scholarly journals Hydroxyapatite reinforced with multi-walled carbon nanotubes and bovine serum albumin for bone substitute applications

2016 ◽  
Author(s):  
Fatemeh Gholami ◽  
Ahmad-Fauzi Mohd Noor
Keyword(s):  
Carbon Nanotubes ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Bone Substitute ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Scavenger Receptor A1 Mediates the Uptake of Carboxylated and Pristine Multi-Walled Carbon Nanotubes Coated with Bovine Serum Albumin

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 539
Author(s):  
Mai T. Huynh ◽  
Carole Mikoryak ◽  
Paul Pantano ◽  
Rockford Draper
Keyword(s):  
Carbon Nanotubes ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cho Cells ◽  
Bovine Serum ◽  
Scavenger Receptor ◽  
Chinese Hamster ◽  
Raw 264.7 ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Previously, we noted that carboxylated multi-walled carbon nanotubes (cMWNTs) coated with Pluronic® F-108 (PF108) bound to and were accumulated by macrophages, but that pristine multi-walled carbon nanotubes (pMWNTs) coated with PF108 were not (Wang et al., Nanotoxicology2018, 12, 677). Subsequent studies with Chinese hamster ovary (CHO) cells that overexpressed scavenger receptor A1 (SR-A1) and with macrophages derived from mice knocked out for SR-A1 provided evidence that SR-A1 was a receptor of PF108-cMWNTs (Wang et al., Nanomaterials (Basel) 2020, 10, 2417). Herein, we replaced the PF108 coat with bovine serum albumin (BSA) to investigate how a BSA corona affected the interaction of multi-walled carbon nanotubes (MWNTs) with cells. Both BSA-coated cMWNTs and pMWNTs bound to and were accumulated by RAW 264.7 macrophages, although the cells bound two times more BSA-coated cMWNT than pMWNTs. RAW 264.7 cells that were deleted for SR-A1 using CRISPR-Cas9 technology had markedly reduced binding and accumulation of both BSA-coated cMWNTs and pMWNTs, suggesting that SR-A1 was responsible for the uptake of both MWNT types. Moreover, CHO cells that ectopically expressed SR-A1 accumulated both MWNT types, whereas wild-type CHO cells did not. One model to explain these results is that SR-A1 can interact with two structural features of BSA-coated cMWNTs, one inherent to the oxidized nanotubes (such as COOH and other oxidized groups) and the other provided by the BSA corona; whereas SR-A1 only interacts with the BSA corona of BSA-pMWNTs.

scholarly journals Internalization, cytotoxicity, oxidative stress and inflammation of multi-walled carbon nanotubes in human endothelial cells: influence of pre-incubation with bovine serum albumin

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9253-9260 ◽  
Author(s):  
Jimin Long ◽  
Xianqiang Li ◽  
Yang Kang ◽  
Yanhuai Ding ◽  
Zhipeng Gu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Endothelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Blood ◽  
Bovine Serum ◽  
Human Endothelial Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

When entering circulation, multi-walled carbon nanotubes (MWCNTs) will inevitably adsorb proteins, which can consequently influence their toxicity to cells lining human blood vessels.

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