A prospective study on evaluation of pathogenesis, biofilm formation, antibiotic susceptibility of microbial community in urinary catheter

2015 ◽  
Author(s):  
Khansa Mohammed Younis ◽  
Gires Usup ◽  
Asmat Ahmad
Keyword(s):  
Microbial Community ◽  
Prospective Study ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Urinary Catheter ◽  
A Prospective Study

scholarly journals Spectrum of bacteria associated with diabetic foot ulcer and biofilm formation: A prospective study

2015 ◽  
pp. 280-285 ◽  
Author(s):  
Asima Banu ◽  
Mir Mohammad Noorul Hassan ◽  
Janani Rajkumar ◽  
Sathyabheemarao Srinivasa
Keyword(s):  
Prospective Study ◽  
Diabetic Foot ◽  
Biofilm Formation ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
A Prospective Study

scholarly journals BIOFILM FORMATION AND ANTIBIOTIC SUSCEPTIBILITY OF STROKE PATIENT’S URINARY CATHETER ISOLATES AT BETHESDA HOSPITAL, YOGYAKARTA

2019 ◽  
Vol 4 (2) ◽  
pp. 58
Author(s):  
Christofer Sathya Wijaya Budi Sarwono
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Biofilm Formation ◽  
Urinary Catheter ◽  
Cross Sectional Study ◽  
Stroke Patients ◽  
Plate Method ◽  
Cross Sectional ◽  
E Coli

Background: Antibiotic resistance currently challenges infectious disease management, specifically in combating biofilm formation. Biofilm might be developed on urinary catheter, in which stroke patients with urinary tract problem have higher risk of catheter associated urinary tract infection and problems related to antibiotic resistance. Aim: This study describes the microbe types from catheter isolates, measuring the antibiotic susceptibility and biofilm formation, especially in stroke patients at Bethesda Hospital, Yogyakarta. Method: This is a cross-sectional study, describing findings of specimens from stroke patients at Bethesda Hospital, since December 2018 to January 2019. The isolates were identified with standard method, the antibiotic susceptibility were tested with antibiotic disc on MHA medium, and the biofilm formation were assessed using tissue culture plate method. All procedure were done in Microbiology Laboratorium, FK UKDW. Result: Thirty three microbes were isolated from 30 specimens of urinary catheter (63,3% male, 36,3% female). There is 76% isolates incapable of developing biofilm, while 15% is moderate biofilm producer and 9% is strong biofilm producer. The susceptibility test showed 100% resistancy of ampicillin-sulbactam, tetracyclin, ketoconazole, itraconazole, and terbinafine. Around 50% of E. coli and 57% of K. pneumonias isolates is MDR, and 75% of A. baumanii is XDR. Conclusion: Isolated microbes from urinary catheter of stroke patients at Bethesda Hospital is dominated by E. coli, K. pneumoniae, and A. baumanii. Some of the microbes could form strong biofilm, and some of the antibiotics could not be used in the future due to their 100% resistancy on all specimens.

The Impact of a Cation Channel Blocker (Furosemide) on Pseudomonas aeruginosa PA01 Biofilm Architecture

2007 ◽  
Vol 137 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Jenny L. Cross ◽  
Hassan H. Ramadan ◽  
John G. Thomas
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prospective Study ◽  
Study Design ◽  
Biofilm Formation ◽  
Channel Blocker ◽  
Minimal Effect ◽  
Biofilm Architecture ◽  
Ph Dependent ◽  
A Prospective Study ◽  
The Impact

OBJECTIVE: To determine if furosemide could have a role in inhibition of biofilm formation. STUDY DESIGN: A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls. METHODS: Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2-fold dilutions of furosemide and controls over a 48-hour period at pH concentrations of 7.0, 8.0, and 9.0. RESULTS: Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10 mg/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to <30% at concentrations of 0.6 to 0.16 mg/mL at pH 8.0 and <10% at concentrations of 1.25 to 0.3 mg/mL at pH 9.0. CONCLUSION: Furosemide was found to be nonantiplanktonic, but does appear to destabilize preformed PA01 biofilms at pH-dependent concentrations.

Anxiety, depression and stressful life events among medical students: a prospective study in Antalya, Turkey

2001 ◽  
Vol 35 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Mehmet Aktekin ◽  
Taha Karaman ◽  
Yesim Yigiter Senol ◽  
Sukru Erdem ◽  
Hakan Erengin ◽  
...  
Keyword(s):  
Medical Students ◽  
Prospective Study ◽  
Life Events ◽  
Stressful Life Events ◽  
Stressful Life ◽  
A Prospective Study ◽  
Anxiety Depression
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