Lipid phase behavior studied with a quartz crystal microbalance: A technique for biophysical studies with applications in screening

2016 ◽  
Vol 145 (20) ◽  
pp. 204904 ◽  
Author(s):  
Astrid Peschel ◽  
Arne Langhoff ◽  
Eva Uhl ◽  
Aruna Dathathreyan ◽  
Susanne Haindl ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Phase ◽  
Biophysical Studies

scholarly journals Quartz Crystal Microbalance Monitoring of Poly(Vinyl Alcohol) Sol during the Freeze–Thaw Process

2016 ◽  
Vol 5 (3) ◽  
pp. 42
Author(s):  
Yoshimi Seida ◽  
Mitsuteru Ogawa
Keyword(s):  
Porous Structure ◽  
Phase Behavior ◽  
Quartz Crystal Microbalance ◽  
Vinyl Alcohol ◽  
Quartz Crystal ◽  
Free Water ◽  
Silica Gels ◽  
Poly Vinyl Alcohol ◽  
Freeze Thaw ◽  
Pore Size Distributions

<p class="1Body">A quartz crystal microbalance (QCM) working under cryo-conditions was applied to analyzing the gelation and/or phase behavior of poly(vinyl alcohol) (PVA) sol during repeated freeze–thaw processes. The development of a porous structure with the gelation of PVA sol during the freeze–thaw cycle was examined in terms of the thermal behavior of the water in the sol and the viscoelastic behavior of the sol through thermal and QCM analyses. Water was liberated from the hydrophilic PVA during the freeze–thaw process through the aggregation of PVA. The water decreased the freezing temperature and increased the melting temperature because of the development of the porous structure with gelation by the thermal treatment. The state of the water during the gelation was estimated from the phase transition temperature and enthalpy change of the water during the thermal scan by using water-saturated silica gels with a series of pore size distributions. The viscoelasticity of the PVA sol during the freeze–thaw process was measured by cryo-QCM using admittance analysis (QCM-A). The free water and/or porous structure in the PVA sol was found to increase from the viscoelastic point of view by QCM measurements showing a shift in the resonance parameters (<em>f</em><sub>s</sub>, <em>R</em><sub>1</sub>). A hard gel was confirmed to form by the decrease in <em>f</em><sub>s</sub> and the increase in <em>R</em><sub>1</sub> with the thermal scan treatment. The cryo-QCM was found to be an effective probe for clarifying the gelation and phase behavior of PVA sol in detail with high resolution.</p>

Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal

Effect of Current Distribution on Quartz Crystal Microbalance Measurements

1998 ◽  
Vol 145 (2) ◽  
pp. 492-497 ◽  
Author(s):  
James J. Kelly ◽  
K. M. Anisur Rahman ◽  
Christopher J. Durning ◽  
Alan C. West
Keyword(s):  
Quartz Crystal Microbalance ◽  
Current Distribution ◽  
Quartz Crystal

scholarly journals Unraveling cardiolipin-induced conformational change of cytochrome c through H/D exchange mass spectrometry and quartz crystal microbalance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sin-Cih Sun ◽  
Hung-Wei Huang ◽  
Yi-Ting Lo ◽  
Min-Chieh Chuang ◽  
Yuan-Hao Howard Hsu
Keyword(s):  
Mass Spectrometry ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Interaction Model ◽  
Moderate Increase ◽  
Exchange Mass Spectrometry ◽  
Mitochondrial Regulation ◽  
Crucial Component ◽  
Drastic Increase ◽  
Hydrogen Deuterium

AbstractCardiolipin (CL), a crucial component in inner mitochondrial membranes, interacts with cytochrome c (cyt c) to form a peroxidase complex for the catalysis of CL oxidation. Such interaction is pivotal to the mitochondrial regulation of apoptosis and is affected by the redox state of cyt c. In the present study, the redox-dependent interaction of cyt c with CL was investigated through amide hydrogen/deuterium exchange coupled with mass spectrometry (HDXMS) and quartz crystal microbalance with dissipation monitoring (QCM-D). Ferrous cyt c exhibited a more compact conformation compared with its ferric form, which was supported by the lower number of deuterons accumulated and the greater amplitude reduction on dissipation. Upon association with CL, ferrous cyt c resulted in a moderate increase in deuteration, whereas the ferric form caused a drastic increase of deuteration, which indicated that CL-bound ferric cyt c formed an extended conformation. These results were consistent with those of the frequency (f) − dissipation (D) experiments, which revealed that ferric cyt c yielded greater values of |ΔD/Δf| within the first minute. Further fragmentation analysis based on HDXMS indicated that the effect of CL binding was considerably different on ferric and ferrous cyt c in the C-helix and the Loop 9–24. In ferric cyt c, CL binding affected Met80 and destabilized His18 interaction with heme, which was not observed with ferrous cyt c. An interaction model was proposed to explain the aforementioned results.

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