scholarly journals Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic droplets

2016 ◽  
Vol 10 (5) ◽  
pp. 054115 ◽  
Author(s):  
S. Sarkar ◽  
P. Sabhachandani ◽  
D. Stroopinsky ◽  
K. Palmer ◽  
N. Cohen ◽  
...  
Keyword(s):  
Dynamic Analysis ◽  
Single Cell ◽  
Cancer Cell ◽  
Cell Interactions ◽  
Single Cell Level ◽  
Cell Level ◽  
Microfluidic Droplets

Single Cell Level Quantification of Nanoparticle–Cell Interactions Using Mass Cytometry

2017 ◽  
Vol 89 (16) ◽  
pp. 8228-8232 ◽  
Author(s):  
Angela Ivask ◽  
Andrew J. Mitchell ◽  
Christopher M. Hope ◽  
Simon C. Barry ◽  
Enzo Lombi ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Interactions ◽  
Single Cell Level ◽  
Mass Cytometry ◽  
Cell Level

DNA-fueled molecular machine for label-free and non-enzymatic ultrasensitive detection of telomerase activity

The Analyst ◽  
2016 ◽  
Vol 141 (16) ◽  
pp. 4855-4858 ◽  
Author(s):  
Panpan Sun ◽  
Xiang Ran ◽  
Chaoqun Liu ◽  
Chaoying Liu ◽  
Fang Pu ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cell ◽  
Telomerase Activity ◽  
Molecular Machine ◽  
Ultrasensitive Detection ◽  
Single Cell Level ◽  
Label Free ◽  
Enzymatic Method ◽  
Cell Level ◽  
Cell Extracts

A label-free and non-enzymatic method based on DNA-fueled molecular machine has been introduced for ultrasensitive detection of telomerase activity in cancer cell extracts even at the single-cell level.

scholarly journals Monitoring Bip promoter activation during cancer cell growth by bioluminescence imaging at the single-cell level

2016 ◽  
Vol 2 (6) ◽  
Author(s):  
Tomohisa Horibe ◽  
Aya Torisawa ◽  
Ryohsuke Kurihara ◽  
Ryutaro Akiyoshi ◽  
Yoko Hatta-Ohashi ◽  
...  
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Cancer Cell ◽  
Bioluminescence Imaging ◽  
Single Cell Level ◽  
Promoter Activation ◽  
Cell Level ◽  
Cancer Cell Growth

scholarly journals An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035

Photonics ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 533
Author(s):  
Pei-Wen Peng ◽  
Jen-Chang Yang ◽  
Mamadi M.S Colley ◽  
Tzu-Sen Yang
Keyword(s):  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitor ◽  
Single Cell ◽  
Cancer Cell ◽  
Kinase Inhibitor ◽  
Single Cell Level ◽  
Cell Level ◽  
Ht29 Cells ◽  
Coated Bead ◽  
Cell Chemotaxis

We presented an approach to address cancer cell chemotaxis and response to tyrosine kinase inhibitor PD153035 at the single-cell level. We applied an optical tweezer system together with the platform at the single-cell level to manipulate an epidermal growth factor (EGF)-coated bead positioned close to the filopodia to locally stimulate HT29 cells, the human colon cancer cell line overexpressing the EGF receptor (EGFR). To address cancer cell chemotaxis, a single-cell movement model was also proposed to quantify the propagation speed at the leading and trailing edges of the cell along the chemosensing axis. This study focused on three perspectives: probing the chemosensing process mediated by EGF/EGFR signaling, investigating the mode of locomotion during the EGF-coated bead stimulation, and quantifying the effect of PD153035 on the EGF–EGFR transport pathway. The results showed that the filopodial actin filament is a sensory system for EGF detection. In addition, HT29 cells may use the filopodial actin filament to distinguish the presence or absence of the chemoattractant EGF. Furthermore, we demonstrated the high selectivity of PD153035 for EGFR and the reversibility of binding to EGFR. We anticipate that the proposed single-cell method could be applied to construct a rapid screening method for the detection and therapeutic evaluation of many types of cancer during chemotaxis.

scholarly journals Probing Hexaminolevulinate Mediated PpIX Fluorescence in Cancer Cell Suspensions in the Presence of Chemical Adjuvants

2020 ◽  
Vol 21 (8) ◽  
pp. 2963 ◽  
Author(s):  
Kit Man Chan ◽  
Jonathan Gleadle ◽  
Krasimir Vasilev ◽  
Melanie MacGregor
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Protoporphyrin Ix ◽  
Cell Interaction ◽  
Cancer Diagnostics ◽  
Cell Suspensions ◽  
Single Cell Level ◽  
Cell Level ◽  
Suspended Cells

Exogenous administration of hexaminolevulinate (HAL) induces fluorescent protoporphyrin IX (PpIX) accumulation preferentially in cancer cells. However, the PpIX fluorescence intensities between noncancer and cancer cells are highly variable. The contrast between cancer and noncancer cells may be insufficient to reliably discriminate, especially at the single cell level in cancer diagnostics. This study examines the use of the chemical adjuvants dimethylsulphoxide (DMSO) or deferoxamine (DFO) to enhance the HAL induced PpIX accumulation in cancer cells. Our results showed that in some of the incubation conditions tested, the addition of DFO with HAL significantly increased PpIX 21 fluorescence of adherent monolayer cancer cells, but this was never the case for cells in suspension. Permeabilisation with DMSO did not increase PpIX fluorescence. Cell-to-cell interaction may well play an important role in the PpIX accumulation when suspended cells are treated in HAL and adjuvant chemicals.

scholarly journals TMIC-03. DISSECTING GLIOMA:IMMUNE CELL INTERACTIONS AT THE SINGLE CELL LEVEL

2018 ◽  
Vol 20 (suppl_6) ◽  
pp. vi256-vi256
Author(s):  
David Baskin ◽  
Joshy George ◽  
Krish Karuturi ◽  
Kyuson Yun
Keyword(s):  
Single Cell ◽  
Cell Interactions ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0139980 ◽  
Author(s):  
Marina Kobayashi ◽  
Soo Hyeon Kim ◽  
Hiroko Nakamura ◽  
Shohei Kaneda ◽  
Teruo Fujii
Keyword(s):  
Single Cell ◽  
Cancer Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Microwell Array
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