Dynamic mechanical measurement of the viscoelasticity of single adherent cells

2016 ◽  
Vol 108 (9) ◽  
pp. 093701 ◽  
Author(s):  
Elise A. Corbin ◽  
Olaoluwa O. Adeniba ◽  
Randy H. Ewoldt ◽  
Rashid Bashir
Keyword(s):  
Adherent Cells ◽  
Mechanical Measurement ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Measurement

scholarly journals Computer aided system for dynamic mechanical measurement.

1988 ◽  
Vol 35 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Kunio KUROKOUCHI ◽  
Junichi SUGIYAMA ◽  
Hisaya HORIUCHI
Keyword(s):  
Mechanical Measurement ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Measurement ◽  
Computer Aided

Dynamic mechanical measurement of ??n

1960 ◽  
Vol 44 (143) ◽  
pp. 149-154 ◽  
Author(s):  
W. P. Cox ◽  
R. A. Isaksen ◽  
E. H. Merz
Keyword(s):  
Mechanical Measurement ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Measurement

On-Chip Dynamic Mechanical Measurement

2019 ◽  
Author(s):  
Atsushi Kirimoto ◽  
Hiroaki Ito ◽  
Mitsuhiro Horade ◽  
Toshio Takayama ◽  
Misato Chimura ◽  
...  
Keyword(s):  
Mechanical Measurement ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Measurement ◽  
On Chip

scholarly journals A computational model of the response of adherent cells to stretch and changes in substrate stiffness

2014 ◽  
Vol 116 (7) ◽  
pp. 825-834 ◽  
Author(s):  
Harikrishnan Parameswaran ◽  
Kenneth R. Lutchen ◽  
Béla Suki
Keyword(s):  
Molecular Motors ◽  
Cellular Response ◽  
Mechanical Stretch ◽  
The Body ◽  
Cyclic Stretch ◽  
Substrate Stiffness ◽  
Adherent Cells ◽  
Actin Network ◽  
Mechanical Environment ◽  
Dynamic Mechanical

Cells in the body exist in a dynamic mechanical environment where they are subject to mechanical stretch as well as changes in composition and stiffness of the underlying extracellular matrix (ECM). However, the underlying mechanisms by which cells sense and adapt to their dynamic mechanical environment, in particular to stretch, are not well understood. In this study, we hypothesized that emergent phenomena at the level of the actin network arising from active structural rearrangements driven by nonmuscle myosin II molecular motors play a major role in the cellular response to both stretch and changes in ECM stiffness. To test this hypothesis, we introduce a simple network model of actin-myosin interactions that links active self-organization of the actin network to the stiffness of the network and the traction forces generated by the network. We demonstrate that such a network replicates not only the effect of changes in substrate stiffness on cellular traction and stiffness and the dependence of rate of force development by a cell on the stiffness of its substrate, but also explains the physical response of adherent cells to transient and cyclic stretch. Our results provide strong indication that network phenomena governed by the active reorganization of the actin-myosin structure plays an important role in cellular mechanosensing and response to both changes in ECM stiffness and externally applied mechanical stretch.

Correlation Between Thermal Diffusivity and Dynamic Mechanical Properties of Soft Magnetic Particle Filled Thermoplastic Composites

2011 ◽  
Author(s):  
S. Kirchberg ◽  
M. Anhalt ◽  
B. Weidenfeller
Keyword(s):  
Thermal Diffusivity ◽  
Storage Modulus ◽  
Filler Particle ◽  
Free Path Length ◽  
Dynamic Mechanical Properties ◽  
Thermoplastic Composites ◽  
Flash Method ◽  
Dynamic Mechanical ◽  
Dynamic Mechanical Measurement ◽  
Filler Fraction

Measurements of thermal diffusivity α by laser flash method (LFA) and storage modulus E′ by dynamic mechanical analysis (DMA) have been performed on polypropylene-iron silicon (PP/FeSi6.8) composites with filler particle content from 10 vol.-% to 60 vol.-% at temperatures from 300 K to 415 K and 200 K to 425 K, respectively. The thermal diffusivity induces a decline with increased temperature for all examined materials. The drop in thermal diffusivity versus temperature corresponds with the filler fraction. Observing the behavior of the storage modulus of FeSi6.8 filled PP one can show that the larger the filler fraction of particles in the polymer the stronger is the decrease of storage modulus versus temperature. The inflection point of the dynamic mechanical measurement curve at about 360 K corresponds very well with starting increase of thermal conductivity. The achieved results can be explained by the relation of the velocity v of phonons and its free path length l to the thermal diffusivity α according to α = (1/3)vl (Einstein approximation). Moreover, v is correlated to the bulk modulus K (and specific density ρ) via v ≈ (K/ρ)0.5.

Application of Liquefied Wood to a New Resin System—Synthesis and Properties of Liquefied Wood/Epoxy Resins

Holzforschung ◽  
2000 ◽  
Vol 54 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Masahiko Kobayashi ◽  
Kyoko Tukamoto ◽  
Bunichiro Tomita
Keyword(s):  
Glass Transition ◽  
Epoxy Resins ◽  
Polymer Networks ◽  
Resin System ◽  
Optimum Conditions ◽  
Mechanical Measurement ◽  
Liquefied Wood ◽  
Dynamic Mechanical Measurement ◽  
Film Forming ◽  
Wood Content

Summary A new resin system was developed by reacting liquefied wood with various epoxy compounds. Examination of the optimum conditions for the synthesis of the resins, and the behavior of liquefied wood in the cured resins were undertaken. Generally, the compatibility and film forming performance of liquefied wood/epoxy resins became better as the wood content increased. The presence of only one broad dispersion peak, due to the glass transition, was observed during dynamic mechanical measurement of resin films suggesting that the different components of the resin were compatible. The glass transition temperature of the cured resins could be modified by changing the kinds of epoxy compounds and the wood contents of liquefied wood. The presence of rubber elasticity at higher temperatures suggests that the resin systems consisted of cross-linking polymer networks.

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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