Interaction of bovine serum albumin protein with self assembled monolayer of mercaptoundecanoic acid

Author(s):  
Monika Poonia ◽  
Hitesh Agarwal ◽  
Manjuladevi V. ◽  
R. K. Gupta
2007 ◽  
Vol 561-565 ◽  
pp. 1529-1532
Author(s):  
Bo Feng ◽  
A. Liu ◽  
N. Wu ◽  
Jie Weng ◽  
Shu Xin Qu ◽  
...  

Bone-like hydroxyapatite (HA) coatings were fabricated on titanium implants by a self-assembled technique and biomimetic method. After titanium plates were oxidized in a mixture of H2SO4/H2O2, a hexadecanoic acid self-assembled onto titanium surfaces. Contact angles of water and tricresyl phosphate on the surfaces were measured to characterize the self-assembled monolayer (SAM) and confirm the presence of the functional group. The titanium plates with SAM were used to fabricate HA coatings. In the simulate body fluid (SBF) with and without bovine serum albumin (BSA), Ca2+ and PO4 3- ions could spontaneously deposit onto the titanium surfaces and form bone-like HA coatings. The morphology, component and structure of samples were examined by scanning electronic microscopy, X-ray photoelectron spectroscopy, X-ray diffraction and attenuated total reflection Fourier transform infrared spectroscopy. The results suggested that the SAM can induce the formation of the nano-HA coating with a network and microporous structure. For the biomimetical HA coating induced by HDA-SAM, BSA could modulate the growth of HA crystal and decreased the grain size.


2016 ◽  
Vol 8 (15) ◽  
pp. 9629-9634 ◽  
Author(s):  
Qiaoyu Yang ◽  
Zhongju Ye ◽  
Meile Zhong ◽  
Bo Chen ◽  
Jian Chen ◽  
...  

2017 ◽  
Vol 121 (17) ◽  
pp. 9579-9586 ◽  
Author(s):  
Edith Beilis ◽  
Yonatan Horowitz ◽  
Alon Givon ◽  
Gabor A. Somorjai ◽  
Hagai Cohen ◽  
...  

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Chenyu Su ◽  
Shanshan Liu ◽  
Shenghan Cao ◽  
Shuyan Yin ◽  
Chenggang Zhou ◽  
...  

Abstract Background Trunk-boring pests (TBPs) are an important type of forest pest, TBPs not only feed on the branches and trunks of trees, but also spread quarantine diseases in forests. However, because the larvae of TBPs live inside the trunk and are well concealed, prevention and control are difficult. The lack of effective control methods leads to the death of many trees in forests. In this study, a novel nanopesticide featuring high bioactivity and slow-release properties was developed to control TBPs. Thiacloprid (THI), which is commonly used to control Coleoptera species, was used as a model pesticide. Results The oleophobic properties of bovine serum albumin (BSA) were exploited to encapsulate the hydrophobic pesticide THI by self-assembly, and the size of the obtained nanoparticles, THI@BSA·NPs, was approximately 23 nm. The loading efficiency reached 70.4%, and THI@BSA·NPs could be released continuously for over 15 days, with the cumulative release reaching 93.5%. The fluorescein isothiocyanate (FITC)-labeled nanoparticles were evenly distributed in the digestive tract and body surface of a typical TBPs, M. alternatus, and the stomach and contact toxicities increased by 33.7% and 25.9%, respectively, compared with those of free THI. Furthermore, the results showed that the transport efficiency of THI@BSA·NPs was highest at a concentration of 50 μg/mL, and the THI@BSA·NPs content in the trunk, from to lower to higher layers, was 8.8, 8.2, 7.6, and 5.8 μg/g. At the same time, THI@BSA·NPs also exhibited high transport efficiency in dead trees. Conclusion The transport efficiency and toxicity of the active ingredients are the key factors for the control of TBPs. This work provided idea for the application of biological delivery system encapsulated hydrophobic pesticides. The novel self-assembled THI@BSA·NPs have promising potential for sustainable control of TBPs.


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