scholarly journals Characterizing nanoscale scanning probes using electron microscopy: A novel fixture and a practical guide

2016 ◽  
Vol 87 (1) ◽  
pp. 013703 ◽  
Author(s):  
Tevis D. B. Jacobs ◽  
Graham E. Wabiszewski ◽  
Alexander J. Goodman ◽  
Robert W. Carpick
Keyword(s):  
Electron Microscopy ◽  
Scanning Probes ◽  
Practical Guide

scholarly journals From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

10.3791/51673 ◽  
2014 ◽  
Author(s):  
Wen-Ting Tsai ◽  
Ahmed Hassan ◽  
Purbasha Sarkar ◽  
Joaquin Correa ◽  
Zoltan Metlagel ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Practical Guide ◽  
3D Data

A Practical Guide to Bright/Dark Field Scanning Transmission Electron Microscopy

2012 ◽  
Vol 18 (S2) ◽  
pp. 1964-1965
Author(s):  
P.J. Phillips ◽  
M. De Graef ◽  
M.J. Mills
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Dark Field ◽  
Scanning Transmission Electron ◽  
Practical Guide ◽  
Transmission Electron ◽  
Scanning Transmission

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

scholarly journals Molecular dynamics flexible fitting: A practical guide to combine cryo-electron microscopy and X-ray crystallography

Methods ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 174-180 ◽  
Author(s):  
Leonardo G. Trabuco ◽  
Elizabeth Villa ◽  
Eduard Schreiner ◽  
Christopher B. Harrison ◽  
Klaus Schulten
Keyword(s):  
Electron Microscopy ◽  
Molecular Dynamics ◽  
X Ray ◽  
X Ray Crystallography ◽  
Practical Guide ◽  
Cryo Electron Microscopy

A practical guide to transmission electron microscopy of aerogels

2004 ◽  
Vol 350 ◽  
pp. 277-284 ◽  
Author(s):  
Rhonda M. Stroud ◽  
Jeffrey W. Long ◽  
Jeremy J. Pietron ◽  
Debra R. Rolison
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Practical Guide ◽  
Transmission Electron

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

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