically the amount of protein and precipitant solution used per crystallization trial, while offering excellent optical properties for the detection of micro-crystals and crystals of colorless proteins. The plates were developed primarily for crystallization of membrane proteins in lipidic mesophases. They can also be used in batch crystallization of soluble and membrane proteins.

scholarly journals Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

2015 ◽  
Vol 71 (7) ◽  
pp. 838-846 ◽  
Author(s):  
Mohamed Dahani ◽  
Laurie-Anne Barret ◽  
Simon Raynal ◽  
Colette Jungas ◽  
Pétra Pernot ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Membrane Protein ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Surfactant Phase ◽  
Ray Scattering

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

scholarly journals Development of an Automated High Throughput LCP-FRAP Assay to Guide Membrane Protein Crystallization in Lipid Mesophases

2011 ◽  
Vol 11 (4) ◽  
pp. 1193-1201 ◽  
Author(s):  
Fei Xu ◽  
Wei Liu ◽  
Michael A. Hanson ◽  
Raymond C. Stevens ◽  
Vadim Cherezov
Keyword(s):  
Membrane Protein ◽  
High Throughput ◽  
Protein Crystallization ◽  
Frap Assay ◽  
Membrane Protein Crystallization

Small-Angle X-ray Scattering Study of Photosystem I−Detergent Complexes:  Implications for Membrane Protein Crystallization

2007 ◽  
Vol 111 (16) ◽  
pp. 4211-4219 ◽  
Author(s):  
Hugh O'Neill ◽  
William T. Heller ◽  
Katherine E. Helton ◽  
Volker S. Urban ◽  
Elias Greenbaum
Keyword(s):  
Membrane Protein ◽  
Photosystem I ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Scattering Study ◽  
Ray Scattering

scholarly journals X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds

Lab on a Chip ◽  
2018 ◽  
Vol 18 (6) ◽  
pp. 944-954 ◽  
Author(s):  
Jeremy M. Schieferstein ◽  
Ashtamurthy S. Pawate ◽  
Michael J. Varel ◽  
Sudipto Guha ◽  
Ieva Astrauskaite ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Crystallization ◽  
Protein Crystals ◽  
Microfluidic Platforms ◽  
High Quality ◽  
X Ray ◽  
High Quality Protein ◽  
Membrane Protein Crystallization

Microfluidic platforms for formulation of crystallization trials with microseeds to enable the growth of high-quality protein crystals.

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